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mmu_circIno80d_1801
Basic information of circular RNA
PanCircBase ID:
mmu_circIno80d_1801
mm39_Chr. Position ID:
chr1|63097123|63107452|-
Gene name:
Ino80d
Transcript:
ENSMUST00000133236.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
9,8,7,6,5
Exon/intron offsets:
0,3075,4071,7739,10219
Genomic length:
10329
Exon/intron sizes in nt:
1,18,11,58,21,81,34,110
Flanking exon/introns:
chr1:63020539-63097123|chr1:63107452-63113480
Splice length:
1801
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CCTTCGCCAGACCAAGTTGAAGGAGGTGGAGCCAGCAGCATGTAGTGGAACAGTGAAGGGCGAACAGTGCACAAAGCAGGCCCTTCCCTTCACCAGACACTGTTTCCAGCACATCCTCCTGAACCGCTCTCAGCAGCTCTTCTCCAGCTGCACTGCCAAGTTTGCAGACGGACAGCAGTGCTCTGTGCCAGTGTTTGACATTACACACCAGACGCCCCTGTGTGAAGAACATGCCAAAAAGATGGATAATTTCCTGAGAGGAGATAACTCCCGTAAAGTTCAGCACCAGCAACAGAGGAAACCTAGGAAAAAAACGAAGCCCCCTGCACTTACCAAAAAACACAAGAAGAAGAGACGGCGTGGACCTCGTCGGCCCCAGAAGCCCATCCCCCCTGCAGTGCCCCAAGGGAACCTCAGCATGCCCACCAGCGTCTCACTGCCAGTGGAGGCCTCACAGATGCGAAGCCCATCCACGCCCGAGCTGAGTGCTGATGAGTTGCCGGATGACATTGCCAACGAGATCACCGACATCCCGCACGACCTGGAACTGAACCAGGAGGACTTTGCTGATGTCCTGCCTCGGCTGCCGGATGATCTACAAGACTTTGATTTTTTTGAAGGAAAGAACGGAGACCTCCTCCCGACCACAGAAGAAGCCGAAGAGCTCGAGCGGGCCTTGCAAGCTGTAACTTCTCTCGAGTGCCTGAGTACAATTGGGGTACTTTCCCAGTCAGACGGTGTGCCCGTTCAGGGGTTGTCAGATAGAGGAATGGGGGTGTTCTCCACGGGTACTGATGCTTCGGGAATCCAGTCCTTGAGTCGAGAAGTAAACACGGACCTAGGGGAGCTACTGAATGGACGTATAGTGCACGATAGCTTTTCTAGTCTGGAGCTGGACGAGAACCTACTCCATTCTGCTCCTCTGTCCAGCCCACCCACAGCCCTGGCAGGGCAGATCCAGGGGCAGTTCTCTGCCCCAGCCAGCGCTGGCCTTACTTCTGCCACTCTGCTCAGCCAGAGTGCGCTTGGGGAGAGAGCCTTCCCAGGACAGTTTCATGGCCTTCATGATGGCAGCCATGCCTCCCAGAGGCCACATCCTGCCCAGCTGCTGAGCAAGGCAGACGACCTTATCACCTCACGACAGCAATACAGCAGTGATCATTCACACTCCTCACCCCATGGAAGCCATTATGATAGCGAGCACGTGCCGTCGCCCTACAGTGACCATATCACCTCTCCCCACACATCTTACTCTGGTGATAATATGGCAGCTACCTTTTCGGCAGAGATGCCCATCATGGCGCAGCACTTGCTCCCCACACAGCTCGAGGTGCCACTTGGAGGAGTGGTCAACCCCAGAACTCACTGGGGGAATCTCCCCGTCAACCTTGGCGACCCCTCTGCATTTAGCAACCTCCTTGGCGCGGACGGACACCTTCTTTCCACTTCCCTCTCCACGCCACCCACCACGTCCAACTCAGAGACCACACAGCCTGCCTTCGCCACCGTTACCCCAAGCAGTTCCAGTGTGCTTCCGGGGTTACCCCAGACCAGCTTCAGTGGCATGGGGCCTTCTGCTGAACTAATGGCCTCCACCTCTCCGAAGCAGCAACTCCCGCAGTTCAGTGCAGCCTTCGGCCACCAGCTGAGTTCTCACAGTGGCATTCCTAAGGACCTGCAGCCCAGCCACAGCTCCATAGCCCCTCCTACAGGGTTCACTGCAACAGGTGCCACGGCTACAAGTACCAACAATGCATCGCCCCCCTTCCCCAGCCCTAACTGAGCGCGTCTGTACTGGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTACCAACAATGCATCGCCC
59.9
GTGTCTGGTGAAGGGAAGGG
59.96
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCACGGCTACAAGTACCAA
60.32
GTGTCTGGTGAAGGGAAGGG
59.96
172
PCR template sequence:
CCCTCCTACAGGGTTCACTGCAACAGGTGCCACGGCTACAAGTACCAACAATGCATCGCCCCCCTTCCCCAGCCCTAACTGAGCGCGTCTGTACTGGCAGCCTTCGCCAGACCAAGTTGAAGGAGGTGGAGCCAGCAGCATGTAGTGGAACAGTGAAGGGCGAACAGTGCACAAAGCAGGCCCTTCCCTTCACCAGACAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
24
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(2), mmu-miR-34a-5p(2), mmu-miR-141-3p(1), mmu-miR-122-5p(1), mmu-miR-146a-5p(1), mmu-miR-33-5p(1), mmu-miR-148a-3p(1), mmu-miR-196a-5p(1), mmu-miR-709(1), mmu-miR-138-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-149-5p(1), mmu-miR-434-5p(1), mmu-miR-433-3p(1), mmu-miR-182-5p(1), mmu-miR-34b-5p(1), mmu-miR-680(1), mmu-miR-377-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-3470b(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGTACTGGCAGCCTTCGCCAGACC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINO80D_1801_ORF_1
1.012
0.265079105
0.999980904
1833
ORF sequence:
ATGCATCGCCCCCCTTCCCCAGCCCTAACTGAGCGCGTCTGTACTGGCAGCCTTCGCCAGACCAAGTTGAAGGAGGTGGAGCCAGCAGCATGTAGTGGAACAGTGAAGGGCGAACAGTGCACAAAGCAGGCCCTTCCCTTCACCAGACACTGTTTCCAGCACATCCTCCTGAACCGCTCTCAGCAGCTCTTCTCCAGCTGCACTGCCAAGTTTGCAGACGGACAGCAGTGCTCTGTGCCAGTGTTTGACATTACACACCAGACGCCCCTGTGTGAAGAACATGCCAAAAAGATGGATAATTTCCTGAGAGGAGATAACTCCCGTAAAGTTCAGCACCAGCAACAGAGGAAACCTAGGAAAAAAACGAAGCCCCCTGCACTTACCAAAAAACACAAGAAGAAGAGACGGCGTGGACCTCGTCGGCCCCAGAAGCCCATCCCCCCTGCAGTGCCCCAAGGGAACCTCAGCATGCCCACCAGCGTCTCACTGCCAGTGGAGGCCTCACAGATGCGAAGCCCATCCACGCCCGAGCTGAGTGCTGATGAGTTGCCGGATGACATTGCCAACGAGATCACCGACATCCCGCACGACCTGGAACTGAACCAGGAGGACTTTGCTGATGTCCTGCCTCGGCTGCCGGATGATCTACAAGACTTTGATTTTTTTGAAGGAAAGAACGGAGACCTCCTCCCGACCACAGAAGAAGCCGAAGAGCTCGAGCGGGCCTTGCAAGCTGTAACTTCTCTCGAGTGCCTGAGTACAATTGGGGTACTTTCCCAGTCAGACGGTGTGCCCGTTCAGGGGTTGTCAGATAGAGGAATGGGGGTGTTCTCCACGGGTACTGATGCTTCGGGAATCCAGTCCTTGAGTCGAGAAGTAAACACGGACCTAGGGGAGCTACTGAATGGACGTATAGTGCACGATAGCTTTTCTAGTCTGGAGCTGGACGAGAACCTACTCCATTCTGCTCCTCTGTCCAGCCCACCCACAGCCCTGGCAGGGCAGATCCAGGGGCAGTTCTCTGCCCCAGCCAGCGCTGGCCTTACTTCTGCCACTCTGCTCAGCCAGAGTGCGCTTGGGGAGAGAGCCTTCCCAGGACAGTTTCATGGCCTTCATGATGGCAGCCATGCCTCCCAGAGGCCACATCCTGCCCAGCTGCTGAGCAAGGCAGACGACCTTATCACCTCACGACAGCAATACAGCAGTGATCATTCACACTCCTCACCCCATGGAAGCCATTATGATAGCGAGCACGTGCCGTCGCCCTACAGTGACCATATCACCTCTCCCCACACATCTTACTCTGGTGATAATATGGCAGCTACCTTTTCGGCAGAGATGCCCATCATGGCGCAGCACTTGCTCCCCACACAGCTCGAGGTGCCACTTGGAGGAGTGGTCAACCCCAGAACTCACTGGGGGAATCTCCCCGTCAACCTTGGCGACCCCTCTGCATTTAGCAACCTCCTTGGCGCGGACGGACACCTTCTTTCCACTTCCCTCTCCACGCCACCCACCACGTCCAACTCAGAGACCACACAGCCTGCCTTCGCCACCGTTACCCCAAGCAGTTCCAGTGTGCTTCCGGGGTTACCCCAGACCAGCTTCAGTGGCATGGGGCCTTCTGCTGAACTAATGGCCTCCACCTCTCCGAAGCAGCAACTCCCGCAGTTCAGTGCAGCCTTCGGCCACCAGCTGAGTTCTCACAGTGGCATTCCTAAGGACCTGCAGCCCAGCCACAGCTCCATAGCCCCTCCTACAGGGTTCACTGCAACAGGTGCCACGGCTACAAGTACCAACAATGCATCGCCCCCCTTCCCCAGCCCTAACTGA
Peptide sequence:
MHRPPSPALTERVCTGSLRQTKLKEVEPAACSGTVKGEQCTKQALPFTRHCFQHILLNRSQQLFSSCTAKFADGQQCSVPVFDITHQTPLCEEHAKKMDNFLRGDNSRKVQHQQQRKPRKKTKPPALTKKHKKKRRRGPRRPQKPIPPAVPQGNLSMPTSVSLPVEASQMRSPSTPELSADELPDDIANEITDIPHDLELNQEDFADVLPRLPDDLQDFDFFEGKNGDLLPTTEEAEELERALQAVTSLECLSTIGVLSQSDGVPVQGLSDRGMGVFSTGTDASGIQSLSREVNTDLGELLNGRIVHDSFSSLELDENLLHSAPLSSPPTALAGQIQGQFSAPASAGLTSATLLSQSALGERAFPGQFHGLHDGSHASQRPHPAQLLSKADDLITSRQQYSSDHSHSSPHGSHYDSEHVPSPYSDHITSPHTSYSGDNMAATFSAEMPIMAQHLLPTQLEVPLGGVVNPRTHWGNLPVNLGDPSAFSNLLGADGHLLSTSLSTPPTTSNSETTQPAFATVTPSSSSVLPGLPQTSFSGMGPSAELMASTSPKQQLPQFSAAFGHQLSSHSGIPKDLQPSHSSIAPPTGFTATGATATSTNNASPPFPSPN
IRESfinder result
IRESfinder score
0.455236093
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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