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mmu_circIkbke_687
Basic information of circular RNA
PanCircBase ID:
mmu_circIkbke_687
mm39_Chr. Position ID:
chr1|131184623|131193687|-
Gene name:
Ikbke
Transcript:
ENSMUST00000062108.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
21,20,19,18,17,16,15,14
Exon/intron offsets:
0,230,1014,2295,3119,6386,8752,8988
Genomic length:
9064
Exon/intron sizes in nt:
72,107,97,102,40,77,116,76
Flanking exon/introns:
chr1:131183075-131184623|chr1:131193687-131197537
Splice length:
687
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCAGCAGTGGAGCGGGGATGCCTGACGTCCAGGAACGAAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAATGTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATGAGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAAGAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTCAGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGGTCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGTGGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTGGACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGAAGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAACAACCGACTCATCGAACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTTCCACATGCAGGAGCT
60.04
CTGCAGCCTGGTTCTTAGCT
60.04
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACCTGGTCTTCCACATGCA
59.96
TCTGTGGCCTCCTTTCGTTC
59.97
140
PCR template sequence:
GCCCTGATCCGAAGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAACAACCGACTCATCGAACGGTTCAGCAGTGGAGCGGGGATGCCTGACGTCCAGGAACGAAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GACTCATCGAACGGTTCAGCAGTGGA
siRNA sets
CTGAACCGTTCGATGAGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCIKBKE_687_ORF_1
1.3114
0.363339377
0.999778032
1353
ORF sequence:
ATGCCTGACGTCCAGGAACGAAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAATGTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATGAGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAAGAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTCAGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGGTCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGTGGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTGGACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGAAGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAACAACCGACTCATCGAACGGTTCAGCAGTGGAGCGGGGATGCCTGACGTCCAGGAACGAAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAATGTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATGAGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAAGAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTCAGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGGTCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGTGGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTGGACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGAAGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAACAACCGACTCATCGAA
Peptide sequence:
MPDVQERKEATELRTRLQTLSEILSKCSHNVTETQRSLSCLGEELLKNRDQIHEDNKSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQTYQVSLVTHGKRMRQVQRAQNHLHLIGHSVATCNSEARGAQESLNKIFDQLLLDRASEQGAEVSPQPMAPHPGPDPKDLVFHMQELCNDMKLLAFDLQDNNRLIERFSSGAGMPDVQERKEATELRTRLQTLSEILSKCSHNVTETQRSLSCLGEELLKNRDQIHEDNKSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQTYQVSLVTHGKRMRQVQRAQNHLHLIGHSVATCNSEARGAQESLNKIFDQLLLDRASEQGAEVSPQPMAPHPGPDPKDLVFHMQELCNDMKLLAFDLQDNNRLIE
IRESfinder result
IRESfinder score
0.436278401
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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