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mmu_circIft57_69

Basic information of circular RNA
PanCircBase ID:

mmu_circIft57_69

mm39_Chr. Position ID:

chr16|49532201|49532270|+

Gene name:

Ift57

Transcript:

ENSMUST00000046777.11

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

1

Exon/intron indexes:

5

Exon/intron offsets:

0

Genomic length:

69

Exon/intron sizes in nt:

69

Flanking exon/introns:

chr16:49526544-49532201|chr16:49532270-49557034

Splice length:

69

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GAAGAGGAGACAGATAATGAAGAAAACTTTATTGATCTCAACGTTTTAAAGGCCCAGACCTATCGCTTG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TCAACGTTTTAAAGGCCCAGAC

59.38

AGTTTTCTTCATTATCTGTCTCCTCT

57.86

60

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CAACGTTTTAAAGGCCCAGACC

60.29

AAGTTTTCTTCATTATCTGTCTCCTCT

58.4

60

PCR template sequence:

TCTCAACGTTTTAAAGGCCCAGACCTATCGCTTGGAAGAGGAGACAGATAATGAAGAAAACTTTATTG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

1

Interacting miRNAs (#binding sites)

mmu-miR-335-3p(1)

siRNAs for circular RNA silencing
Junction sequence

GACCTATCGCTTGGAAGAGGAGACAG

siRNA sets

CTGTCTCCTCTTCCAAGCG, TGTCTCCTCTTCCAAGCGA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.721217119

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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Institute of Life sciences, Bhubaneswar, India

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