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mmu_circHuwe1_3547
Basic information of circular RNA
PanCircBase ID:
mmu_circHuwe1_3547
mm39_Chr. Position ID:
chrX|150663495|150684677|+
Gene name:
Huwe1
Transcript:
ENSMUST00000026292.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
20
Exon/intron indexes:
29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48
Exon/intron offsets:
0,578,1992,2579,5082,5583,6244,7884,9169,9490,10230,10517,11240,13323,14421,16063,16687,17944,20401,20999
Genomic length:
21182
Exon/intron sizes in nt:
21,71,23,23,82,31,12,30,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chrX:150661905-150663495|chrX:150684677-150686824
Splice length:
3547
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTTCCAGATTAGGCCGAGCACTTGCTGAGCTCTTTGGACTCCTTGTTAAGCTTTGTGTGGGTTCTCCTGTCCGCCAAAGGAGGAGCCATCATGCTGCCAGTACCACCACTGCACCAACACCTGCTGCTAGATCAACAGCTTCAGCCCTCACTAAGCTACTCACAAAAGGCTTATCATGGCAGCCCCCACCATATACACCCACTCCCCGCTTCAGGCTGACGTTCTTCATCTGTTCAGTTGGATTCACCTCTCCAATGCTCTTTGATGAGAGAAAGTATCCTTACCACCTTATGCTGCAGAAATTTCTCTGCTCTGGTGGTCACAATGCTCTTTTTGAAACTTTCAACTGGGCTCTATCCATGGGAGGAAAAGTTCCTGTTTCTGAGGGATTGGAACATTCAGATCTACCTGATGGTACAGGAGAATTCCTAGATGCCTGGCTTATGCTTGTAGAAAAGATGGTAAATCCCACCACGGTGCTTGAATCTCCACATTCGTTGCCTGCTAAATTGCCTGGAGGAGTCCAGAGCTTTCCACAATTTAGTGCCCTTCGCTTCCTTGTAGTAACTCAAAAAGCAGCCTTTACTTGTATCAAGAACCTGTGGAACCGGAAACCCCTTAAAGTATATGGGGGAAGAATGGCTGAGTCTATGCTGGCCATTTTGTGCCACATTCTCCGAGGGGAACCTGTGATTCGAGAAAGACTGAGCAAAGAGAAGGAAGGATCTCGAGGAGAAGAAGAAGCAGGCCAGGAAGAAGGTGGCTCACGCAGAGAACCTCAAGTCAACCAGCAACAGCTACAACAGCTCATGGACATGGGTTTCACAAGAGAACATGCCATGGAGGCACTGTTGAATACCAGTACGATGGAACAAGCAACAGAGTACCTTTTAACCCACCCTCCTCCAATCATAGGAGGAGTTGTTCGGGATCTTAGCATGTCAGAAGAGGACCAGATGATGAGAGCAATTGCTATGTCTCTGGGACAGGATATTCCAATGGATCAAAGGGCAGAGTCACCTGAGGAAGTTGCTTGCCGGAAGGAGGAAGAAGAGCGTAAAGCTCGAGAAAAACAAGAAGAGGAAGAAGCTAAATGTCTAGAGAAGTTTCAAGATGCTGACCCCTTGGAACAAGATGAGCTCCACACTTTCACAGATACAATGTTACCAGGCTGCTTCCATCTTCTTGATGAGTTGCCTGATACAGTGTACCGTGTTTGTGATCTGATCATGACAGCTATCAAACGTAATGGAGCTGACTATCGTGATATGATTCTAAAGCAAGTTGTCAACCAGGTGTGGGAAGCTGCTGATGTATTGATCAAAGCTGCTCTTCCTCTAACAACAAGTGACACAAAAACAGTTTCAGAGTGGATCAGTCAGATGGCTACCCTGCCACAGGCCTCCAATTTGGCTACTAGAATCTTGCTTTTAACACTACTTTTTGAGGAGTTGAAGTTACCTTGTGCCTGGGTGGTTGAATCAAGTGGCATTCTTAATGTCCTAATCAAACTCTTAGAAGTGGTTCAACCCTGCCTACAGGCTGCCAAAGAGCAAAAGGAAGTCCAGACCCCAAAGTGGATCACCCCAGTGTTGCTCCTTATTGATTTTTATGAAAAGACAGCCATCTCATCAAAGAGAAGAGCCCAAATGACTAAGTACCTACAGTCCAACAGCAACAACTGGCGCTGGTTTGATGACCGCTCTGGGCGTTGGTGTAGTTACAGTGCTAGCAACAACAGCACCATTGATTCTGCCTGGAAATCTGGAGAAACAAGTGTAAGATTCACTGCTGGCCGACGAAGATACACTGTCCAGTTCACTACAATGGTGCAGGTTAATGAAGAAACAGGAAACCGACGCCCTGTGATGCTGACTCTCCTCAGGGTGCCACGACTGAGTAAGAACTCAAAAAGCAGCAATGGACAGGAACTAGAGAAGACACTGGAAGAAAGCAAGGAGACCGATATCAAACATAAAGAAAATAAAGGCAATGATATCCCCTTAGCTCTAGAGAGCACAAACACTGAAAAAGAGGCAAGCCTGGATGAAACAAAAATTGGGGAGATCCTAATCCAGGGCCTGACAGAAGATATGGTGACTGTTCTAATCCGTGCCTGTGTGAGCATGCTGGGAGTCCCTGTGGACCCAGACACTTTGCATGCCACCCTTCGTCTCTGTTTAAGGCTCACCCGAGACCACAAATATGCCATGATGTTTGCAGAACTGAAGAGTACACGAATGATCTTGAATTTGACCCAGAGCTCAGGTTTCAATGGGTTTACCCCCTTAGTCACCCTTCTCTTAAGGCACATCATTGAGGATCCTTGTACTCTTCGTCATACTATGGAAAAGGTTGTTCGCTCGGCAGCCACAAGTGGAGCTGGTAGTACTACATCTGGTGTTGTATCTGGCAGCCTCGGTTCTCGGGAGATCAACTATATCCTTAGAGTCCTAGGACCAGCTGCCTGCCGCAATCCAGACATATTCACAGAAGTAGCCAATTGCTGTATCCGCATTGCCCTTCCAGCCCCTCGAGGCTCAGGAACTGCTTCAGATGATGAATTTGAGAATCTTAGAATTAAAGGTCCTAATGCTGTACAGCTGGTAAAGACAACACCTTTGAAGCCCTCATCTCTGCCTGTCATTCCTGATACTATTAAGGAAGTTATCTATGATATGCTGAATGCTTTGGCTGCATACCATGCTCCAGAGGAAGCAGACAAATCTGATCCTAAACCTGGGGGAACGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGACGATGTATACCAGCAGTACCGGTCACTTACTCGTCAGAGCAGTGACTTTGATACACAGTCAGGTTTTTCCTTAAATAGTCAGGTTTTTGCTGCAGATGGTGCCCCTGCTGAAACTTCCACAACTGGAACCTCCCAAGGAGAGGGAGCTTCAACTCCAGAGGAGACTCGAGAAGGGAAGAAAGATAAAGAAGGGGACCGTACATCTGAGGAAGGCAAGCAGAAAAGCAAGGGCAGCAAACCTTTAATGCCTACCTCTACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCAAACTACAGCTATACCGTGGGCCAGTCTGAGCTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTCCTGGACCACCTCCTCCCACACACCCAGAATGCAGAAGACAAGGATACACCTGCCCTGGCACGTCTATTCCTTGCAAGTCTGGCTGCTGCGGGGAGTGGCACAGATGCTCAAGTGGCCCTAGTGAATGAAGTGAAAGCAGCCCTGGGACGGGCGCTTGCTATGGCTGAGAGTACAGAGAAGCACGCCAGACTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAATCCTGCCCCTCCACCTCCAGCTTCTACAGTAGTGCTACAGCCAAGACTCAGCATAATGGCATGAACAACATCATTCGACTGTTCCTAAAGAAGGGACTAGTCAATGACCTGGCCAGAGTTCCTCACAGCCTAGACCTGTCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCAATGACCTGGCCAGAGT
59.67
CAGCATGATGGCTCCTCCTT
59.82
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AATGACCTGGCCAGAGTTCC
59.67
CAGCATGATGGCTCCTCCTT
59.82
140
PCR template sequence:
AGACTCAGCATAATGGCATGAACAACATCATTCGACTGTTCCTAAAGAAGGGACTAGTCAATGACCTGGCCAGAGTTCCTCACAGCCTAGACCTGTCCAGCTTCTTCCAGATTAGGCCGAGCACTTGCTGAGCTCTTTGGACTCCTTGTTAAGCTTTGTGTGGGTTCTCCTGTCCGCCAAAGGAGGAGCCATCATGCTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-433-3p(2), mmu-miR-15a-5p(1), mmu-miR-199a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-15b-5p(1), mmu-miR-381-3p(1), mmu-miR-495-3p(1), mmu-miR-214-3p(1), mmu-miR-136-5p(1), mmu-miR-434-3p(1), mmu-miR-431-5p(1), mmu-miR-351-5p(1), mmu-miR-382-3p(1), mmu-miR-212-3p(1), mmu-miR-382-5p(1), mmu-miR-541-5p(1), mmu-miR-324-3p(1), mmu-miR-383-5p(1), mmu-miR-705(1), mmu-miR-342-3p(1), mmu-miR-410-3p(1), mmu-miR-207(1), mmu-miR-539-5p(1), mmu-miR-350-3p(1), mmu-miR-1904(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGACCTGTCCAGCTTCTTCCAGATT
siRNA sets
AAGAAGCTGGACAGGTCTA,GAAGAAGCTGGACAGGTCT,TGGAAGAAGCTGGACAGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHUWE1_3547_ORF_1
0.9887
0.191520009
0.999999999
3321
ORF sequence:
ATGCTCTTTGATGAGAGAAAGTATCCTTACCACCTTATGCTGCAGAAATTTCTCTGCTCTGGTGGTCACAATGCTCTTTTTGAAACTTTCAACTGGGCTCTATCCATGGGAGGAAAAGTTCCTGTTTCTGAGGGATTGGAACATTCAGATCTACCTGATGGTACAGGAGAATTCCTAGATGCCTGGCTTATGCTTGTAGAAAAGATGGTAAATCCCACCACGGTGCTTGAATCTCCACATTCGTTGCCTGCTAAATTGCCTGGAGGAGTCCAGAGCTTTCCACAATTTAGTGCCCTTCGCTTCCTTGTAGTAACTCAAAAAGCAGCCTTTACTTGTATCAAGAACCTGTGGAACCGGAAACCCCTTAAAGTATATGGGGGAAGAATGGCTGAGTCTATGCTGGCCATTTTGTGCCACATTCTCCGAGGGGAACCTGTGATTCGAGAAAGACTGAGCAAAGAGAAGGAAGGATCTCGAGGAGAAGAAGAAGCAGGCCAGGAAGAAGGTGGCTCACGCAGAGAACCTCAAGTCAACCAGCAACAGCTACAACAGCTCATGGACATGGGTTTCACAAGAGAACATGCCATGGAGGCACTGTTGAATACCAGTACGATGGAACAAGCAACAGAGTACCTTTTAACCCACCCTCCTCCAATCATAGGAGGAGTTGTTCGGGATCTTAGCATGTCAGAAGAGGACCAGATGATGAGAGCAATTGCTATGTCTCTGGGACAGGATATTCCAATGGATCAAAGGGCAGAGTCACCTGAGGAAGTTGCTTGCCGGAAGGAGGAAGAAGAGCGTAAAGCTCGAGAAAAACAAGAAGAGGAAGAAGCTAAATGTCTAGAGAAGTTTCAAGATGCTGACCCCTTGGAACAAGATGAGCTCCACACTTTCACAGATACAATGTTACCAGGCTGCTTCCATCTTCTTGATGAGTTGCCTGATACAGTGTACCGTGTTTGTGATCTGATCATGACAGCTATCAAACGTAATGGAGCTGACTATCGTGATATGATTCTAAAGCAAGTTGTCAACCAGGTGTGGGAAGCTGCTGATGTATTGATCAAAGCTGCTCTTCCTCTAACAACAAGTGACACAAAAACAGTTTCAGAGTGGATCAGTCAGATGGCTACCCTGCCACAGGCCTCCAATTTGGCTACTAGAATCTTGCTTTTAACACTACTTTTTGAGGAGTTGAAGTTACCTTGTGCCTGGGTGGTTGAATCAAGTGGCATTCTTAATGTCCTAATCAAACTCTTAGAAGTGGTTCAACCCTGCCTACAGGCTGCCAAAGAGCAAAAGGAAGTCCAGACCCCAAAGTGGATCACCCCAGTGTTGCTCCTTATTGATTTTTATGAAAAGACAGCCATCTCATCAAAGAGAAGAGCCCAAATGACTAAGTACCTACAGTCCAACAGCAACAACTGGCGCTGGTTTGATGACCGCTCTGGGCGTTGGTGTAGTTACAGTGCTAGCAACAACAGCACCATTGATTCTGCCTGGAAATCTGGAGAAACAAGTGTAAGATTCACTGCTGGCCGACGAAGATACACTGTCCAGTTCACTACAATGGTGCAGGTTAATGAAGAAACAGGAAACCGACGCCCTGTGATGCTGACTCTCCTCAGGGTGCCACGACTGAGTAAGAACTCAAAAAGCAGCAATGGACAGGAACTAGAGAAGACACTGGAAGAAAGCAAGGAGACCGATATCAAACATAAAGAAAATAAAGGCAATGATATCCCCTTAGCTCTAGAGAGCACAAACACTGAAAAAGAGGCAAGCCTGGATGAAACAAAAATTGGGGAGATCCTAATCCAGGGCCTGACAGAAGATATGGTGACTGTTCTAATCCGTGCCTGTGTGAGCATGCTGGGAGTCCCTGTGGACCCAGACACTTTGCATGCCACCCTTCGTCTCTGTTTAAGGCTCACCCGAGACCACAAATATGCCATGATGTTTGCAGAACTGAAGAGTACACGAATGATCTTGAATTTGACCCAGAGCTCAGGTTTCAATGGGTTTACCCCCTTAGTCACCCTTCTCTTAAGGCACATCATTGAGGATCCTTGTACTCTTCGTCATACTATGGAAAAGGTTGTTCGCTCGGCAGCCACAAGTGGAGCTGGTAGTACTACATCTGGTGTTGTATCTGGCAGCCTCGGTTCTCGGGAGATCAACTATATCCTTAGAGTCCTAGGACCAGCTGCCTGCCGCAATCCAGACATATTCACAGAAGTAGCCAATTGCTGTATCCGCATTGCCCTTCCAGCCCCTCGAGGCTCAGGAACTGCTTCAGATGATGAATTTGAGAATCTTAGAATTAAAGGTCCTAATGCTGTACAGCTGGTAAAGACAACACCTTTGAAGCCCTCATCTCTGCCTGTCATTCCTGATACTATTAAGGAAGTTATCTATGATATGCTGAATGCTTTGGCTGCATACCATGCTCCAGAGGAAGCAGACAAATCTGATCCTAAACCTGGGGGAACGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGACGATGTATACCAGCAGTACCGGTCACTTACTCGTCAGAGCAGTGACTTTGATACACAGTCAGGTTTTTCCTTAAATAGTCAGGTTTTTGCTGCAGATGGTGCCCCTGCTGAAACTTCCACAACTGGAACCTCCCAAGGAGAGGGAGCTTCAACTCCAGAGGAGACTCGAGAAGGGAAGAAAGATAAAGAAGGGGACCGTACATCTGAGGAAGGCAAGCAGAAAAGCAAGGGCAGCAAACCTTTAATGCCTACCTCTACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCAAACTACAGCTATACCGTGGGCCAGTCTGAGCTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTCCTGGACCACCTCCTCCCACACACCCAGAATGCAGAAGACAAGGATACACCTGCCCTGGCACGTCTATTCCTTGCAAGTCTGGCTGCTGCGGGGAGTGGCACAGATGCTCAAGTGGCCCTAGTGAATGAAGTGAAAGCAGCCCTGGGACGGGCGCTTGCTATGGCTGAGAGTACAGAGAAGCACGCCAGACTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAATCCTGCCCCTCCACCTCCAGCTTCTACAGTAGTGCTACAGCCAAGACTCAGCATAATGGCATGAACAACATCATTCGACTGTTCCTAAAGAAGGGACTAGTCAATGACCTGGCCAGAGTTCCTCACAGCCTAGACCTGTCCAGCTTCTTCCAGATTAGGCCGAGCACTTGCTGA
Peptide sequence:
MLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSFFQIRPSTC
IRESfinder result
IRESfinder score
0.561246611
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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