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mmu_circHectd4_3557
Basic information of circular RNA
PanCircBase ID:
mmu_circHectd4_3557
mm39_Chr. Position ID:
chr5|121455264|121482925|+
Gene name:
Hectd4
Transcript:
ENSMUST00000042614.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
23
Exon/intron indexes:
38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60
Exon/intron offsets:
0,1361,2763,4196,4990,5434,6716,7989,10883,11161,11863,12256,14474,15720,16221,17135,19388,22223,24671,25448,25891,26277,27481
Genomic length:
27661
Exon/intron sizes in nt:
82,20,31,36,18,31,65,10,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr5:121453569-121455264|chr5:121482925-121486481
Splice length:
3557
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TAGCTCAGAAGGACGGCCCTTTCGACTCGGCACTGGTGCCAACATGGAAAAAGTTGTGAAGATGGATCGAGACATGACCAAGGGTGGCTGCTGTGAAGTGATCACAGAAGAGGCCTCAGCCGCCCTCAGGAAAGCAACCAAGTGGGCACAGTCGGGCCTCATTGTCAGTGTCGGACCACCAGTGGAATCCATCAACCCAGAGACTGTGAGTGGGCTGTCCACAGGTGACAAGAAGAAAACTGCCCAAACCTCCATCTGCCGAGAAAGAAACTCTGAACTGGCCAGAACTGACCCCGTGCGACCCTTCATCAGTGGACATGTGGCAAACAGCATGGCTGCAGAAGTGATCGCCTTGCTTCATAGCCTGCTAATGGCGCCAGAATCTAATGCTGCTCAAATATGGACCACAACGGCAGAAAAGGTTCTGTCTCGAGCATTGATGTACATTCCACAACTAGGGAAGTATGCAGAAAGCATCCTGGAGAATGGGAGCAGCAGTGGCAGGAAACTGGCCAAGCTTCAGAGGATTGCACGCCAGGCAGTTGCCGCCCTCTGTGCACTCGGAGGCTTTAAAGAGACAATCAAGATAGGCTCCGAGGTCCAGGTTTTAGGTAGAGGAATATCAGGAAGCATTGGAGTTGTGGCTTCTATCAATGAACAGGAAGGTATAGCTACAGTCCGGTTCCCTCCTGTGGACTGCCGAAGGACGTCACAAGCAGCAGACACGCTGACTATTCCATTGTCTAGACTTTGTGTTCCAAGGTCAGAGGCACTGCCTCTTCATAAGCTGTCCATTACTGAGAAGGTAGTCCAGGCAGTGCAGTCCATGCTGCTCCCTCAGGAAGGAAGCCTCTCTATCCACACCTCTCTTCCTGCAACAGGAGATGGCTCAGCACCTGTCATGGCAGTTGTCCGGCTTCTGGCTGAAATCAGAACCAGAGCATGCCTGGTCATGGCACAGCTTTTAGAAGACAGCCTGTTTTGTGAAGAGTTCATACAGCAGTGTCCAGCAGCTGTGGAAGTTCTGAACCTCGTAGCCCAGGAGTGCAGTGCGGGAGAGCGGCTGGCAGTTGTAGAGATGCAGTGTGAGAGGCTCAGGATGCTCTACCGGGACTGCGCTCGGCCCCCTCCACCTCCGCTGCAGGCTGACCGGAGACAGCCCAAAGAGATCACCTGGAGCCCTTCTCGAGTGTTCCCTCCAGTTCGTGCCTGCATGTTCTCATCTCACCTCACCTCTGTGACCTTCCTGGCTGACCCCAGCGCTGGAGGAGGTCTTCCCCGGGGCACATTCATCTATGCCACCTCGCCCTTGCCTGTTCAGGCCCCATCTTTCTACTGGGAAATTGAGATTGTTTCCTATGGAGACACTGATGATGACACTGGACCAATCGTCTCATTTGGCTTCGCTACAGAAGCTGAGAAGCGAGATGGGGCATGGACCAATCCAGTGGGCACTTGCCTCTTCCACAACAATGGCCGAGCTGTACACTACAATGGCTCCAGTCTACTGCAGTGGAAGAGCGTCCGCTTAGATGTCACACTCTCTCCTGGCGATGTGGCAGGAATCGGCTGGGAGAGGACCGAGGGGACTCCACCTCCTCCAGGGCAGCCAGCCAAAGGTCGGGTGTACTTCACATACTGCGGGCAGCGCCTCACACCATACCTAGAAGATGTCTCTGGTGGCATGTGGCCTGTGGTTCACATTCAGAAAAAGAACACAAAAACTCGAGCCAATTTTGGCTCCCGCCCATTTGCGTATGCTGAAGGACAGGCCCACCGAAATGCTGCCGACCTGTGCACTGACCTGGCAGAAGAGATCAGCGCTAACTTTGAGGCCTTGCCATTCGCCATGGCATCTGACAGTGACAACGATGCTGGCACCAGTATTGCATCAGACCCAGGCACTCATGGGCCGCCATGCCGGATTGCAGCAGTGGCCACAGCCCAGCAGCAATATGACAGTGACACCTCCTGCCACTATAAAGTAGAGCTCAGCTATGAGAATTTCATTACCTCAGGCCCAGACCCCCACCCACCTCCCATTGCGGATGATGAGAGTGATGATGACGACGATGATGACATCCCTCAGGAGGACCACTACGCCCTGCTGGTGAAAGCATGGGAGACCAAGGTTTTCCCTACCATCCGGAGACGCTTCCGCAATGAAGCCGAGCGAAAGTCGGGCCTAGACCAGATCAAGGGTGCTTTACAACTAGGCATGGTGGACATCGCCCGGCAGACAGTTGAGTTCCTCTATGAGGAGAATGGCGGCATCCCCAGAGACCTTTATCTTCCTACCATTGAGGACATTAAAGATGAAGCAAACAAGTTCACAATCGACAAAGTTCGAAAAGGTCTCACGGTTGTCACCCGCTCTCCAGACAGCAGCAATGTAGCTAGTAGTACCGTGGGAACTGCTCTGCCAAAATTTGCCATCCGAGGGATGCTAAAAACCTTCAGCCTTCATGGAGTCGTCCTAGATGTTGATTCAGTGAATGAATTGGTGCAGGTGGAGACATACCTGCGGAGTGAAGGGGTGTTGGTGCGGTACTGGTACCCCATTGATATGCTAGAAAGGCCCCCTGCAGGCTACCGGAGGACTGCCACCAATGGCCTGGTCACACTGGACAATACCAACCTTCAAATTCACAGAGAGCTGCTGCGATGTGAGGCTGCACTGGCCCGACTGTACTGTCGCATGGCCCTGCTTAACATCTTTGCCCCCAAGCTACCTCATCTATTCACTCGGCTCTTCCACATCCCTGCCATCCGAGACATCACGTTAGAGCACCTGCAGCTCCTGTCCAATCAGCTCCTTGCTCCTCCCCTCCCAGATGGCACCATCAGCTCCAGCTCCATCCTCCTGGCACAGTCTCTGCAGCACTGCATCCACTCCCAGAGCTGCTCTGCCACCGACCTCTTCTACCAGGGCAGCTCGCAGACACTGAGGGAGTGGCTCAGCGTGGCCATCACCCGCACCCTGCACCAAGGCGAGGACAGCCTCCTGGAGCTGACAAAGCAGATCTGCGCCTTCCTGCAGACAGCACCAGAGCAGTTCCCCTCCGAGGAATTCCCAGTGTCTGAGTCCAAAGTCAGTATGGATGTGAATTTCCCTGGAGCAGCTTTTGTTGTTGTGTCTTGCAAAGAAAGTCAGTCTGGATTCCGCAAAGACTCCTCTTTGTACAAGGCTCCGTGGGCACGCGTGCTCGTCTATGGGCTTGGCCACAAAGTGAAGCGTACCGGCCAGCTGAACCTCATTGAGGCTGCCTGCTACCCTCGAGACGCCTCCCCAGCCAACACTGGGCTCGCCCCTCCACCCACAGCTGACCAGTACCCTTCTGTGGTTCTTTCCACAGACAAAGTCCACATCAAACTAGGAGTGTCCCCACCACCTGGAGCAGTGCTGGTCTTGCATTCTCTGCCCCTGGAGTTCCCGCTGGCGATGGCCTTTGCCGAGCAGCTGCTGTCCTGGAAATCAGAGGACAGTGATGGGAAGTCAGAGGATGAGCCTGACAGCATCCCTGCCTCCGTCCTCCTGCACGTGGTGGAACTGCTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAAGTCAGAGGATGAGCC
59.53
CCACCCTTGGTCATGTCTCG
60.39
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GATGAGCCTGACAGCATCCC
60.53
CCACCCTTGGTCATGTCTCG
60.39
142
PCR template sequence:
CAGCTGCTGTCCTGGAAATCAGAGGACAGTGATGGGAAGTCAGAGGATGAGCCTGACAGCATCCCTGCCTCCGTCCTCCTGCACGTGGTGGAACTGCTGGTAGCTCAGAAGGACGGCCCTTTCGACTCGGCACTGGTGCCAACATGGAAAAAGTTGTGAAGATGGATCGAGACATGACCAAGGGTGGCTGCTGTGAAGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
33
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-miR-16-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-1a-3p(1), mmu-miR-199a-5p(1), mmu-miR-7b-5p(1), mmu-miR-206-3p(1), mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-miR-221-3p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-let-7c-5p(1), mmu-miR-322-5p(1), mmu-miR-217-5p(1), mmu-miR-222-3p(1), mmu-miR-320-3p(1), mmu-miR-143-3p(1), mmu-let-7d-5p(1), mmu-miR-503-5p(1), mmu-miR-382-3p(1), mmu-miR-22-3p(1), mmu-miR-96-5p(1), mmu-miR-324-3p(1), mmu-miR-107-3p(1), mmu-miR-705(1), mmu-miR-300-3p(1), mmu-miR-673-5p(1), mmu-miR-5101(1), mmu-miR-484(1), mmu-miR-574-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTGGAACTGCTGGTAGCTCAGAAGGA
siRNA sets
TCCTTCTGAGCTACCAGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHECTD4_3557_ORF_1
1.026
0.350397048
1
3573
ORF sequence:
ATGGAAAAAGTTGTGAAGATGGATCGAGACATGACCAAGGGTGGCTGCTGTGAAGTGATCACAGAAGAGGCCTCAGCCGCCCTCAGGAAAGCAACCAAGTGGGCACAGTCGGGCCTCATTGTCAGTGTCGGACCACCAGTGGAATCCATCAACCCAGAGACTGTGAGTGGGCTGTCCACAGGTGACAAGAAGAAAACTGCCCAAACCTCCATCTGCCGAGAAAGAAACTCTGAACTGGCCAGAACTGACCCCGTGCGACCCTTCATCAGTGGACATGTGGCAAACAGCATGGCTGCAGAAGTGATCGCCTTGCTTCATAGCCTGCTAATGGCGCCAGAATCTAATGCTGCTCAAATATGGACCACAACGGCAGAAAAGGTTCTGTCTCGAGCATTGATGTACATTCCACAACTAGGGAAGTATGCAGAAAGCATCCTGGAGAATGGGAGCAGCAGTGGCAGGAAACTGGCCAAGCTTCAGAGGATTGCACGCCAGGCAGTTGCCGCCCTCTGTGCACTCGGAGGCTTTAAAGAGACAATCAAGATAGGCTCCGAGGTCCAGGTTTTAGGTAGAGGAATATCAGGAAGCATTGGAGTTGTGGCTTCTATCAATGAACAGGAAGGTATAGCTACAGTCCGGTTCCCTCCTGTGGACTGCCGAAGGACGTCACAAGCAGCAGACACGCTGACTATTCCATTGTCTAGACTTTGTGTTCCAAGGTCAGAGGCACTGCCTCTTCATAAGCTGTCCATTACTGAGAAGGTAGTCCAGGCAGTGCAGTCCATGCTGCTCCCTCAGGAAGGAAGCCTCTCTATCCACACCTCTCTTCCTGCAACAGGAGATGGCTCAGCACCTGTCATGGCAGTTGTCCGGCTTCTGGCTGAAATCAGAACCAGAGCATGCCTGGTCATGGCACAGCTTTTAGAAGACAGCCTGTTTTGTGAAGAGTTCATACAGCAGTGTCCAGCAGCTGTGGAAGTTCTGAACCTCGTAGCCCAGGAGTGCAGTGCGGGAGAGCGGCTGGCAGTTGTAGAGATGCAGTGTGAGAGGCTCAGGATGCTCTACCGGGACTGCGCTCGGCCCCCTCCACCTCCGCTGCAGGCTGACCGGAGACAGCCCAAAGAGATCACCTGGAGCCCTTCTCGAGTGTTCCCTCCAGTTCGTGCCTGCATGTTCTCATCTCACCTCACCTCTGTGACCTTCCTGGCTGACCCCAGCGCTGGAGGAGGTCTTCCCCGGGGCACATTCATCTATGCCACCTCGCCCTTGCCTGTTCAGGCCCCATCTTTCTACTGGGAAATTGAGATTGTTTCCTATGGAGACACTGATGATGACACTGGACCAATCGTCTCATTTGGCTTCGCTACAGAAGCTGAGAAGCGAGATGGGGCATGGACCAATCCAGTGGGCACTTGCCTCTTCCACAACAATGGCCGAGCTGTACACTACAATGGCTCCAGTCTACTGCAGTGGAAGAGCGTCCGCTTAGATGTCACACTCTCTCCTGGCGATGTGGCAGGAATCGGCTGGGAGAGGACCGAGGGGACTCCACCTCCTCCAGGGCAGCCAGCCAAAGGTCGGGTGTACTTCACATACTGCGGGCAGCGCCTCACACCATACCTAGAAGATGTCTCTGGTGGCATGTGGCCTGTGGTTCACATTCAGAAAAAGAACACAAAAACTCGAGCCAATTTTGGCTCCCGCCCATTTGCGTATGCTGAAGGACAGGCCCACCGAAATGCTGCCGACCTGTGCACTGACCTGGCAGAAGAGATCAGCGCTAACTTTGAGGCCTTGCCATTCGCCATGGCATCTGACAGTGACAACGATGCTGGCACCAGTATTGCATCAGACCCAGGCACTCATGGGCCGCCATGCCGGATTGCAGCAGTGGCCACAGCCCAGCAGCAATATGACAGTGACACCTCCTGCCACTATAAAGTAGAGCTCAGCTATGAGAATTTCATTACCTCAGGCCCAGACCCCCACCCACCTCCCATTGCGGATGATGAGAGTGATGATGACGACGATGATGACATCCCTCAGGAGGACCACTACGCCCTGCTGGTGAAAGCATGGGAGACCAAGGTTTTCCCTACCATCCGGAGACGCTTCCGCAATGAAGCCGAGCGAAAGTCGGGCCTAGACCAGATCAAGGGTGCTTTACAACTAGGCATGGTGGACATCGCCCGGCAGACAGTTGAGTTCCTCTATGAGGAGAATGGCGGCATCCCCAGAGACCTTTATCTTCCTACCATTGAGGACATTAAAGATGAAGCAAACAAGTTCACAATCGACAAAGTTCGAAAAGGTCTCACGGTTGTCACCCGCTCTCCAGACAGCAGCAATGTAGCTAGTAGTACCGTGGGAACTGCTCTGCCAAAATTTGCCATCCGAGGGATGCTAAAAACCTTCAGCCTTCATGGAGTCGTCCTAGATGTTGATTCAGTGAATGAATTGGTGCAGGTGGAGACATACCTGCGGAGTGAAGGGGTGTTGGTGCGGTACTGGTACCCCATTGATATGCTAGAAAGGCCCCCTGCAGGCTACCGGAGGACTGCCACCAATGGCCTGGTCACACTGGACAATACCAACCTTCAAATTCACAGAGAGCTGCTGCGATGTGAGGCTGCACTGGCCCGACTGTACTGTCGCATGGCCCTGCTTAACATCTTTGCCCCCAAGCTACCTCATCTATTCACTCGGCTCTTCCACATCCCTGCCATCCGAGACATCACGTTAGAGCACCTGCAGCTCCTGTCCAATCAGCTCCTTGCTCCTCCCCTCCCAGATGGCACCATCAGCTCCAGCTCCATCCTCCTGGCACAGTCTCTGCAGCACTGCATCCACTCCCAGAGCTGCTCTGCCACCGACCTCTTCTACCAGGGCAGCTCGCAGACACTGAGGGAGTGGCTCAGCGTGGCCATCACCCGCACCCTGCACCAAGGCGAGGACAGCCTCCTGGAGCTGACAAAGCAGATCTGCGCCTTCCTGCAGACAGCACCAGAGCAGTTCCCCTCCGAGGAATTCCCAGTGTCTGAGTCCAAAGTCAGTATGGATGTGAATTTCCCTGGAGCAGCTTTTGTTGTTGTGTCTTGCAAAGAAAGTCAGTCTGGATTCCGCAAAGACTCCTCTTTGTACAAGGCTCCGTGGGCACGCGTGCTCGTCTATGGGCTTGGCCACAAAGTGAAGCGTACCGGCCAGCTGAACCTCATTGAGGCTGCCTGCTACCCTCGAGACGCCTCCCCAGCCAACACTGGGCTCGCCCCTCCACCCACAGCTGACCAGTACCCTTCTGTGGTTCTTTCCACAGACAAAGTCCACATCAAACTAGGAGTGTCCCCACCACCTGGAGCAGTGCTGGTCTTGCATTCTCTGCCCCTGGAGTTCCCGCTGGCGATGGCCTTTGCCGAGCAGCTGCTGTCCTGGAAATCAGAGGACAGTGATGGGAAGTCAGAGGATGAGCCTGACAGCATCCCTGCCTCCGTCCTCCTGCACGTGGTGGAACTGCTGGTAGCTCAGAAGGACGGCCCTTTCGACTCGGCACTGGTGCCAACATGGAAAAAGTTGTGA
Peptide sequence:
MEKVVKMDRDMTKGGCCEVITEEASAALRKATKWAQSGLIVSVGPPVESINPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKYAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPVDCRRTSQAADTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEMQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLTPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSSNVASSTVGTALPKFAIRGMLKTFSLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKLPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQSCSATDLFYQGSSQTLREWLSVAITRTLHQGEDSLLELTKQICAFLQTAPEQFPSEEFPVSESKVSMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDSDGKSEDEPDSIPASVLLHVVELLVAQKDGPFDSALVPTWKKL
IRESfinder result
IRESfinder score
0.441946587
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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