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mmu_circHeatr5b_493
Basic information of circular RNA
PanCircBase ID:
mmu_circHeatr5b_493
mm39_Chr. Position ID:
chr17|79074308|79076032|-
Gene name:
Heatr5b
Transcript:
ENSMUST00000097281.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
29,28
Exon/intron offsets:
0,1486
Genomic length:
1724
Exon/intron sizes in nt:
2,55,238
Flanking exon/introns:
chr17:79072884-79074308|chr17:79076032-79080966
Splice length:
493
Other database IDs:
circAtlas: mmu-Heatr5b_0024
CIRCpedia: HSA_CIRCpedia_122291
Human Ortholog IDs:
Mature spliced Sequence:
GTATATGTGGTTGCTATGAATATTAAGAAAGAAGCAGAGTCAAAACCAAAGAGAGCAATGAACAATCCTGATGATGATGATGACGACTATGGCACCATCGACGAGCTGCCTCCAGACAGCTTAATAACCCTGGTGCAGCCTGAGCTGCCGACGCTCAGCCGCCTGTGGTTGGCAGCACTGAAAGACTACGCGCTTTTGACTTTACCAGCTGAATTTTCTAGTCAGCTTCCCCCAGATGGGGGGGCATTTTATACTCCAGAGACCATCGATACAGCAAGACTTCACTACAGGAACTCCTGGGCCCCAATTCTCCATGCAGTGGCACTCTGGTTAAACAGCACAGGATTTATAAGTCAAGAGTCCACAGAAGCAACAACTGTGTCTGGTGTCCAGAAACGCTCCCCAGCTGTCAGTTTAAACCAAGTTCCAGGAGCAATGGCTAGTGCTAAACCCTTGCCAGAAGTTAACAAAGACAGGATGCACCTGATCCTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAAGTTCCAGGAGCAATGGC
59.47
CGATGGTGCCATAGTCGTCA
59.9
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGCTAAACCCTTGCCAGA
59.23
CGATGGTGCCATAGTCGTCA
59.9
152
PCR template sequence:
AAACGCTCCCCAGCTGTCAGTTTAAACCAAGTTCCAGGAGCAATGGCTAGTGCTAAACCCTTGCCAGAAGTTAACAAAGACAGGATGCACCTGATCCTAGGTATATGTGGTTGCTATGAATATTAAGAAAGAAGCAGAGTCAAAACCAAAGAGAGCAATGAACAATCCTGATGATGATGATGACGACTATGGCACCATCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-217-5p(1), mmu-miR-96-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CACCTGATCCTAGGTATATGTGGTTG
siRNA sets
CAACCACATATACCTAGGA, AACCACATATACCTAGGAT, CACATATACCTAGGATCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHEATR5B_493_ORF_1
0.7999
0.154775728
0.620386524
504
ORF sequence:
ATGAATATTAAGAAAGAAGCAGAGTCAAAACCAAAGAGAGCAATGAACAATCCTGATGATGATGATGACGACTATGGCACCATCGACGAGCTGCCTCCAGACAGCTTAATAACCCTGGTGCAGCCTGAGCTGCCGACGCTCAGCCGCCTGTGGTTGGCAGCACTGAAAGACTACGCGCTTTTGACTTTACCAGCTGAATTTTCTAGTCAGCTTCCCCCAGATGGGGGGGCATTTTATACTCCAGAGACCATCGATACAGCAAGACTTCACTACAGGAACTCCTGGGCCCCAATTCTCCATGCAGTGGCACTCTGGTTAAACAGCACAGGATTTATAAGTCAAGAGTCCACAGAAGCAACAACTGTGTCTGGTGTCCAGAAACGCTCCCCAGCTGTCAGTTTAAACCAAGTTCCAGGAGCAATGGCTAGTGCTAAACCCTTGCCAGAAGTTAACAAAGACAGGATGCACCTGATCCTAGGTATATGTGGTTGCTATGAATATTAA
Peptide sequence:
MNIKKEAESKPKRAMNNPDDDDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFISQESTEATTVSGVQKRSPAVSLNQVPGAMASAKPLPEVNKDRMHLILGICGCYEY
IRESfinder result
IRESfinder score
0.472101036
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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