top of page
mmu_circHap1_767
Basic information of circular RNA
PanCircBase ID:
mmu_circHap1_767
mm39_Chr. Position ID:
chr11|100242035|100244961|-
Gene name:
Hap1
Transcript:
ENSMUST00000138603.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
10,9,8,7,6,5,4
Exon/intron offsets:
0,208,633,975,2334,2478,2747
Genomic length:
2926
Exon/intron sizes in nt:
9,21,35,63,13,16,71,00,000
Flanking exon/introns:
chr11:100240445-100242035|chr11:100244961-100245071
Splice length:
767
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGACCTTCTTCAGCTCTACTCAGACTCTGATGACGATGATGATGAGGAAGACGAGGAAGACGAGGAAGAGGGCGAAGAGGAGGAACGAGAAGGACAGAGGGATCAAGACCAGCAGCACGACCACCCCTATGGTGCCCCCAAGCCACACCCTAAGGCTGAGACAGCGCACCGCTGCCCACAGCTGGAAACCCTGCAGCAGAAGCTCAGGCTTCTGGAGGAAGAGAACGACCACCTGCGAGAGGAGGCCTCCCACCTTGACAACCTGGAGGACGAAGAGCAGATGCTCATTCTGGAATGTGTGGAGCAGTTCTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCGGAAGTGCTGGTGTTGAGGCTGGAAGGCTATGAGAGGCAGCAGAAAGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAGCGTTGTCAGTCTTATGGGGCCCAGACAGAGAAACTGCAGCAGATGCTGGCCTCAGAGAAGGGGATCCACTCGGAGAGCCTGCGAGCTGGCTCCTACATGCAGGATTATGGGAGCAGGCCTCGTGACCGCCAGGAGGATGGGAAGAGTCATCGCCAGCGCTCCTCCATGCCCGCAGGCTCTGTCACCCACTATGGATACAGTGTGCCTCTGGATGCACTTCCAAGTTTCCCAGAGACACTGGCTGAGGAGCTCCGAACATCTCTGAGGAAGTTCATCACTGACCCTGCGTATTTCATGGAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTCTGGATGCACTTCCAA
60.32
AGGTCATCTCGCAGGTTCAC
59.75
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGAGGAGCTCCGAACATC
60.53
CCTCGTCTTCCTCGTCTTCC
59.55
162
PCR template sequence:
TGCCTCTGGATGCACTTCCAAGTTTCCCAGAGACACTGGCTGAGGAGCTCCGAACATCTCTGAGGAAGTTCATCACTGACCCTGCGTATTTCATGGAGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGACCTTCTTCAGCTCTACTCAGACTCTGATGACGATGATGATGAGGAAGACGAGGAAGACGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-146a-5p(1), mmu-miR-499-5p(1), mmu-miR-1904(1), mmu-miR-146b-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTCATGGAGAGATTTTACATCTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHAP1_767_ORF_1
1.0852
0.413389213
0.86662828
477
ORF sequence:
ATGCTCATTCTGGAATGTGTGGAGCAGTTCTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCGGAAGTGCTGGTGTTGAGGCTGGAAGGCTATGAGAGGCAGCAGAAAGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAGCGTTGTCAGTCTTATGGGGCCCAGACAGAGAAACTGCAGCAGATGCTGGCCTCAGAGAAGGGGATCCACTCGGAGAGCCTGCGAGCTGGCTCCTACATGCAGGATTATGGGAGCAGGCCTCGTGACCGCCAGGAGGATGGGAAGAGTCATCGCCAGCGCTCCTCCATGCCCGCAGGCTCTGTCACCCACTATGGATACAGTGTGCCTCTGGATGCACTTCCAAGTTTCCCAGAGACACTGGCTGAGGAGCTCCGAACATCTCTGAGGAAGTTCATCACTGACCCTGCGTATTTCATGGAGAGATTTTACATCTCCGGAAGCAGGTGA
Peptide sequence:
MLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERFYISGSR
IRESfinder result
IRESfinder score
0.214248235
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page