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mmu_circHace1_597
Basic information of circular RNA
PanCircBase ID:
mmu_circHace1_597
mm39_Chr. Position ID:
chr10|45481604|45526083|+
Gene name:
Hace1
Transcript:
ENSMUST00000037044.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
5,6,7,8,9,10
Exon/intron offsets:
0,12989,32812,43082,43345,44372
Genomic length:
44479
Exon/intron sizes in nt:
76,13,28,39,71,02,107
Flanking exon/introns:
chr10:45466468-45481604|chr10:45526083-45528683
Splice length:
597
Other database IDs:
CIRCpedia: MMU_CIRCpedia_36566, circAtlas: mmu-Hace1_0039
circBase: hsa_circ_0130159, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_99964
Human Ortholog IDs:
Mature spliced Sequence:
ACAGAAGAAATGCATGAGTAAATTACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTGAATGGGAGGACAGAGCTCCTCCATGACCTCGTGCAACACGTCACAGATGTGGATGTGGAGGATGCCATGGGGCAGACAGCGCTACACGTAGCCTGCCAGAACGGGCACAAAACGACAGTGCAGTGCTTGCTAGACAGCGGTGCTGATATTAACAGGCCGAATGTATCAGGAGCCACGCCACTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACTGCACAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAATGGAGTAACCCCTCTAGATCTATGTGTACAGGGTGGTTACGGACAGACTTGTGAAGTGTTAATTCAGTACCACCCGAGGCTTTTCCAGACTATTGTTCAAATGACACAGAATGAAGACCTTCGGGAAAACATGTTACGGCAAGTTCTGCAGCATTTGTCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCCATAAGCTGCTTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGATCCTGACAGGCCTTGC
60.03
CAGCCAGCCAGTGTATTGCT
60.68
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGATCCTGACAGGCCTTGC
60.03
TCACAGCCAGCCAGTGTATT
59.31
151
PCR template sequence:
AAGTTCTGCAGCATTTGTCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCCATAAGCTGCTTAGACAGAAGAAATGCATGAGTAAATTACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTGAATGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-10b-5p(1), mmu-miR-139-5p(1), mmu-miR-10a-5p(1), mmu-miR-136-5p(1), mmu-miR-382-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATAAGCTGCTTAGACAGAAGAAATGC
siRNA sets
TTTCTTCTGTCTAAGCAGC, CATTTCTTCTGTCTAAGCA, TTCTTCTGTCTAAGCAGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHACE1_597_ORF_1
1.0863
0.129348257
0.996939219
1179
ORF sequence:
ATGAGTAAATTACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTGAATGGGAGGACAGAGCTCCTCCATGACCTCGTGCAACACGTCACAGATGTGGATGTGGAGGATGCCATGGGGCAGACAGCGCTACACGTAGCCTGCCAGAACGGGCACAAAACGACAGTGCAGTGCTTGCTAGACAGCGGTGCTGATATTAACAGGCCGAATGTATCAGGAGCCACGCCACTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACTGCACAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAATGGAGTAACCCCTCTAGATCTATGTGTACAGGGTGGTTACGGACAGACTTGTGAAGTGTTAATTCAGTACCACCCGAGGCTTTTCCAGACTATTGTTCAAATGACACAGAATGAAGACCTTCGGGAAAACATGTTACGGCAAGTTCTGCAGCATTTGTCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCCATAAGCTGCTTAGACAGAAGAAATGCATGAGTAAATTACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTGAATGGGAGGACAGAGCTCCTCCATGACCTCGTGCAACACGTCACAGATGTGGATGTGGAGGATGCCATGGGGCAGACAGCGCTACACGTAGCCTGCCAGAACGGGCACAAAACGACAGTGCAGTGCTTGCTAGACAGCGGTGCTGATATTAACAGGCCGAATGTATCAGGAGCCACGCCACTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACTGCACAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAATGGAGTAACCCCTCTAGATCTATGTGTACAGGGTGGTTACGGACAGACTTGTGAAGTGTTAATTCAGTACCACCCGAGGCTTTTCCAGACTATTGTTCAAATGACACAGAATGAAGACCTTCGGGAAAACATGTTACGGCAAGTTCTGCAGCATTTGTCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCCATAAGCTGCTT
Peptide sequence:
MSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLRQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLL
IRESfinder result
IRESfinder score
0.314591515
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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