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mmu_circGucy2c_452
Basic information of circular RNA
PanCircBase ID:
mmu_circGucy2c_452
mm39_Chr. Position ID:
chr6|136720913|136737769|-
Gene name:
Gucy2c
Transcript:
ENSMUST00000032338.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
10,9,8,7
Exon/intron offsets:
0,4863,7003,16738
Genomic length:
16856
Exon/intron sizes in nt:
11,28,61,36,118
Flanking exon/introns:
chr6:136717689-136720913|chr6:136737769-136739995
Splice length:
452
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TAACCATTACTTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGTCCACCTCAAACACCTCTGTCGCCGAGAGGTTTTCATCGGGGAGAAGTGACTTTTCTCTCGCTTACTTGGAGGGAACCTTGCTATTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAAAATGTCACGGGTCCCAAGTTTGCTCGTGCATTCAGGAATCTCACTTTTCAAGGCTTTGCAGGACCTGTGACTCTGGATGACAGTGGGGACATTGACAACATTATGTCCCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGAAGTATGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTCCCCAATGACGTTCCTGGGCTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTGAGAACCCCAACTTCA
59.89
GAGGTGGACTGTTCAGACGG
60.04
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCTGAGAACCCCAACTTC
59.89
GAGGTGGACTGTTCAGACGG
60.04
150
PCR template sequence:
ATGAAGTATGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTCCCCAATGACGTTCCTGGGCTGGTAACCATTACTTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGTCCACCTCAAACACCTCTGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTCCTGGGCTGGTAACCATTACTTT
siRNA sets
AAGTAATGGTTACCAGCCC, AATGGTTACCAGCCCAGGA, TAATGGTTACCAGCCCAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGUCY2C_452_ORF_1
1.1204
0.166049285
0.714787689
474
ORF sequence:
ATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGTCCACCTCAAACACCTCTGTCGCCGAGAGGTTTTCATCGGGGAGAAGTGACTTTTCTCTCGCTTACTTGGAGGGAACCTTGCTATTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAAAATGTCACGGGTCCCAAGTTTGCTCGTGCATTCAGGAATCTCACTTTTCAAGGCTTTGCAGGACCTGTGACTCTGGATGACAGTGGGGACATTGACAACATTATGTCCCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGAAGTATGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTCCCCAATGACGTTCCTGGGCTGGTAACCATTACTTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGA
Peptide sequence:
MDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGHMLQTFLENGENVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYKVLMKYDTHKNKTIPVAENPNFIWKNHKLPNDVPGLVTITLRRTPQLLSIWTMSSS
IRESfinder result
IRESfinder score
0.596253899
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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