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mmu_circGspt1_904_1
Basic information of circular RNA
PanCircBase ID:
mmu_circGspt1_904_1
mm39_Chr. Position ID:
chr16|11040448|11048855|-
Gene name:
Gspt1
Transcript:
ENSMUST00000167571.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
14,13,12,11,10,9,8
Exon/intron offsets:
0,627,1835,5530,6624,7925,8252
Genomic length:
8407
Exon/intron sizes in nt:
1,69,90,17,48,19,41,41,000
Flanking exon/introns:
chr16:11038743-11040448|chr16:11048855-11050395
Splice length:
904
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAATCTCAGCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTCAAACACTTAATTGTGCTTATTAATAAGATGGATGATCCAACGGTAAACTGGAGCAACGAGAGATATGAAGAATGTAAAGAGAAACTAGTACCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCATTTTATGCCGTGCTCAGGACTGACAGGAGCGAATCTTAAAGAACAATCAGATTTCTGTCCTTGGTACATTGGCTTACCATTTATTCCATATCTGGATAATTTGCCAAACTTCAATAGATCAGTTGATGGACCAATCAGGCTGCCAATTGTCGATAAGTACAAGGATATGGGGACTGTGGTCCTGGGAAAGCTGGAATCTGGATCTATTTGTAAAGGCCAGCAGCTTGTAATGATGCCAAATAAGCACAACGTGGAAGTTCTTGGAATACTTTCTGATGATGTAGAAACGGACTCTGTAGCTCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATTGAAGAGGAAGAGATTCTTCCAGGATTCATCCTTTGTGATCTTAATAATCTTTGTCATTCTGGACGCACATTTGATGCTCAGATAGTGATTATAGAGCACAAGTCCATCATCTGCCCAGGGTATAATGCGGTGCTGCATATTCATACCTGTATCGAAGAAGTCGAAATAACAGCCTTAATCTGCTTGGTAGACAAGAAATCAGGAGAGAAAAGTAAGACTCGACCCCGTTTTGTAAAACAAGATCAAGTATGCATTGCGCGTTTAAGGACAGCAGGAACCATCTGCCTGGAGACCTTTAAGGACTTCCCTCAGATGGGACGTTTTACCTTAAGAGATGAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGAACCATCTGCCTGGAG
60.11
CACCTGCTGTCTTTGCCAAC
59.97
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAACCATCTGCCTGGAGACC
60.11
CACCTGCTGTCTTTGCCAAC
59.97
157
PCR template sequence:
GATCAAGTATGCATTGCGCGTTTAAGGACAGCAGGAACCATCTGCCTGGAGACCTTTAAGGACTTCCCTCAGATGGGACGTTTTACCTTAAGAGATGAGGGTAATCTCAGCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTCAAACACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-184-3p(1), mmu-miR-370-3p(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTAAGAGATGAGGGTAATCTCAGCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGSPT1_904_1_ORF_1
0.9616
0.069441238
0.965987582
909
ORF sequence:
ATGAGGGTAATCTCAGCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTCAAACACTTAATTGTGCTTATTAATAAGATGGATGATCCAACGGTAAACTGGAGCAACGAGAGATATGAAGAATGTAAAGAGAAACTAGTACCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCATTTTATGCCGTGCTCAGGACTGACAGGAGCGAATCTTAAAGAACAATCAGATTTCTGTCCTTGGTACATTGGCTTACCATTTATTCCATATCTGGATAATTTGCCAAACTTCAATAGATCAGTTGATGGACCAATCAGGCTGCCAATTGTCGATAAGTACAAGGATATGGGGACTGTGGTCCTGGGAAAGCTGGAATCTGGATCTATTTGTAAAGGCCAGCAGCTTGTAATGATGCCAAATAAGCACAACGTGGAAGTTCTTGGAATACTTTCTGATGATGTAGAAACGGACTCTGTAGCTCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATTGAAGAGGAAGAGATTCTTCCAGGATTCATCCTTTGTGATCTTAATAATCTTTGTCATTCTGGACGCACATTTGATGCTCAGATAGTGATTATAGAGCACAAGTCCATCATCTGCCCAGGGTATAATGCGGTGCTGCATATTCATACCTGTATCGAAGAAGTCGAAATAACAGCCTTAATCTGCTTGGTAGACAAGAAATCAGGAGAGAAAAGTAAGACTCGACCCCGTTTTGTAAAACAAGATCAAGTATGCATTGCGCGTTTAAGGACAGCAGGAACCATCTGCCTGGAGACCTTTAAGGACTTCCCTCAGATGGGACGTTTTACCTTAAGAGATGAG
Peptide sequence:
MRVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGFILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDE
IRESfinder result
IRESfinder score
0.647781694
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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