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mmu_circGrb2_292
Basic information of circular RNA
PanCircBase ID:
mmu_circGrb2_292
mm39_Chr. Position ID:
chr11|115537535|115540719|-
Gene name:
Grb2
Transcript:
ENSMUST00000106497.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,3
Exon/intron offsets:
0,3061
Genomic length:
3184
Exon/intron sizes in nt:
1,69,123
Flanking exon/introns:
chr11:115536780-115537535|chr11:115540719-115546183
Splice length:
292
Other database IDs:
circBase: hsa_circ_0107650, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_22360
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTTTTTTGGCAAAATCCCCAGAGCCAAGGCAGAAGAAATGCTCAGCAAACAGCGGCATGACGGGGCCTTCCTGATCCGAGAGAGCGAGAGCGCTCCTGGGGACTTCTCCCTGTCCGTCAAGTTTGGAAATGATGTGCAGCACTTCAAGGTGCTCCGCGACGGAGCCGGGAAGTATTTCCTGTGGGTGGTGAAGTTTAATTCTTTGAATGAGCTGGTAGATTACCACAGATCAACATCCGTGTCCAGGAACCAGCAGATATTCTTACGGGACATAGAACAGATGCCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACATCCGTGTCCAGGAACC
59.96
TCGCTCTCTCGGATCAGGAA
60.11
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACATCCGTGTCCAGGAACC
59.96
CGCTCTCTCGGATCAGGAAG
59.69
147
PCR template sequence:
GAAGTTTAATTCTTTGAATGAGCTGGTAGATTACCACAGATCAACATCCGTGTCCAGGAACCAGCAGATATTCTTACGGGACATAGAACAGATGCCACAGGTGGTTTTTTGGCAAAATCCCCAGAGCCAAGGCAGAAGAAATGCTCAGCAAACAGCGGCATGACGGGGCCTTCCTGATCCGAGAGAGCGAGAGCGCTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
ACAGATGCCACAGGTGGTTTTTTGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGRB2_292_ORF_1
1.1238
0.260526207
0.542751541
315
ORF sequence:
ATGCTCAGCAAACAGCGGCATGACGGGGCCTTCCTGATCCGAGAGAGCGAGAGCGCTCCTGGGGACTTCTCCCTGTCCGTCAAGTTTGGAAATGATGTGCAGCACTTCAAGGTGCTCCGCGACGGAGCCGGGAAGTATTTCCTGTGGGTGGTGAAGTTTAATTCTTTGAATGAGCTGGTAGATTACCACAGATCAACATCCGTGTCCAGGAACCAGCAGATATTCTTACGGGACATAGAACAGATGCCACAGGTGGTTTTTTGGCAAAATCCCCAGAGCCAAGGCAGAAGAAATGCTCAGCAAACAGCGGCATGA
Peptide sequence:
MLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQVVFWQNPQSQGRRNAQQTAA
IRESfinder result
IRESfinder score
0.440735991
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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