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mmu_circGrb14_699
Basic information of circular RNA
PanCircBase ID:
mmu_circGrb14_699
mm39_Chr. Position ID:
chr2|64760243|64784062|-
Gene name:
Grb14
Transcript:
ENSMUST00000028252.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
8,7,6,5,4,3
Exon/intron offsets:
0,286,3320,6517,8469,23662
Genomic length:
23819
Exon/intron sizes in nt:
9,61,11,13,87,51,22,150
Flanking exon/introns:
chr2:64753961-64760243|chr2:64784062-64851430
Splice length:
699
Other database IDs:
circBase: hsa_circ_0117885, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_122690
Human Ortholog IDs:
Mature spliced Sequence:
GTGATTAAAGTTTACAGCGAGGATGAAACCAGCAGAGCATTAGAGGTGCCCAGTGACATCACAGCCCGAGATGTTTGCCAGCTGTTGATCCTGAAGAACCACTATGTGGACGACAACAGCTGGACCCTTTTTGAGCACCTATCTCACATAGGTTTAGAAAGAACCGTAGAGGACCACGAGCTGCCAACTGAAGTGCTGTCTCACTGGGGAGTGGAAGAAGACAATAAGCTGTATCTTAGAAAGAATTATGCCAAATATGAATTTTTTAAGAACCCAATGTATTTCTTTCCAGAGCACATGGTGTCTTTTGCAGCTGAAATGAATGGTGACAGATCCCCTACACAGATACTGCAGGTGTTTTTAAGCTCCAGCACGTATCCTGAAATCCATGGCTTCTTACATGCAAAGGAACAGGGAAAGAAGTCTTGGAAAAAAGCTTACTTTTTTCTCAGAAGATCTGGCTTATATTTTTCTACTAAAGGCACATCCAAGGAACCACGGCATTTGCAGCTTTTCAGTGAATTCAGCACTAGTCACGTTTATATGTCACTGGCAGGAAAAAAAAAACACGGAGCGCCAACTCCCTATGGATTCTGCTTAAAGCCTAACAAAGCAGGAGGGCCCCGGGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGCAGGACGTGCTGGGTGACCGCCATCCGACTGCTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGGGACCTGAAAATGCTCT
60.04
CATCTCGGGCTGTGATGTCA
59.82
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGGGACCTGAAAATGCTCT
60.04
ATCTCGGGCTGTGATGTCAC
59.82
148
PCR template sequence:
AAAGCCTAACAAAGCAGGAGGGCCCCGGGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGCAGGACGTGCTGGGTGACCGCCATCCGACTGCTGAAGGTGATTAAAGTTTACAGCGAGGATGAAACCAGCAGAGCATTAGAGGTGCCCAGTGACATCACAGCCCGAGATGTTTGCCAGCTGTTGATCCTGAAGAACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-30d-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCGACTGCTGAAGGTGATTAAAGTTT
siRNA sets
AAACTTTAATCACCTTCAG, TTTAATCACCTTCAGCAGT, AACTTTAATCACCTTCAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGRB14_699_ORF_1
0.6354
0.074484395
0.986705973
1122
ORF sequence:
ATGTATTTCTTTCCAGAGCACATGGTGTCTTTTGCAGCTGAAATGAATGGTGACAGATCCCCTACACAGATACTGCAGGTGTTTTTAAGCTCCAGCACGTATCCTGAAATCCATGGCTTCTTACATGCAAAGGAACAGGGAAAGAAGTCTTGGAAAAAAGCTTACTTTTTTCTCAGAAGATCTGGCTTATATTTTTCTACTAAAGGCACATCCAAGGAACCACGGCATTTGCAGCTTTTCAGTGAATTCAGCACTAGTCACGTTTATATGTCACTGGCAGGAAAAAAAAAACACGGAGCGCCAACTCCCTATGGATTCTGCTTAAAGCCTAACAAAGCAGGAGGGCCCCGGGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGCAGGACGTGCTGGGTGACCGCCATCCGACTGCTGAAGGTGATTAAAGTTTACAGCGAGGATGAAACCAGCAGAGCATTAGAGGTGCCCAGTGACATCACAGCCCGAGATGTTTGCCAGCTGTTGATCCTGAAGAACCACTATGTGGACGACAACAGCTGGACCCTTTTTGAGCACCTATCTCACATAGGTTTAGAAAGAACCGTAGAGGACCACGAGCTGCCAACTGAAGTGCTGTCTCACTGGGGAGTGGAAGAAGACAATAAGCTGTATCTTAGAAAGAATTATGCCAAATATGAATTTTTTAAGAACCCAATGTATTTCTTTCCAGAGCACATGGTGTCTTTTGCAGCTGAAATGAATGGTGACAGATCCCCTACACAGATACTGCAGGTGTTTTTAAGCTCCAGCACGTATCCTGAAATCCATGGCTTCTTACATGCAAAGGAACAGGGAAAGAAGTCTTGGAAAAAAGCTTACTTTTTTCTCAGAAGATCTGGCTTATATTTTTCTACTAAAGGCACATCCAAGGAACCACGGCATTTGCAGCTTTTCAGTGAATTCAGCACTAGTCACGTTTATATGTCACTGGCAGGAAAAAAAAAACACGGAGCGCCAACTCCCTATGGATTCTGCTTAAAGCCTAACAAAGCAGGAGGGCCCCGGGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGCAGGACGTGCTGGGTGACCGCCATCCGACTGCTGAAG
Peptide sequence:
MYFFPEHMVSFAAEMNGDRSPTQILQVFLSSSTYPEIHGFLHAKEQGKKSWKKAYFFLRRSGLYFSTKGTSKEPRHLQLFSEFSTSHVYMSLAGKKKHGAPTPYGFCLKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYVDDNSWTLFEHLSHIGLERTVEDHELPTEVLSHWGVEEDNKLYLRKNYAKYEFFKNPMYFFPEHMVSFAAEMNGDRSPTQILQVFLSSSTYPEIHGFLHAKEQGKKSWKKAYFFLRRSGLYFSTKGTSKEPRHLQLFSEFSTSHVYMSLAGKKKHGAPTPYGFCLKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLK
IRESfinder result
IRESfinder score
0.591532541
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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