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mmu_circGrb14_359
Basic information of circular RNA
PanCircBase ID:
mmu_circGrb14_359
mm39_Chr. Position ID:
chr2|64747172|64753961|-
Gene name:
Grb14
Transcript:
ENSMUST00000028252.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
12,11,10,9
Exon/intron offsets:
0,206,370,6708
Genomic length:
6789
Exon/intron sizes in nt:
88,73,117,81
Flanking exon/introns:
chr2:64745153-64747172|chr2:64753961-64760243
Splice length:
359
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATGGCATGCAGCTGTATCAGAATTATATGCATCCATACCAAGGTAGAAGCGCCTGCAATTCTCAGAGCATGTCACCCATGAGAAGCGTATCAGAGAATTCCCTAGTAGCAATGGACTTCTCAGGTGAGAAGAGCAGAGTCATAGACAACCCCACTGAAGCGCTTTCGGTTGCTGTTGAGGAAGGCCTCGCGTGGAGGAAAAAAGGCTGTTTACGCCTGGGGAATCACGGAAGCCCCAGTGCCCCCTCCCAGAGCTCTGCTGTGAACATGGCTCTCCATCGGTCCCAACCATGGTTTCACCACAGAATTTCCAGAGATGAGGCTCAGCGGCTGATCATTCGGCAGGGGCCTGTGGATGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGGTCCCAACCATGGTTTC
60.25
ATTGCAGGCGCTTCTACCTT
60.04
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGGTCCCAACCATGGTTTC
60.25
AATTGCAGGCGCTTCTACCT
60.04
142
PCR template sequence:
CTGTGAACATGGCTCTCCATCGGTCCCAACCATGGTTTCACCACAGAATTTCCAGAGATGAGGCTCAGCGGCTGATCATTCGGCAGGGGCCTGTGGATGGGATGGCATGCAGCTGTATCAGAATTATATGCATCCATACCAAGGTAGAAGCGCCTGCAATTCTCAGAGCATGTCACCCATGAGAAGCGTATCAGAGAATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-17-5p(1), mmu-miR-30e-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-30b-5p(1), mmu-miR-351-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCCTGTGGATGGGATGGCATGCAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGRB14_359_ORF_1
0.6588
0.04359701
0.327957053
435
ORF sequence:
ATGCAGCTGTATCAGAATTATATGCATCCATACCAAGGTAGAAGCGCCTGCAATTCTCAGAGCATGTCACCCATGAGAAGCGTATCAGAGAATTCCCTAGTAGCAATGGACTTCTCAGGTGAGAAGAGCAGAGTCATAGACAACCCCACTGAAGCGCTTTCGGTTGCTGTTGAGGAAGGCCTCGCGTGGAGGAAAAAAGGCTGTTTACGCCTGGGGAATCACGGAAGCCCCAGTGCCCCCTCCCAGAGCTCTGCTGTGAACATGGCTCTCCATCGGTCCCAACCATGGTTTCACCACAGAATTTCCAGAGATGAGGCTCAGCGGCTGATCATTCGGCAGGGGCCTGTGGATGGGATGGCATGCAGCTGTATCAGAATTATATGCATCCATACCAAGGTAGAAGCGCCTGCAATTCTCAGAGCATGTCACCCATGA
Peptide sequence:
MQLYQNYMHPYQGRSACNSQSMSPMRSVSENSLVAMDFSGEKSRVIDNPTEALSVAVEEGLAWRKKGCLRLGNHGSPSAPSQSSAVNMALHRSQPWFHHRISRDEAQRLIIRQGPVDGMACSCIRIICIHTKVEAPAILRACHP
IRESfinder result
IRESfinder score
0.270237163
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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