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mmu_circGpr158_110
Basic information of circular RNA
PanCircBase ID:
mmu_circGpr158_110
mm39_Chr. Position ID:
chr2|21751486|21751596|+
Gene name:
Gpr158
Transcript:
ENSMUST00000055946.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
110
Exon/intron sizes in nt:
110
Flanking exon/introns:
chr2:21653836-21751486|chr2:21751596-21787874
Splice length:
110
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCGTTATTCTGTACTTTGAGCCAAGTACATTTCGTTGTATTCTCCTAAGGTGGGCTCGTCTTCTTGGTTTTGCTACTGTTTATGGAACTGTGACCCTCAAGCTTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCAAGCTTCACAGGTCGT
59.97
CCCACCTTAGGAGAATACAACGA
59.55
70
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAACTGTGACCCTCAAGC
59.89
CCCACCTTAGGAGAATACAACGA
59.55
82
PCR template sequence:
CTCGTCTTCTTGGTTTTGCTACTGTTTATGGAACTGTGACCCTCAAGCTTCACAGGTCGTTATTCTGTACTTTGAGCCAAGTACATTTCGTTGTATTCTCCTAAGGTGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-127-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAAGCTTCACAGGTCGTTATTCTGT
siRNA sets
AATAACGACCTGTGAAGCT, ATAACGACCTGTGAAGCTT, GAATAACGACCTGTGAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.886290242
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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