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mmu_circGpr107_493
Basic information of circular RNA
PanCircBase ID:
mmu_circGpr107_493
mm39_Chr. Position ID:
chr2|31073591|31097740|+
Gene name:
Gpr107
Transcript:
ENSMUST00000056433.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
10,11,12,13,14,15
Exon/intron offsets:
0,1218,1909,5103,8267,24099
Genomic length:
24149
Exon/intron sizes in nt:
76,74,118,131,44,50
Flanking exon/introns:
chr2:31068399-31073591|chr2:31097740-31099078
Splice length:
493
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAATGATGTATTTAAAATTCACTGGTTGATGGCCGCCCTTCCTTTCACCAAGTCTCTCTCGTTGGTGTTCCATGCAATCGACTACCACTACATCTCCTCGCAGGGCTTTCCGATTGAAGGCTGGGCTGTTGTATACTACATAACTCACCTGCTAAAGGGCGCACTCCTGTTCATCACCATCGCCCTCATCGGCACTGGCTGGGCCTTCATCAAGCACATCCTGTCTGATAAAGACAAGAAGATCTTCATGATTGTCATCCCGCTCCAGGTCCTGGCGAATGTGGCCTACATCATCATAGAGTCCACTGAGGAGGGCACAACTGAGTATGGCCTATGGAAGGACTCTCTGTTCCTGGTTGACTTGCTTTGCTGTGGCGCCATCCTCTTCCCAGTGGTGTGGTCAATCAGGCATTTACAAGAAGCCTCCGCCACAGATGGAAAAGCTGCCATTAACTTAGCGAAGCTGAGGCTTTTCAGACATTACTACGTCTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTACAAGAAGCCTCCGCCAC
60.32
TGGAACACCAACGAGAGAGAC
59.66
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTACAAGAAGCCTCCGCCAC
60.32
ACCAACGAGAGAGACTTGGTG
59.66
147
PCR template sequence:
GGTGTGGTCAATCAGGCATTTACAAGAAGCCTCCGCCACAGATGGAAAAGCTGCCATTAACTTAGCGAAGCTGAGGCTTTTCAGACATTACTACGTCTTGGAATGATGTATTTAAAATTCACTGGTTGATGGCCGCCCTTCCTTTCACCAAGTCTCTCTCGTTGGTGTTCCATGCAATCGACTACCACTACATCTCCTCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-let-7c-5p(1), mmu-miR-218-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACTACGTCTTGGAATGATGTATTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGPR107_493_ORF_1
1.2089
0.33068191
0.849779833
471
ORF sequence:
ATGGCCGCCCTTCCTTTCACCAAGTCTCTCTCGTTGGTGTTCCATGCAATCGACTACCACTACATCTCCTCGCAGGGCTTTCCGATTGAAGGCTGGGCTGTTGTATACTACATAACTCACCTGCTAAAGGGCGCACTCCTGTTCATCACCATCGCCCTCATCGGCACTGGCTGGGCCTTCATCAAGCACATCCTGTCTGATAAAGACAAGAAGATCTTCATGATTGTCATCCCGCTCCAGGTCCTGGCGAATGTGGCCTACATCATCATAGAGTCCACTGAGGAGGGCACAACTGAGTATGGCCTATGGAAGGACTCTCTGTTCCTGGTTGACTTGCTTTGCTGTGGCGCCATCCTCTTCCCAGTGGTGTGGTCAATCAGGCATTTACAAGAAGCCTCCGCCACAGATGGAAAAGCTGCCATTAACTTAGCGAAGCTGAGGCTTTTCAGACATTACTACGTCTTGGAATGA
Peptide sequence:
MAALPFTKSLSLVFHAIDYHYISSQGFPIEGWAVVYYITHLLKGALLFITIALIGTGWAFIKHILSDKDKKIFMIVIPLQVLANVAYIIIESTEEGTTEYGLWKDSLFLVDLLCCGAILFPVVWSIRHLQEASATDGKAAINLAKLRLFRHYYVLE
IRESfinder result
IRESfinder score
0.654508125
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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