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mmu_circGolga1_1584
Basic information of circular RNA
PanCircBase ID:
mmu_circGolga1_1584
mm39_Chr. Position ID:
chr2|38923168|38954735|-
Gene name:
Golga1
Transcript:
ENSMUST00000039165.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,904,2068,5883,8070,13866,14440,15153,17002,18847,19799,22903,30085,31518
Genomic length:
31567
Exon/intron sizes in nt:
96,150,96,96,126,112,170,129,33,98,75,91,263,49
Flanking exon/introns:
chr2:38914310-38923168|chr2:38954735-38955392
Splice length:
1584
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGCAGTGACTTTGACCTCCATACTCTTTTAAATATAGTAGATAAGACAGATCTTGTGTTGCCAAAATTCTTTGGCCCACATTTAAGTGGGATGCAGCTAGCCTGGAGGTGTGAATGAATTTCATTCACTGAGAAGTCTAGGGGTTAGTTAACCCTGAAAGATATCCACACAGCAAAAATGTTTGCAAAACTAAAGAAGAAAATTGCAGAAGAGACTGCTGTTGCTCAGAGACCAGGCGGCACAACAAGGATCCCACGCTCTGTGAGCAAGGAGTCTGTGGCCTCCATGGGAGCTGACTCTGGAGATGATTTTGCTTCCGATGGTAGCAGCTCCAGAGAAGATCTCTCATCCCAGCTTTTGAGAAGAAATGAACAGATACGGAAGTTAGAGGCCAGACTTTCTGACTATGCTGAACAGGTCCGAAACTTGCAGAAGATAAAAGAGAAGCTTGAAATTGCATTAGAAAAACACCAGGATTCTTCCATGCGGAAATTTCAAGAACAGAATGAAACATTCCAAGCCAGCAGAGCCAAAATGGCAGAAGGACTGGCTTTGGCTTTAGCCAGGAAGGACCAGGAATGGTCAGAAAAGATGGAACAGCTTGAAAAGGATAAAAGGTTTCTAACATCTCAGCTGCAAGAAGTAAAGAACCAGAGCTTGAGCCTTTTTCAAAAAAGAGATGAAATTGATGAATTAGAGGGGTTCCAGCAGCAGGAAATCAGCAAAGTAAAACACATGCTTTTAAAAAAAGAAGAATGCTTGGGGAAGATGGAGCAAGAATTGGATGCACGAACCAGGGAACTTAACCGTACTCAGGAGGAGTTGGTGACTTCTAATCAGTTGTCATCTGACTTAAACGAGCGGCTAGAAGAATTGCAGAGACACTGCTCAACGCTGGAAGAGCAGAGAGATCACTTGACAGCTTCAAAAGCAGGTGCAGAACATAAGATTGTAGTCCTGGAACAAAAGGAACAAGAGCTCCAGGCGATCATCCAGCAGCATTCCATTGACCTACAAAAGGTCACTGCTGAAACGCAAGAAAAAGAAAAAGTCATCACACATCTACAAGAGAAGGTTATATTCTTGGAAAAGAGACTAGAACAGAATTTATCAGGAGAAGATCATGTACAAGAACTCTTGAAAGAGAAAACAGTTGCTGAACAGAATCTGGAAGATACTAGGCAGCAGCTCTTGGCTGCCAGGAACAGCCACACCAAGGCACTTTACCTCCTGGAGACTCGGGTGAAGGACCTAGAGCGGAGTCTACAGGCTGCTGAAGAACAGCTAAGCCAGAGTAGAAATGTTGTGGCTGACCAGGAAGCCCAGATCCAGAAGCTTATCACTACAAACCAGGAGAACAGCCTTTCCCAGCAGCAGGTTCTTGCCCTGGAACAGCATTGCAGGGAGCGCATCCATGCACTGGAGGCCCAAATTGAAGCTCTGGAACAAACACGGGTAGCTGACCAGATAGCCTCAGAGCAGGGAATGCTACAACTGCAGCAGGAAAATGTGGCCCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTACAACTGCAGCAGGAA
59.89
TGGCAACACAAGATCTGTCT
57.06
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGGAAAATGTGGCCCTT
60.18
TGGCAACACAAGATCTGTCT
57.06
149
PCR template sequence:
AATGCTACAACTGCAGCAGGAAAATGTGGCCCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAAAGGGCAGTGACTTTGACCTCCATACTCTTTTAAATATAGTAGATAAGACAGATCTTGTGTTGCCAAAATTCTTTGGCCCACATTTAAGTGGGATGCAGCTAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(3), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-221-3p(1), mmu-miR-34a-5p(1), mmu-miR-31-5p(1), mmu-miR-152-3p(1), mmu-miR-320-3p(1), mmu-miR-326-3p(1), mmu-miR-181b-5p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-224-5p(1), mmu-miR-27a-5p(1), mmu-miR-1198-5p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-452-3p(1), mmu-miR-1904(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGAAACTAAAGGGCAGTGACTTTG
siRNA sets
CAAAGTCACTGCCCTTTAG, AAAGTCACTGCCCTTTAGT, TCACTGCCCTTTAGTTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGOLGA1_1584_ORF_1
1.126
0.204058095
0.999817543
1524
ORF sequence:
ATGTTTGCAAAACTAAAGAAGAAAATTGCAGAAGAGACTGCTGTTGCTCAGAGACCAGGCGGCACAACAAGGATCCCACGCTCTGTGAGCAAGGAGTCTGTGGCCTCCATGGGAGCTGACTCTGGAGATGATTTTGCTTCCGATGGTAGCAGCTCCAGAGAAGATCTCTCATCCCAGCTTTTGAGAAGAAATGAACAGATACGGAAGTTAGAGGCCAGACTTTCTGACTATGCTGAACAGGTCCGAAACTTGCAGAAGATAAAAGAGAAGCTTGAAATTGCATTAGAAAAACACCAGGATTCTTCCATGCGGAAATTTCAAGAACAGAATGAAACATTCCAAGCCAGCAGAGCCAAAATGGCAGAAGGACTGGCTTTGGCTTTAGCCAGGAAGGACCAGGAATGGTCAGAAAAGATGGAACAGCTTGAAAAGGATAAAAGGTTTCTAACATCTCAGCTGCAAGAAGTAAAGAACCAGAGCTTGAGCCTTTTTCAAAAAAGAGATGAAATTGATGAATTAGAGGGGTTCCAGCAGCAGGAAATCAGCAAAGTAAAACACATGCTTTTAAAAAAAGAAGAATGCTTGGGGAAGATGGAGCAAGAATTGGATGCACGAACCAGGGAACTTAACCGTACTCAGGAGGAGTTGGTGACTTCTAATCAGTTGTCATCTGACTTAAACGAGCGGCTAGAAGAATTGCAGAGACACTGCTCAACGCTGGAAGAGCAGAGAGATCACTTGACAGCTTCAAAAGCAGGTGCAGAACATAAGATTGTAGTCCTGGAACAAAAGGAACAAGAGCTCCAGGCGATCATCCAGCAGCATTCCATTGACCTACAAAAGGTCACTGCTGAAACGCAAGAAAAAGAAAAAGTCATCACACATCTACAAGAGAAGGTTATATTCTTGGAAAAGAGACTAGAACAGAATTTATCAGGAGAAGATCATGTACAAGAACTCTTGAAAGAGAAAACAGTTGCTGAACAGAATCTGGAAGATACTAGGCAGCAGCTCTTGGCTGCCAGGAACAGCCACACCAAGGCACTTTACCTCCTGGAGACTCGGGTGAAGGACCTAGAGCGGAGTCTACAGGCTGCTGAAGAACAGCTAAGCCAGAGTAGAAATGTTGTGGCTGACCAGGAAGCCCAGATCCAGAAGCTTATCACTACAAACCAGGAGAACAGCCTTTCCCAGCAGCAGGTTCTTGCCCTGGAACAGCATTGCAGGGAGCGCATCCATGCACTGGAGGCCCAAATTGAAGCTCTGGAACAAACACGGGTAGCTGACCAGATAGCCTCAGAGCAGGGAATGCTACAACTGCAGCAGGAAAATGTGGCCCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAAAGGGCAGTGACTTTGACCTCCATACTCTTTTAAATATAGTAGATAAGACAGATCTTGTGTTGCCAAAATTCTTTGGCCCACATTTAAGTGGGATGCAGCTAGCCTGGAGGTGTGAATGA
Peptide sequence:
MFAKLKKKIAEETAVAQRPGGTTRIPRSVSKESVASMGADSGDDFASDGSSSREDLSSQLLRRNEQIRKLEARLSDYAEQVRNLQKIKEKLEIALEKHQDSSMRKFQEQNETFQASRAKMAEGLALALARKDQEWSEKMEQLEKDKRFLTSQLQEVKNQSLSLFQKRDEIDELEGFQQQEISKVKHMLLKKEECLGKMEQELDARTRELNRTQEELVTSNQLSSDLNERLEELQRHCSTLEEQRDHLTASKAGAEHKIVVLEQKEQELQAIIQQHSIDLQKVTAETQEKEKVITHLQEKVIFLEKRLEQNLSGEDHVQELLKEKTVAEQNLEDTRQQLLAARNSHTKALYLLETRVKDLERSLQAAEEQLSQSRNVVADQEAQIQKLITTNQENSLSQQQVLALEQHCRERIHALEAQIEALEQTRVADQIASEQGMLQLQQENVALKESRNECEHSLQHHQLELKKLKGSDFDLHTLLNIVDKTDLVLPKFFGPHLSGMQLAWRCE
IRESfinder result
IRESfinder score
0.581390514
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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