top of page
mmu_circGnas_318
Basic information of circular RNA
PanCircBase ID:
mmu_circGnas_318
mm39_Chr. Position ID:
chr2|174178539|174185090|+
Gene name:
Gnas
Transcript:
ENSMUST00000109096.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,4906,5063,6453
Genomic length:
6551
Exon/intron sizes in nt:
45,55,120,98
Flanking exon/introns:
chr2:174176077-174178539|chr2:174185090-174186867
Splice length:
318
Other database IDs:
circBase: hsa_circ_0060996, CIRCpedia: HSA_CIRCpedia_96184
Human Ortholog IDs:
Mature spliced Sequence:
GGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACTAAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCTGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGTGCCGAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTGCGTGCCTGCTACGAGCGCTCCAATGAGTACCAGCTGATTGACTGTGCCCAGTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGTGGGAGGATGAGGGAG
60.03
GTCCTGCACTTTAGTGGCCT
59.96
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTGTGGGAGGATGAGGGA
60.03
GTCCTGCACTTTAGTGGCCT
59.96
146
PCR template sequence:
CTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTGCGTGCCTGCTACGAGCGCTCCAATGAGTACCAGCTGATTGACTGTGCCCAGTAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACTAAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTGTGCCCAGTAGGGCGGCGAAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGNAS_318_ORF_1
1.121
0.589726731
0.670385546
204
ORF sequence:
ATGAGCAACCTGGTGCCCCCTGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGTGCCGAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTGCGTGCCTGCTACGAGCGCTCCAATGAGTACCAGCTGATTGACTGTGCCCAGTAG
Peptide sequence:
MSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQ
IRESfinder result
IRESfinder score
0.296934837
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page