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mmu_circGm609_300
Basic information of circular RNA
PanCircBase ID:
mmu_circGm609_300
mm39_Chr. Position ID:
chr16|45262809|45263109|-
Gene name:
Gm609
Transcript:
ENSMUST00000144346.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
300
Exon/intron sizes in nt:
300
Flanking exon/introns:
chr16:45240306-45262809|chr16:45263109-45264189
Splice length:
300
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTGTATCTGAACTAGTAACTGAGCTCCGGTCTGCTCCTGGCTCTGAAGATCTTCATAGCCTTCTTTGTTCAGCTGTGGGAAAGCCTGCCCCTGGAATCTCCGTTTACCCTTCACAAGTCACAGTGCAGGCACCACAAGAGTACTTTGCTCAGAACGCCAATGCCACGGTGACTGTCACTAAAATCTACAACATCTCTTTGAAAACTGCGAGGTCCCTGGGTCTCCAGGACTTGGTTGTGTCAATGACTCACTCTGTAAGAAACGAAGAGAAAATAGTCCGATTGCCAGTCAATCAAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAAACTGCGAGGTCCCTGG
59.93
CCACAGCTGAACAAAGAAGGC
60
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAACTGCGAGGTCCCTGG
59.93
AGATCTTCAGAGCCAGGAGC
58.88
152
PCR template sequence:
AAAACTGCGAGGTCCCTGGGTCTCCAGGACTTGGTTGTGTCAATGACTCACTCTGTAAGAAACGAAGAGAAAATAGTCCGATTGCCAGTCAATCAAGGAGCTGTATCTGAACTAGTAACTGAGCTCCGGTCTGCTCCTGGCTCTGAAGATCTTCATAGCCTTCTTTGTTCAGCTGTGGGAAAGCCTGCCCCTGGAATCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
GTCAATCAAGGAGCTGTATCTGAACT
siRNA sets
TTCAGATACAGCTCCTTGA, GATACAGCTCCTTGATTGA, GTTCAGATACAGCTCCTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGM609_300_ORF_1
0.9178
0.105148614
0.367203876
357
ORF sequence:
ATGACTCACTCTGTAAGAAACGAAGAGAAAATAGTCCGATTGCCAGTCAATCAAGGAGCTGTATCTGAACTAGTAACTGAGCTCCGGTCTGCTCCTGGCTCTGAAGATCTTCATAGCCTTCTTTGTTCAGCTGTGGGAAAGCCTGCCCCTGGAATCTCCGTTTACCCTTCACAAGTCACAGTGCAGGCACCACAAGAGTACTTTGCTCAGAACGCCAATGCCACGGTGACTGTCACTAAAATCTACAACATCTCTTTGAAAACTGCGAGGTCCCTGGGTCTCCAGGACTTGGTTGTGTCAATGACTCACTCTGTAAGAAACGAAGAGAAAATAGTCCGATTGCCAGTCAATCAAGGA
Peptide sequence:
MTHSVRNEEKIVRLPVNQGAVSELVTELRSAPGSEDLHSLLCSAVGKPAPGISVYPSQVTVQAPQEYFAQNANATVTVTKIYNISLKTARSLGLQDLVVSMTHSVRNEEKIVRLPVNQG
IRESfinder result
IRESfinder score
0.662002503
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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