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mmu_circGm45062_114

Basic information of circular RNA
PanCircBase ID:

mmu_circGm45062_114

mm39_Chr. Position ID:

chr6|8573184|8573298|+

Gene name:

Gm45062

Transcript:

ENSMUST00000162564.7

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

1

Exon/intron indexes:

7

Exon/intron offsets:

0

Genomic length:

114

Exon/intron sizes in nt:

114

Flanking exon/introns:

chr6:8558586-8573184|chr6:8573298-8579586

Splice length:

114

Other database IDs:

circBase: hsa_circ_0134786, CIRCpedia: HSA_CIRCpedia_144973

Human Ortholog IDs:
Mature spliced Sequence:

ATTGCCAAACTTAGGCAACAGCTACAACGCAGCAAGCAAAGTAGTCGGCATAGTAAAGAGAAGGACAGACAGTCACCTCTCCATGGCAACCACATAACAATCAGTCATACTCAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CCTCTCCATGGCAACCACAT

60.03

TGCGTTGTAGCTGTTGCCTA

59.97

70

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GACAGTCACCTCTCCATGGC

60.11

TGCGTTGTAGCTGTTGCCTA

59.97

78

PCR template sequence:

GAGAAGGACAGACAGTCACCTCTCCATGGCAACCACATAACAATCAGTCATACTCAGATTGCCAAACTTAGGCAACAGCTACAACGCAGCAAGCAAAGTAGTCGGCATAGTAAA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

1

Interacting miRNAs (#binding sites)

mmu-miR-134-5p(1)

siRNAs for circular RNA silencing
Junction sequence

CAGTCATACTCAGATTGCCAAACTTA

siRNA sets

TAAGTTTGGCAATCTGAGT, AAGTTTGGCAATCTGAGTA, TTTGGCAATCTGAGTATGA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.175564448

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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Institute of Life sciences, Bhubaneswar, India

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