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mmu_circGlyr1_765
Basic information of circular RNA
PanCircBase ID:
mmu_circGlyr1_765
mm39_Chr. Position ID:
chr16|4847197|4866650|-
Gene name:
Glyr1
Transcript:
ENSMUST00000115844.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
9,8,7,6,5,4,3,2
Exon/intron offsets:
0,169,851,2553,6902,7585,18400,19416
Genomic length:
19453
Exon/intron sizes in nt:
74,51,57,87,240,139,80,37
Flanking exon/introns:
chr16:4844471-4847197|chr16:4866650-4867669
Splice length:
765
Other database IDs:
circAtlas: mmu-Glyr1_0027
Human Ortholog IDs:
Mature spliced Sequence:
GGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTTTTTGGAACAGAAGATCATGCCTGGATCAAAGTGGAACAGCTAAAGCCTTACCATGCTCACAAGGAGGAGATGATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCCAAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGAGAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACATGGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAAGAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGCCAGTCAAAGATGCAGATCCTCATTTTCATCATTTTCTGTTGAGCCAAACAGAGAAGCCAGCTGTCTGTTACCAGGCAATCACAAAGAAGTTGAAAATATGTGAAGAGGAAACTGGTTCCACCTCCATCCAGGCAGCAGACAGCACAGCTGTGAATGGCAGCATTACACCCACAGACAAAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGTTCCACCTCCATCCAG
59.75
AGCATTTCTTTCCACGTGGT
58.02
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGGTTCCACCTCCATCCA
60.48
AGCATTTCTTTCCACGTGGT
58.02
157
PCR template sequence:
CAAAGAAGTTGAAAATATGTGAAGAGGAAACTGGTTCCACCTCCATCCAGGCAGCAGACAGCACAGCTGTGAATGGCAGCATTACACCCACAGACAAAAAGGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-149-5p(1), mmu-miR-351-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CCACAGACAAAAAGGGGAAACTGGGC
siRNA sets
TTTCCCCTTTTTGTCTGTG,TTCCCCTTTTTGTCTGTGG,AGTTTCCCCTTTTTGTCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGLYR1_765_ORF_1
1.0528
0.256679791
0.999466439
1359
ORF sequence:
ATGATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCCAAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGAGAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACATGGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAAGAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGCCAGTCAAAGATGCAGATCCTCATTTTCATCATTTTCTGTTGAGCCAAACAGAGAAGCCAGCTGTCTGTTACCAGGCAATCACAAAGAAGTTGAAAATATGTGAAGAGGAAACTGGTTCCACCTCCATCCAGGCAGCAGACAGCACAGCTGTGAATGGCAGCATTACACCCACAGACAAAAAGGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTTTTTGGAACAGAAGATCATGCCTGGATCAAAGTGGAACAGCTAAAGCCTTACCATGCTCACAAGGAGGAGATGATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCCAAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGAGAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACATGGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAAGAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGCCAGTCAAAGATGCAGATCCTCATTTTCATCATTTTCTGTTGAGCCAAACAGAGAAGCCAGCTGTCTGTTACCAGGCAATCACAAAGAAGTTGAAAATATGTGAAGAGGAAACTGGTTCCACCTCCATCCAGGCAGCAGACAGCACAGCTGTGAATGGCAGCATTACACCCACAGACAAA
Peptide sequence:
MIKINKGKRFQQAVDAVEEFLRRAKGKDQTSSHTSADDKNRRNSSEERSRPNSGDEKRKLSLSEGKVKKNMGEGKKRVTSGSADRGSKCLKRAQEQSPRKRGRPPKDEKDLTIPESSTVKGMMAGPMAAFKWQPTATEPVKDADPHFHHFLLSQTEKPAVCYQAITKKLKICEEETGSTSIQAADSTAVNGSITPTDKKGKLGRYPPWPGKIVNPPKDLKKPRGKKCFFVKFFGTEDHAWIKVEQLKPYHAHKEEMIKINKGKRFQQAVDAVEEFLRRAKGKDQTSSHTSADDKNRRNSSEERSRPNSGDEKRKLSLSEGKVKKNMGEGKKRVTSGSADRGSKCLKRAQEQSPRKRGRPPKDEKDLTIPESSTVKGMMAGPMAAFKWQPTATEPVKDADPHFHHFLLSQTEKPAVCYQAITKKLKICEEETGSTSIQAADSTAVNGSITPTDK
IRESfinder result
IRESfinder score
0.295867747
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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