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mmu_circGlyr1_583
Basic information of circular RNA
PanCircBase ID:
mmu_circGlyr1_583
mm39_Chr. Position ID:
chr16|4849750|4866650|-
Gene name:
Glyr1
Transcript:
ENSMUST00000115844.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,5,4,3,2
Exon/intron offsets:
0,4349,5032,15847,16863
Genomic length:
16900
Exon/intron sizes in nt:
87,240,139,80,37
Flanking exon/introns:
chr16:4848105-4849750|chr16:4866650-4867669
Splice length:
583
Other database IDs:
circAtlas: mmu-Glyr1_0015
circBase: hsa_circ_0037763
Human Ortholog IDs:
Mature spliced Sequence:
GGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTTTTTGGAACAGAAGATCATGCCTGGATCAAAGTGGAACAGCTAAAGCCTTACCATGCTCACAAGGAGGAGATGATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCCAAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGAGAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACATGGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAAGAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGACCTCACCATCCCTGA
59.88
AGCATTTCTTTCCACGTGGT
58.02
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGGGGATGATGGCTGGAC
59.74
AGCATTTCTTTCCACGTGGT
58.02
144
PCR template sequence:
AAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGGGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-351-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AACAGCGACCGAGGGGGAAACTGGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGLYR1_583_ORF_1
0.9651
0.258867749
0.709932821
459
ORF sequence:
ATGATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCCAAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGAGAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACATGGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAAGAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCACTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGGGGGAAACTGGGCCGGTATCCTCCCTGGCCAGGAAAGATTGTTAA
Peptide sequence:
MIKINKGKRFQQAVDAVEEFLRRAKGKDQTSSHTSADDKNRRNSSEERSRPNSGDEKRKLSLSEGKVKKNMGEGKKRVTSGSADRGSKCLKRAQEQSPRKRGRPPKDEKDLTIPESSTVKGMMAGPMAAFKWQPTATEGETGPVSSLARKDC
IRESfinder result
IRESfinder score
0.222296288
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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