top of page
mmu_circGlod4_570
Basic information of circular RNA
PanCircBase ID:
mmu_circGlod4_570
mm39_Chr. Position ID:
chr11|76123847|76128639|-
Gene name:
Glod4
Transcript:
ENSMUST00000169532.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
8,7,6,5,4
Exon/intron offsets:
0,180,878,1367,4647
Genomic length:
4792
Exon/intron sizes in nt:
87,11,48,71,37,145
Flanking exon/introns:
chr11:76012422-76123847|chr11:76128639-76130277
Splice length:
570
Other database IDs:
circAtlas: mmu-Glod4_0009
CIRCpedia: HSA_CIRCpedia_67113
Human Ortholog IDs:
Mature spliced Sequence:
GGAATCACGCTTGCTTCTAGCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAGATCCTGTATTAAAGGTGACTCTAGCAGTGTCTGATCTCCAGAAGTCCTTGAACTACTGGTCTAATCTCCTGGGAATGAAAATTTATGAGCAAGATGAGGAAAAGCAACGAGCTTTGCTGGGCTACGCTGATAACCAGTGTAAGCTAGAGCTACAGGGCATCCAGGGTGCAGTTGATCATGCAGCGGCCTTTGGAAGAATTGCCTTTTCTTGTCCTCAGAAAGAGTTGCCAGACTTAGAAGACTTGATGAAAAGGGAGAGCCACTCGATCCTGACTCCGTTGGTGAGTCTGGACACCCCAGGGAAAGCCACAGTGCAAGTGGTCATCCTGGCTGACCCTGATGGACATGAAATTTGCTTTGTTGGGGATGAAGCTTTTCGAGAACTCTCTAAGATGGATCCAAAAGGAAGCAAATTATTGGATGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTGACCCTGATGGACATG
60.47
AGCTTCCTGGCATTGCTGAC
60.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGACCCTGATGGACATGA
59.46
AGCTTCCTGGCATTGCTGAC
60.96
143
PCR template sequence:
CCTGGCTGACCCTGATGGACATGAAATTTGCTTTGTTGGGGATGAAGCTTTTCGAGAACTCTCTAAGATGGATCCAAAAGGAAGCAAATTATTGGATGATGGAATCACGCTTGCTTCTAGCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-383-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTATTGGATGATGGAATCACGCTTG
siRNA sets
AAGCGTGATTCCATCATCC, CAAGCGTGATTCCATCATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGLOD4_570_ORF_1
1.0518
0.230945934
0.986301697
921
ORF sequence:
ATGAAAATTTATGAGCAAGATGAGGAAAAGCAACGAGCTTTGCTGGGCTACGCTGATAACCAGTGTAAGCTAGAGCTACAGGGCATCCAGGGTGCAGTTGATCATGCAGCGGCCTTTGGAAGAATTGCCTTTTCTTGTCCTCAGAAAGAGTTGCCAGACTTAGAAGACTTGATGAAAAGGGAGAGCCACTCGATCCTGACTCCGTTGGTGAGTCTGGACACCCCAGGGAAAGCCACAGTGCAAGTGGTCATCCTGGCTGACCCTGATGGACATGAAATTTGCTTTGTTGGGGATGAAGCTTTTCGAGAACTCTCTAAGATGGATCCAAAAGGAAGCAAATTATTGGATGATGGAATCACGCTTGCTTCTAGCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAGATCCTGTATTAAAGGTGACTCTAGCAGTGTCTGATCTCCAGAAGTCCTTGAACTACTGGTCTAATCTCCTGGGAATGAAAATTTATGAGCAAGATGAGGAAAAGCAACGAGCTTTGCTGGGCTACGCTGATAACCAGTGTAAGCTAGAGCTACAGGGCATCCAGGGTGCAGTTGATCATGCAGCGGCCTTTGGAAGAATTGCCTTTTCTTGTCCTCAGAAAGAGTTGCCAGACTTAGAAGACTTGATGAAAAGGGAGAGCCACTCGATCCTGACTCCGTTGGTGAGTCTGGACACCCCAGGGAAAGCCACAGTGCAAGTGGTCATCCTGGCTGACCCTGATGGACATGAAATTTGCTTTGTTGGGGATGAAGCTTTTCGAGAACTCTCTAAGATGGATCCAAAAGGAAGCAAATTATTGGATGAT
Peptide sequence:
MKIYEQDEEKQRALLGYADNQCKLELQGIQGAVDHAAAFGRIAFSCPQKELPDLEDLMKRESHSILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPKGSKLLDDGITLASSQAVSNARKLEWPLSKVAEGIFETEAPGGYKFYLQDRSPSQSDPVLKVTLAVSDLQKSLNYWSNLLGMKIYEQDEEKQRALLGYADNQCKLELQGIQGAVDHAAAFGRIAFSCPQKELPDLEDLMKRESHSILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPKGSKLLDD
IRESfinder result
IRESfinder score
0.470324439
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page