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mmu_circGlod4_316
Basic information of circular RNA
PanCircBase ID:
mmu_circGlod4_316
mm39_Chr. Position ID:
chr11|76128494|76131748|-
Gene name:
Glod4
Transcript:
ENSMUST00000169532.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,1783,3204
Genomic length:
3254
Exon/intron sizes in nt:
145,121,50
Flanking exon/introns:
chr11:76125351-76128494|chr11:76131748-76134380
Splice length:
316
Other database IDs:
CIRCpedia: MMU_CIRCpedia_36469, circAtlas: mmu-Glod4_0003
circAtlas: hsa-GLOD4_0003, CIRCpedia: HSA_CIRCpedia_77269
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTCCGCCATGAGGAATTTGAAGAAGGCTGCAAAGCTGCATGCAATGGGCCTTATGATGGGAAGTGGAGTAAAACAATGGTGGGATTTGGGCCCGAGGATGATCATTTTGTTGCAGAATTGACATACAATTATGGCATCGGAGACTACAAGCTTGGCAATGACTTCATGGGAATCACGCTTGCTTCTAGCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAACTGAAGCCCCAGGAGG
60.32
GGCCCAAATCCCACCATTGT
60.91
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGAAGCCCCAGGAGGATA
59.66
GGCCCAAATCCCACCATTGT
60.91
153
PCR template sequence:
CTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAGGTTCTCCGCCATGAGGAATTTGAAGAAGGCTGCAAAGCTGCATGCAATGGGCCTTATGATGGGAAGTGGAGTAAAACAATGGTGGGATTTGGGCCCGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCATCTCAGTCAGGTTCTCCGCCATG
siRNA sets
GAGAACCTGACTGAGATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGLOD4_316_ORF_1
0.8107
0.168228362
0.223745922
252
ORF sequence:
ATGGTGGGATTTGGGCCCGAGGATGATCATTTTGTTGCAGAATTGACATACAATTATGGCATCGGAGACTACAAGCTTGGCAATGACTTCATGGGAATCACGCTTGCTTCTAGCCAAGCTGTCAGCAATGCCAGGAAGCTGGAGTGGCCACTGAGTAAAGTTGCAGAAGGGATTTTTGAAACTGAAGCCCCAGGAGGATATAAGTTCTACTTACAGGATAGAAGCCCATCTCAGTCAGGTTCTCCGCCATGA
Peptide sequence:
MVGFGPEDDHFVAELTYNYGIGDYKLGNDFMGITLASSQAVSNARKLEWPLSKVAEGIFETEAPGGYKFYLQDRSPSQSGSPP
IRESfinder result
IRESfinder score
0.317246771
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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