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mmu_circGet1_349
Basic information of circular RNA
PanCircBase ID:
mmu_circGet1_349
mm39_Chr. Position ID:
chr16|95953012|95955395|+
Gene name:
Get1
Transcript:
ENSMUST00000023913.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,1158,2268
Genomic length:
2383
Exon/intron sizes in nt:
1,66,68,115
Flanking exon/introns:
chr16:95946778-95953012|chr16:95955395-95957078
Splice length:
349
Other database IDs:
circBase: mmu_circ_0006510, CIRCpedia: MMU_CIRCpedia_35309, circAtlas: mmu-Wrb_0001
circBase: hsa_circ_0061766, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_35562
Human Ortholog IDs:
Mature spliced Sequence:
ATTTCCAGGGTGCTGCAGAAGGATGCAGAGCAGGAGTCACAGATGAGGGCAGAGATCCAGGGCATGAAGCAGGAGCTGTCCACCGTCAACATGATGGACGAGTTTGCCAGATACGCCAGGCTGGAAAGGAAGATCAACAAGATGACGGACAAGCTCAAAACGCACGTGAAGGCACGGACAGCTCAGTTGGCCAAGATCAAGTGGTTTATAAGTGTTGCTTTCTACATACTACAAGCAGCGCTGATGATCTCGCTCATTTGGAAGTATTACTCCGTCCCTGTGGCTGTGGTGCCCAGCAAGTGGATCACCCCACTAGACCGCCTGGTAGCCTTTCCTACTCGAGTAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTACTCCGTCCCTGTGGCT
60.62
TTCATGCCCTGGATCTCTGC
59.82
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTACTCCGTCCCTGTGGCT
60.62
TGCTTCATGCCCTGGATCTC
59.82
155
PCR template sequence:
TCGCTCATTTGGAAGTATTACTCCGTCCCTGTGGCTGTGGTGCCCAGCAAGTGGATCACCCCACTAGACCGCCTGGTAGCCTTTCCTACTCGAGTAGCAGATTTCCAGGGTGCTGCAGAAGGATGCAGAGCAGGAGTCACAGATGAGGGCAGAGATCCAGGGCATGAAGCAGGAGCTGTCCACCGTCAACATGATGGACG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-184-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCGAGTAGCAGATTTCCAGGGTGC
siRNA sets
CTGGAAATCTGCTACTCGA, TGGAAATCTGCTACTCGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGET1_349_ORF_1
0.9936
0.261852971
0.915164091
654
ORF sequence:
ATGAGGGCAGAGATCCAGGGCATGAAGCAGGAGCTGTCCACCGTCAACATGATGGACGAGTTTGCCAGATACGCCAGGCTGGAAAGGAAGATCAACAAGATGACGGACAAGCTCAAAACGCACGTGAAGGCACGGACAGCTCAGTTGGCCAAGATCAAGTGGTTTATAAGTGTTGCTTTCTACATACTACAAGCAGCGCTGATGATCTCGCTCATTTGGAAGTATTACTCCGTCCCTGTGGCTGTGGTGCCCAGCAAGTGGATCACCCCACTAGACCGCCTGGTAGCCTTTCCTACTCGAGTAGCAGATTTCCAGGGTGCTGCAGAAGGATGCAGAGCAGGAGTCACAGATGAGGGCAGAGATCCAGGGCATGAAGCAGGAGCTGTCCACCGTCAACATGATGGACGAGTTTGCCAGATACGCCAGGCTGGAAAGGAAGATCAACAAGATGACGGACAAGCTCAAAACGCACGTGAAGGCACGGACAGCTCAGTTGGCCAAGATCAAGTGGTTTATAAGTGTTGCTTTCTACATACTACAAGCAGCGCTGATGATCTCGCTCATTTGGAAGTATTACTCCGTCCCTGTGGCTGTGGTGCCCAGCAAGTGGATCACCCCACTAGACCGCCTGGTAGCCTTTCCTACTCGAGTAGC
Peptide sequence:
MRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVADFQGAAEGCRAGVTDEGRDPGHEAGAVHRQHDGRVCQIRQAGKEDQQDDGQAQNAREGTDSSVGQDQVVYKCCFLHTTSSADDLAHLEVLLRPCGCGAQQVDHPTRPPGSLSYSSS
IRESfinder result
IRESfinder score
0.220316106
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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