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mmu_circGbe1_1185
Basic information of circular RNA
PanCircBase ID:
mmu_circGbe1_1185
mm39_Chr. Position ID:
chr16|70230436|70292287|+
Gene name:
Gbe1
Transcript:
ENSMUST00000163832.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
4,5,6,7,8,9,10,11,12
Exon/intron offsets:
0,965,3379,7561,44757,50272,54479,58385,61679
Genomic length:
61851
Exon/intron sizes in nt:
122,136,91,210,116,128,99,111,172
Flanking exon/introns:
chr16:70198741-70230436|chr16:70292287-70324004
Splice length:
1185
Other database IDs:
circAtlas: mmu-Gbe1_0026
Human Ortholog IDs:
Mature spliced Sequence:
TTATTACTAGTAAGAGTGGCGAGATACTGTATCGCATTTCCCCATGGGCAAAGTATGTGGTTCGTGAGAACAACAACGTGAACTATGATTGGATACACTGGGCTCCAGAAGACCCCTATAAATTCAAGCATTCCAGACCTAAGAAACCAAGAAGCCTAAGAATTTATGAATCTCATGTGGGAATTTCTTCCCACGAAGGAAAAATAGCTTCTTACAAACATTTTACCAGCAATGTACTACCAAGAATCAAAGACCTTGGGTATAACTGCATTCAGTTGATGGCGATCATGGAACATGCTTACTATGCCAGTTTTGGGTACCAAATCACGAGCTTTTTTGCAGCTTCAAGTCGTTATGGAACTCCTGAAGAGCTGAAAGAACTTGTTGACACAGCACATTCGATGGGCATTGTAGTCCTCCTAGATGTGGTTCATAGCCATGCTTCAAAAAATTCAGAAGATGGGTTAAACATGTTTGATGGGACCGATTCCTGTTATTTTCACTCTGGACCCCGAGGGACCCATGATCTTTGGGACAGTAGATTATTTATCTATTCCAGCTGGGAAGTTTTAAGATTCCTTCTGTCGAACATAAGATGGTGGTTGGAGGAGTACTGCTTTGATGGCTTCCGTTTTGATGGTGTCACCTCTATGCTCTATCATCACCACGGAATGGGTCAAGGTTTTTCGGGTGACTATAATGAATATTTTGGACTACAAGTAGATGAAGATGCTTTGATTTATCTCATGTTGGCAAATCATTTGGCTCACACGTTGTACCCAGACTCGATAACAATAGCAGAGGATGTATCAGGGATGCCGGCTCTCTGTTCTCCAACTTCCCAGGGAGGCGGTGGTTTTGACTACAGATTAGCAATGGCTATTCCAGATAAATGGATCCAATTACTTAAGGAATTTAAAGATGAAGACTGGAATATGGGCAATATAGTGTATACTCTCACCAATCGACGCTACCTTGAAAAATGTGTGGCTTATGCAGAGAGTCATGATCAGGCATTGGTTGGTGACAAGACACTGGCGTTTTGGTTGATGGACGCTGAGATGTACACGAACATGAGCGTTCTGGCCCCTTTCACTCCAGTAATTGATCGAGGAATACAGCTTCATAAGATGATTCGTCTCATCACTCACGGGCTCGGTGGAGAAGGCTATCTCAATTTCATGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCCTTTCACTCCAGTAATTG
59.24
CCACATACTTTGCCCATGGG
58.89
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCCTTTCACTCCAGTAAT
57.19
CCACATACTTTGCCCATGGG
58.89
160
PCR template sequence:
GCCCCTTTCACTCCAGTAATTGATCGAGGAATACAGCTTCATAAGATGATTCGTCTCATCACTCACGGGCTCGGTGGAGAAGGCTATCTCAATTTCATGGTTATTACTAGTAAGAGTGGCGAGATACTGTATCGCATTTCCCCATGGGCAAAGTATGTGGTTCGTGAGAACAACAACGTGAACTATGATTGGATACACTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-191-5p(1), mmu-miR-127-5p(1), mmu-miR-188-3p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCAATTTCATGGTTATTACTAGTAA
siRNA sets
TTACTAGTAATAACCATGA, GTAATAACCATGAAATTGA, TACTAGTAATAACCATGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGBE1_1185_ORF_1
0.8518
0.070745632
0.999991403
2091
ORF sequence:
ATGGCGATCATGGAACATGCTTACTATGCCAGTTTTGGGTACCAAATCACGAGCTTTTTTGCAGCTTCAAGTCGTTATGGAACTCCTGAAGAGCTGAAAGAACTTGTTGACACAGCACATTCGATGGGCATTGTAGTCCTCCTAGATGTGGTTCATAGCCATGCTTCAAAAAATTCAGAAGATGGGTTAAACATGTTTGATGGGACCGATTCCTGTTATTTTCACTCTGGACCCCGAGGGACCCATGATCTTTGGGACAGTAGATTATTTATCTATTCCAGCTGGGAAGTTTTAAGATTCCTTCTGTCGAACATAAGATGGTGGTTGGAGGAGTACTGCTTTGATGGCTTCCGTTTTGATGGTGTCACCTCTATGCTCTATCATCACCACGGAATGGGTCAAGGTTTTTCGGGTGACTATAATGAATATTTTGGACTACAAGTAGATGAAGATGCTTTGATTTATCTCATGTTGGCAAATCATTTGGCTCACACGTTGTACCCAGACTCGATAACAATAGCAGAGGATGTATCAGGGATGCCGGCTCTCTGTTCTCCAACTTCCCAGGGAGGCGGTGGTTTTGACTACAGATTAGCAATGGCTATTCCAGATAAATGGATCCAATTACTTAAGGAATTTAAAGATGAAGACTGGAATATGGGCAATATAGTGTATACTCTCACCAATCGACGCTACCTTGAAAAATGTGTGGCTTATGCAGAGAGTCATGATCAGGCATTGGTTGGTGACAAGACACTGGCGTTTTGGTTGATGGACGCTGAGATGTACACGAACATGAGCGTTCTGGCCCCTTTCACTCCAGTAATTGATCGAGGAATACAGCTTCATAAGATGATTCGTCTCATCACTCACGGGCTCGGTGGAGAAGGCTATCTCAATTTCATGGTTATTACTAGTAAGAGTGGCGAGATACTGTATCGCATTTCCCCATGGGCAAAGTATGTGGTTCGTGAGAACAACAACGTGAACTATGATTGGATACACTGGGCTCCAGAAGACCCCTATAAATTCAAGCATTCCAGACCTAAGAAACCAAGAAGCCTAAGAATTTATGAATCTCATGTGGGAATTTCTTCCCACGAAGGAAAAATAGCTTCTTACAAACATTTTACCAGCAATGTACTACCAAGAATCAAAGACCTTGGGTATAACTGCATTCAGTTGATGGCGATCATGGAACATGCTTACTATGCCAGTTTTGGGTACCAAATCACGAGCTTTTTTGCAGCTTCAAGTCGTTATGGAACTCCTGAAGAGCTGAAAGAACTTGTTGACACAGCACATTCGATGGGCATTGTAGTCCTCCTAGATGTGGTTCATAGCCATGCTTCAAAAAATTCAGAAGATGGGTTAAACATGTTTGATGGGACCGATTCCTGTTATTTTCACTCTGGACCCCGAGGGACCCATGATCTTTGGGACAGTAGATTATTTATCTATTCCAGCTGGGAAGTTTTAAGATTCCTTCTGTCGAACATAAGATGGTGGTTGGAGGAGTACTGCTTTGATGGCTTCCGTTTTGATGGTGTCACCTCTATGCTCTATCATCACCACGGAATGGGTCAAGGTTTTTCGGGTGACTATAATGAATATTTTGGACTACAAGTAGATGAAGATGCTTTGATTTATCTCATGTTGGCAAATCATTTGGCTCACACGTTGTACCCAGACTCGATAACAATAGCAGAGGATGTATCAGGGATGCCGGCTCTCTGTTCTCCAACTTCCCAGGGAGGCGGTGGTTTTGACTACAGATTAGCAATGGCTATTCCAGATAAATGGATCCAATTACTTAAGGAATTTAAAGATGAAGACTGGAATATGGGCAATATAGTGTATACTCTCACCAATCGACGCTACCTTGAAAAATGTGTGGCTTATGCAGAGAGTCATGATCAGGCATTGGTTGGTGACAAGACACTGGCGTTTTGGTTGATGGACGCTGAGATGTACACGAACATGAGCGTTCTGGCCCCTTTCACTCCAGTAATTGATCGAGGAATACAGCTTCATAAGATGATTCGTCTCATCACTCACGGGCTCGGTGGAGAAGGCTATCTCAATTTCATG
Peptide sequence:
MAIMEHAYYASFGYQITSFFAASSRYGTPEELKELVDTAHSMGIVVLLDVVHSHASKNSEDGLNMFDGTDSCYFHSGPRGTHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYCFDGFRFDGVTSMLYHHHGMGQGFSGDYNEYFGLQVDEDALIYLMLANHLAHTLYPDSITIAEDVSGMPALCSPTSQGGGGFDYRLAMAIPDKWIQLLKEFKDEDWNMGNIVYTLTNRRYLEKCVAYAESHDQALVGDKTLAFWLMDAEMYTNMSVLAPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMVITSKSGEILYRISPWAKYVVRENNNVNYDWIHWAPEDPYKFKHSRPKKPRSLRIYESHVGISSHEGKIASYKHFTSNVLPRIKDLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGTPEELKELVDTAHSMGIVVLLDVVHSHASKNSEDGLNMFDGTDSCYFHSGPRGTHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYCFDGFRFDGVTSMLYHHHGMGQGFSGDYNEYFGLQVDEDALIYLMLANHLAHTLYPDSITIAEDVSGMPALCSPTSQGGGGFDYRLAMAIPDKWIQLLKEFKDEDWNMGNIVYTLTNRRYLEKCVAYAESHDQALVGDKTLAFWLMDAEMYTNMSVLAPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFM
IRESfinder result
IRESfinder score
0.666034425
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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