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mmu_circGas5_181
Basic information of circular RNA
PanCircBase ID:
mmu_circGas5_181
mm39_Chr. Position ID:
chr1|160863552|160865842|+
Gene name:
Gas5
Transcript:
ENSMUST00000161005.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,225,566,837,2242
Genomic length:
2290
Exon/intron sizes in nt:
32,37,31,33,48
Flanking exon/introns:
chr1:160863374-160863552|chr1:160865842-160866026
Splice length:
181
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTCTGTGGCAAAGGAGGATGAAGGCTTACGAGGACTCGTCAGGAAGCTGGATAACAGAGCGAGCGCAATGTGCTAGAATAGAAGACCAGAAAATGAAATGGTGGAGTTTGAGGCTGGATAGACAGTTTGAAAGGGCTCCTGTGACAAGTGGACATGCAGTGACTGCACCTTTGTTTCTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGGTGGAGTTTGAGGCTGG
59.96
GCTCGCTCTGTTATCCAGCT
59.9
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGGTGGAGTTTGAGGCTGG
59.96
CAGCTTCCTGACGAGTCCTC
59.83
133
PCR template sequence:
AATGAAATGGTGGAGTTTGAGGCTGGATAGACAGTTTGAAAGGGCTCCTGTGACAAGTGGACATGCAGTGACTGCACCTTTGTTTCTGAGTTCTGTGGCAAAGGAGGATGAAGGCTTACGAGGACTCGTCAGGAAGCTGGATAACAGAGCGAGCGCAATGTGCTAGAATAGAAGACCAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-18a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTGTTTCTGAGTTCTGTGGCAAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGAS5_181_ORF_1
0.5281
0.044698907
0.086679593
240
ORF sequence:
ATGAAGGCTTACGAGGACTCGTCAGGAAGCTGGATAACAGAGCGAGCGCAATGTGCTAGAATAGAAGACCAGAAAATGAAATGGTGGAGTTTGAGGCTGGATAGACAGTTTGAAAGGGCTCCTGTGACAAGTGGACATGCAGTGACTGCACCTTTGTTTCTGAGTTCTGTGGCAAAGGAGGATGAAGGCTTACGAGGACTCGTCAGGAAGCTGGATAACAGAGCGAGCGCAATGTGCTAG
Peptide sequence:
MKAYEDSSGSWITERAQCARIEDQKMKWWSLRLDRQFERAPVTSGHAVTAPLFLSSVAKEDEGLRGLVRKLDNRASAMC
IRESfinder result
IRESfinder score
0.392522098
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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