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mmu_circGalnt14_528
Basic information of circular RNA
PanCircBase ID:
mmu_circGalnt14_528
mm39_Chr. Position ID:
chr17|73832286|73852118|-
Gene name:
Galnt14
Transcript:
ENSMUST00000024858.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
8,7,6,5,4,3
Exon/intron offsets:
0,944,9947,10230,11554,19733
Genomic length:
19832
Exon/intron sizes in nt:
85,88,122,66,68,99
Flanking exon/introns:
chr17:73829716-73832286|chr17:73852118-73881798
Splice length:
528
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGTTCGCTGCTTGTGTATTGCACGGACCTTCCTCCCACCAGCATCATCATCACCTTCCACAACGAGGCACGTTCCACTCTACTCAGAACCATCCGCAGTGTGTTAAACCGAACCCCTATGCATCTGATCCAGGAAATCATATTAGTGGATGACTTTAGCAATGACCCCGAGGACTGCAAGCAACTCATCAAATTGCCCAAGGTGAAGTGCTTGCGCAACAATGAGCGGCAAGGTCTGGTCCGGTCTCGGATGCGAGGTGCTGACATTGCCCAAGGCACCACTCTGACCTTCCTGGACAGCCATTGTGAGGTGAACAGGGATTGGCTGCAGCCCCTGTTACACAGGGTCAAAGAGGACTACACAAGGGTTGTGTGCCCTGTGATTGACATCATTAACCTGGATACCTTCAACTATATTGAGTCTGCCTCAGAGCTCAGAGGGGGCTTTGACTGGAGCCTGCACTTCCAGTGGGAGCAGCTCTCCCTGGAGCAGAAAGCTTTGCGCCTAGACCCTACTGAGCCCATCCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCAGAGGGGGCTTTGACTG
60.04
GCCTCGTTGTGGAAGGTGAT
60.32
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCAGAGGGGGCTTTGACTG
60.04
GTAGAGTGGAACGTGCCTCG
60.46
178
PCR template sequence:
CAGAGCTCAGAGGGGGCTTTGACTGGAGCCTGCACTTCCAGTGGGAGCAGCTCTCCCTGGAGCAGAAAGCTTTGCGCCTAGACCCTACTGAGCCCATCCGATGTTCGCTGCTTGTGTATTGCACGGACCTTCCTCCCACCAGCATCATCATCACCTTCCACAACGAGGCACGTTCCACTCTACTCAGAACCATCCGCAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-709(1), mmu-miR-10b-5p(1), mmu-miR-705(1), mmu-miR-484(1), mmu-miR-540-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CTGAGCCCATCCGATGTTCGCTGCTT
siRNA sets
AGCGAACATCGGATGGGCT, AAGCAGCGAACATCGGATG, GAACATCGGATGGGCTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGALNT14_528_ORF_1
1.212
0.396387409
0.995326843
936
ORF sequence:
ATGCATCTGATCCAGGAAATCATATTAGTGGATGACTTTAGCAATGACCCCGAGGACTGCAAGCAACTCATCAAATTGCCCAAGGTGAAGTGCTTGCGCAACAATGAGCGGCAAGGTCTGGTCCGGTCTCGGATGCGAGGTGCTGACATTGCCCAAGGCACCACTCTGACCTTCCTGGACAGCCATTGTGAGGTGAACAGGGATTGGCTGCAGCCCCTGTTACACAGGGTCAAAGAGGACTACACAAGGGTTGTGTGCCCTGTGATTGACATCATTAACCTGGATACCTTCAACTATATTGAGTCTGCCTCAGAGCTCAGAGGGGGCTTTGACTGGAGCCTGCACTTCCAGTGGGAGCAGCTCTCCCTGGAGCAGAAAGCTTTGCGCCTAGACCCTACTGAGCCCATCCGATGTTCGCTGCTTGTGTATTGCACGGACCTTCCTCCCACCAGCATCATCATCACCTTCCACAACGAGGCACGTTCCACTCTACTCAGAACCATCCGCAGTGTGTTAAACCGAACCCCTATGCATCTGATCCAGGAAATCATATTAGTGGATGACTTTAGCAATGACCCCGAGGACTGCAAGCAACTCATCAAATTGCCCAAGGTGAAGTGCTTGCGCAACAATGAGCGGCAAGGTCTGGTCCGGTCTCGGATGCGAGGTGCTGACATTGCCCAAGGCACCACTCTGACCTTCCTGGACAGCCATTGTGAGGTGAACAGGGATTGGCTGCAGCCCCTGTTACACAGGGTCAAAGAGGACTACACAAGGGTTGTGTGCCCTGTGATTGACATCATTAACCTGGATACCTTCAACTATATTGAGTCTGCCTCAGAGCTCAGAGGGGGCTTTGACTGGAGCCTGCACTTCCAGTGGGAGCAGCTCTCCCTGGAGCAGAAAGCTTTGCGCCTAGACCCTACTGAGCCCATC
Peptide sequence:
MHLIQEIILVDDFSNDPEDCKQLIKLPKVKCLRNNERQGLVRSRMRGADIAQGTTLTFLDSHCEVNRDWLQPLLHRVKEDYTRVVCPVIDIINLDTFNYIESASELRGGFDWSLHFQWEQLSLEQKALRLDPTEPIRCSLLVYCTDLPPTSIIITFHNEARSTLLRTIRSVLNRTPMHLIQEIILVDDFSNDPEDCKQLIKLPKVKCLRNNERQGLVRSRMRGADIAQGTTLTFLDSHCEVNRDWLQPLLHRVKEDYTRVVCPVIDIINLDTFNYIESASELRGGFDWSLHFQWEQLSLEQKALRLDPTEPI
IRESfinder result
IRESfinder score
0.557021464
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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