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mmu_circG3bp1_195
Basic information of circular RNA
PanCircBase ID:
mmu_circG3bp1_195
mm39_Chr. Position ID:
chr11|55376451|55378386|+
Gene name:
G3bp1
Transcript:
ENSMUST00000018727.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,1853
Genomic length:
1935
Exon/intron sizes in nt:
113,82
Flanking exon/introns:
chr11:55360627-55376451|chr11:55378386-55379844
Splice length:
195
Other database IDs:
CIRCpedia: MMU_CIRCpedia_212386, circAtlas: mmu-G3bp1_0004
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_179060
Human Ortholog IDs:
Mature spliced Sequence:
GTTGAACCCACCAAAGCGATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACTCTGCTGAACCAGGCCCCGGACATGTTGCACAGATTTTATGGAAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTCCAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAGCAGATGCAGTCTAC
59.82
GCCTGGTTCAGCAGAGTGTA
59.68
124
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGCCAGCAGATGCAGTCT
60.39
GCCTGGTTCAGCAGAGTGTA
59.68
126
PCR template sequence:
GGACATGTTGCACAGATTTTATGGAAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTCCAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAGGTTGAACCCACCAAAGCGATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACTCTGCTGAACCAGGCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CTACGGGCAGAAGGTTGAACCCACCA
siRNA sets
TTCAACCTTCTGCCCGTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCG3BP1_195_ORF_1
0.9406
0.426348128
0.715968235
372
ORF sequence:
ATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACTCTGCTGAACCAGGCCCCGGACATGTTGCACAGATTTTATGGAAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTCCAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAGGTTGAACCCACCAAAGCGATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACTCTGCTGAACCAGGCCCCGGACATGTTGCACAGATTTTATGGAAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTCCAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAG
Peptide sequence:
MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGLDSNGKPADAVYGQKVEPTKAMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGLDSNGKPADAVYGQK
IRESfinder result
IRESfinder score
0.29395672
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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