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mmu_circFryl_924_1
Basic information of circular RNA
PanCircBase ID:
mmu_circFryl_924_1
mm39_Chr. Position ID:
chr5|73211395|73216892|-
Gene name:
Fryl
Transcript:
ENSMUST00000202697.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
11,10,9,8,7,6
Exon/intron offsets:
0,477,663,1472,3671,5326
Genomic length:
5497
Exon/intron sizes in nt:
17,77,51,62,19,81,41,100
Flanking exon/introns:
chr5:73207848-73211395|chr5:73216892-73221848
Splice length:
924
Other database IDs:
circAtlas: mmu-Fryl_0029
CIRCpedia: HSA_CIRCpedia_356020
Human Ortholog IDs:
Mature spliced Sequence:
GTCTGTGCGGAAGAGAAGTGCCCGACGCTGGTGAACCTGGCTCACATGATGAGTTTGTACAGCACACATACCTACTCCAGAGACTGTTCCAACTGGATCAACGTGGTGTGCCGGTACCTGCACGACTCTTTCTCAGAGGCGACCTTCAGTCTTGTGACTTACCTGGCAGAGCTGTTAGAAAAGGGACTGTCCAGCATGCAGCAGTCGTTGCTACAGATCATCTACAGTCTGTTGAGTCACATCGACTTGTCTGCGGCCCCGGTCAAGCAGTTTAATCTGGAGATCATAAAGATCATTGGCAAATACGTACAGAGCCCGTACTGGAAGGAAGCCCTCAACATCCTGAAGCTGGTGGTGTCTCGCTCTGCAAGTCTTGTCGTCCCCAATGACATCCCCAAGGCCTACGGAGTGGACGTAGGCTCTCCCGAAATCTCCTTCGCTAAAATTTTCAATAACGTCTCGAAGGAGCTGCCCGGCAAGACCTTAGATTTTCACTTTGACATCTCTGAGACACCTATCATTGGGAACAAGTATGGAGGCCAACACAGTGCCGCGGGAAGGAATGGGAAGGCAAAGGTGATCGCAGTCACCAGGAGCACGTCCTCCACCTCCTCTGGCTCCACATCCAACGCCCTAGTTCCTGTCAGCTGGAAAAGGCCACAGTTATCACAGCGGAGAACGAGAGAGAAGCTGATGAGCGTGCTTTCCCTCTGTGGCCCCGAATCTGGCCTCCCCAAGAATCCCTCCGTTGTGTTTTCTTCAAATGAGGATTTGGAAGTCGGGGACCAACAGACCAGCTTGATCTCCACCACCGAGGACATAATTCAAGAAGAAGAAGTTGCCGTCGAAGACAACAGCAGTGAGCAGCAGTTTGGCGTCTTCAAGGACTTCGACTTCTTGGATGTTGAATTGGAGGATGCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGAAGTTGCCGTCGAAGA
59.66
TCATGTGAGCCAGGTTCACC
59.96
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGAAGAAGTTGCCGTCGA
59.66
TCATGTGAGCCAGGTTCACC
59.96
146
PCR template sequence:
TCAAGAAGAAGAAGTTGCCGTCGAAGACAACAGCAGTGAGCAGCAGTTTGGCGTCTTCAAGGACTTCGACTTCTTGGATGTTGAATTGGAGGATGCAGAGGTCTGTGCGGAAGAGAAGTGCCCGACGCTGGTGAACCTGGCTCACATGATGAGTTTGTACAGCACACATACCTACTCCAGAGACTGTTCCAACTGGATCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-431-5p(1), mmu-miR-212-3p(1), mmu-miR-541-5p(1), mmu-miR-297a-5p(1), mmu-miR-499-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAGGATGCAGAGGTCTGTGCGGAAG
siRNA sets
CTTCCGCACAGACCTCTGC, TTCCGCACAGACCTCTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRYL_924_1_ORF_1
1.2811
0.372384956
0.999991102
1803
ORF sequence:
ATGATGAGTTTGTACAGCACACATACCTACTCCAGAGACTGTTCCAACTGGATCAACGTGGTGTGCCGGTACCTGCACGACTCTTTCTCAGAGGCGACCTTCAGTCTTGTGACTTACCTGGCAGAGCTGTTAGAAAAGGGACTGTCCAGCATGCAGCAGTCGTTGCTACAGATCATCTACAGTCTGTTGAGTCACATCGACTTGTCTGCGGCCCCGGTCAAGCAGTTTAATCTGGAGATCATAAAGATCATTGGCAAATACGTACAGAGCCCGTACTGGAAGGAAGCCCTCAACATCCTGAAGCTGGTGGTGTCTCGCTCTGCAAGTCTTGTCGTCCCCAATGACATCCCCAAGGCCTACGGAGTGGACGTAGGCTCTCCCGAAATCTCCTTCGCTAAAATTTTCAATAACGTCTCGAAGGAGCTGCCCGGCAAGACCTTAGATTTTCACTTTGACATCTCTGAGACACCTATCATTGGGAACAAGTATGGAGGCCAACACAGTGCCGCGGGAAGGAATGGGAAGGCAAAGGTGATCGCAGTCACCAGGAGCACGTCCTCCACCTCCTCTGGCTCCACATCCAACGCCCTAGTTCCTGTCAGCTGGAAAAGGCCACAGTTATCACAGCGGAGAACGAGAGAGAAGCTGATGAGCGTGCTTTCCCTCTGTGGCCCCGAATCTGGCCTCCCCAAGAATCCCTCCGTTGTGTTTTCTTCAAATGAGGATTTGGAAGTCGGGGACCAACAGACCAGCTTGATCTCCACCACCGAGGACATAATTCAAGAAGAAGAAGTTGCCGTCGAAGACAACAGCAGTGAGCAGCAGTTTGGCGTCTTCAAGGACTTCGACTTCTTGGATGTTGAATTGGAGGATGCAGAGGTCTGTGCGGAAGAGAAGTGCCCGACGCTGGTGAACCTGGCTCACATGATGAGTTTGTACAGCACACATACCTACTCCAGAGACTGTTCCAACTGGATCAACGTGGTGTGCCGGTACCTGCACGACTCTTTCTCAGAGGCGACCTTCAGTCTTGTGACTTACCTGGCAGAGCTGTTAGAAAAGGGACTGTCCAGCATGCAGCAGTCGTTGCTACAGATCATCTACAGTCTGTTGAGTCACATCGACTTGTCTGCGGCCCCGGTCAAGCAGTTTAATCTGGAGATCATAAAGATCATTGGCAAATACGTACAGAGCCCGTACTGGAAGGAAGCCCTCAACATCCTGAAGCTGGTGGTGTCTCGCTCTGCAAGTCTTGTCGTCCCCAATGACATCCCCAAGGCCTACGGAGTGGACGTAGGCTCTCCCGAAATCTCCTTCGCTAAAATTTTCAATAACGTCTCGAAGGAGCTGCCCGGCAAGACCTTAGATTTTCACTTTGACATCTCTGAGACACCTATCATTGGGAACAAGTATGGAGGCCAACACAGTGCCGCGGGAAGGAATGGGAAGGCAAAGGTGATCGCAGTCACCAGGAGCACGTCCTCCACCTCCTCTGGCTCCACATCCAACGCCCTAGTTCCTGTCAGCTGGAAAAGGCCACAGTTATCACAGCGGAGAACGAGAGAGAAGCTGATGAGCGTGCTTTCCCTCTGTGGCCCCGAATCTGGCCTCCCCAAGAATCCCTCCGTTGTGTTTTCTTCAAATGAGGATTTGGAAGTCGGGGACCAACAGACCAGCTTGATCTCCACCACCGAGGACATAATTCAAGAAGAAGAAGTTGCCGTCGAAGACAACAGCAGTGAGCAGCAGTTTGGCGTCTTCAAGGACTTCGACTTCTTGGATGTTGAATTGGAGGATGCAGAG
Peptide sequence:
MMSLYSTHTYSRDCSNWINVVCRYLHDSFSEATFSLVTYLAELLEKGLSSMQQSLLQIIYSLLSHIDLSAAPVKQFNLEIIKIIGKYVQSPYWKEALNILKLVVSRSASLVVPNDIPKAYGVDVGSPEISFAKIFNNVSKELPGKTLDFHFDISETPIIGNKYGGQHSAAGRNGKAKVIAVTRSTSSTSSGSTSNALVPVSWKRPQLSQRRTREKLMSVLSLCGPESGLPKNPSVVFSSNEDLEVGDQQTSLISTTEDIIQEEEVAVEDNSSEQQFGVFKDFDFLDVELEDAEVCAEEKCPTLVNLAHMMSLYSTHTYSRDCSNWINVVCRYLHDSFSEATFSLVTYLAELLEKGLSSMQQSLLQIIYSLLSHIDLSAAPVKQFNLEIIKIIGKYVQSPYWKEALNILKLVVSRSASLVVPNDIPKAYGVDVGSPEISFAKIFNNVSKELPGKTLDFHFDISETPIIGNKYGGQHSAAGRNGKAKVIAVTRSTSSTSSGSTSNALVPVSWKRPQLSQRRTREKLMSVLSLCGPESGLPKNPSVVFSSNEDLEVGDQQTSLISTTEDIIQEEEVAVEDNSSEQQFGVFKDFDFLDVELEDAE
IRESfinder result
IRESfinder score
0.530299143
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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