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mmu_circFryl_706
Basic information of circular RNA
PanCircBase ID:
mmu_circFryl_706
mm39_Chr. Position ID:
chr5|73221848|73222889|-
Gene name:
Fryl
Transcript:
ENSMUST00000202697.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,4
Exon/intron offsets:
0,433
Genomic length:
1041
Exon/intron sizes in nt:
98,608
Flanking exon/introns:
chr5:73216892-73221848|chr5:73222889-73224616
Splice length:
706
Other database IDs:
circAtlas: mmu-Fryl_0067
Human Ortholog IDs:
Mature spliced Sequence:
ATCCTCATACCACGATCCTGTCATGGGAAACAAGTATGCAGCTAACAGGAAAAGCACTGGCCAGCTCAATCTAAGCACAAGTCCCATTAACAGCAGCGGCCATTTGGGATGCAACGGGGACACAAGAAGTAACTCTTTGAGGTTAAGTCTAGTTGGTGACCGCAGAGGTGACCGGCGGCGGAGTAACACACTGGACATAACGGATGGACGGATAAACCACGGTGGTAGCTTGGCGAGGACTCGAAGCCTTTCCTCGCTGAGAGAGAAAGGTGTGTACGACACGCAGCCCCCGACGGAGCCGAGCAACCTGATGGCCACCATCTTTTGGATAGCAACATCTCTGTTAGAATCCGACTATGAATATGAATACCTCCTGGCTCTCAGGCTTCTGAGCAAACTGCTCACCCACTTGCCTCTGGATAAGTCAGAGAGCCGGGAGAAGATCGAGAACGTACAGAGCAAGTTGAAATGGAGTAACTTCCCCGGCCTTCAGCAGCTCTTCCTTAAGGGCTTTACCTCAGTGTCTACCCAAGAGATGACCGTGCACCTCCTCAGTCAGCTCATTTCCGTCTCCAAACACACACTGGTGGATCCGTCCCAGGTGTCAGGCTTTCCTCTTAACATCCTCTGCTTACTGCCTCACCTAATCCAGCATTTTGACAGCCCAACTCAGTTTTGCAAAGAGACAGCTAGCCGGATAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCTGCTTACTGCCTCACC
60.11
TAGATTGAGCTGGCCAGTGC
60.11
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCTGCTTACTGCCTCACC
60.11
TGGCCAGTGCTTTTCCTGTT
60.4
145
PCR template sequence:
AGGCTTTCCTCTTAACATCCTCTGCTTACTGCCTCACCTAATCCAGCATTTTGACAGCCCAACTCAGTTTTGCAAAGAGACAGCTAGCCGGATAGCAAAGATCCTCATACCACGATCCTGTCATGGGAAACAAGTATGCAGCTAACAGGAAAAGCACTGGCCAGCTCAATCTAAGCACAAGTCCCATTAACAGCAGCGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-136-5p(1), mmu-miR-127-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CCGGATAGCAAAGATCCTCATACCAC
siRNA sets
TATGAGGATCTTTGCTATC, GTGGTATGAGGATCTTTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRYL_706_ORF_1
0.9901
0.18742315
0.930352114
729
ORF sequence:
ATGGGAAACAAGTATGCAGCTAACAGGAAAAGCACTGGCCAGCTCAATCTAAGCACAAGTCCCATTAACAGCAGCGGCCATTTGGGATGCAACGGGGACACAAGAAGTAACTCTTTGAGGTTAAGTCTAGTTGGTGACCGCAGAGGTGACCGGCGGCGGAGTAACACACTGGACATAACGGATGGACGGATAAACCACGGTGGTAGCTTGGCGAGGACTCGAAGCCTTTCCTCGCTGAGAGAGAAAGGTGTGTACGACACGCAGCCCCCGACGGAGCCGAGCAACCTGATGGCCACCATCTTTTGGATAGCAACATCTCTGTTAGAATCCGACTATGAATATGAATACCTCCTGGCTCTCAGGCTTCTGAGCAAACTGCTCACCCACTTGCCTCTGGATAAGTCAGAGAGCCGGGAGAAGATCGAGAACGTACAGAGCAAGTTGAAATGGAGTAACTTCCCCGGCCTTCAGCAGCTCTTCCTTAAGGGCTTTACCTCAGTGTCTACCCAAGAGATGACCGTGCACCTCCTCAGTCAGCTCATTTCCGTCTCCAAACACACACTGGTGGATCCGTCCCAGGTGTCAGGCTTTCCTCTTAACATCCTCTGCTTACTGCCTCACCTAATCCAGCATTTTGACAGCCCAACTCAGTTTTGCAAAGAGACAGCTAGCCGGATAGCAAAGATCCTCATACCACGATCCTGTCATGGGAAACAAGTATGCAGCTAA
Peptide sequence:
MGNKYAANRKSTGQLNLSTSPINSSGHLGCNGDTRSNSLRLSLVGDRRGDRRRSNTLDITDGRINHGGSLARTRSLSSLREKGVYDTQPPTEPSNLMATIFWIATSLLESDYEYEYLLALRLLSKLLTHLPLDKSESREKIENVQSKLKWSNFPGLQQLFLKGFTSVSTQEMTVHLLSQLISVSKHTLVDPSQVSGFPLNILCLLPHLIQHFDSPTQFCKETASRIAKILIPRSCHGKQVCS
IRESfinder result
IRESfinder score
0.476588634
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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