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mmu_circFryl_2632
Basic information of circular RNA
PanCircBase ID:
mmu_circFryl_2632
mm39_Chr. Position ID:
chr5|73231184|73255770|-
Gene name:
Fryl
Transcript:
ENSMUST00000101127.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
18
Exon/intron indexes:
40,39,38,37,36,35,34,33,32,31,30,29,28,27,26,25,24,23
Exon/intron offsets:
0,787,2046,2816,7122,8955,9286,11505,12685,13036,14527,15253,16759,19281,21574,22432,23532,24317
Genomic length:
24586
Exon/intron sizes in nt:
10,41,51,11,38,51,69,10,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr5:73230264-73231184|chr5:73255770-73257288
Splice length:
2632
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACTACTTTGCTGTATTGCCCTAGCTCTGTAGATCTGCAGACATTAGCAGATTGGAACTCTTCTCCCATCAGCCACCAGTTTGATGTGATCAGCCCCTCACACATTTGGATATTTGCACATGTGACCCAAGGCCAAGACCCATGGATTATAAGTCTCTCCAGTTTCCTAAAGCAAGAAAACCTTCCCAAACACTGCTCTACAGCCGTGAGCTATGCGTGGATGTTCGCATACACCAGGCTGCAGCTCTTGTCCCCCCAGGTGGATATAAATAGCCCCATCAATGCTAAGAAAGTAAATGCCACCACGAGCAGCGACTCGTACATTGGCCTGTGGAGAAACTACCTGGTCCTCTGCTGCAGCGCAGCCACCTCCCCGTCCCCATCAGCACCCGCAGGCTCCGTGAGATGCTCTCCTCCCGAGACGCTGGCGTCCACCCCTGACAGTGGCTACAGCATCGATTCCAAGATCGTTGGCATCCCGTCCCCTTCTTCCCTGTTTAAGCACATAGTTCCAATGATGCGCTCTGAGAGCATGGAGATCACAGAGTCCCTCGTTCTAGGCCTCGGCAGGACCAACCCGGGAGTCTTTAGAGAATTAATAGAGGAGTTGCATCCCATCATTAAAGAAGCACTTGAAAGAAGGCCAGAGAATATGAAACGGCGCAGGCGTCGAGACATTTTACGAGTACAGCTGGTACGGATATTTGAGCTGTTGGCGGATGCTGGTGTCATTAGCCACAGTGCAAGCGGCGGCCTTGATAGCGAAACTCACTTCCTCAACAACACTCTCTTGGAGTACGTGGACTTAACGCGACAACTTCTGGAAGCAGAAAACGAGAAGGACTCCGACACCCTGAAGGATATCCGATGCCATTTCAGTGCCTTAGTGGCGAATATCATTCAGAATGTTCCGGTGCACCAGAGGAGAAGCATCTTTCCCCAGCAGAGCCTGCGTCACAGCCTGTTCATGTTGTTCAGCCACTGGGCGGGCCCCTTTAGCATCATGTTCACGCCCCTGGACAGATACAGTGACAGGAATATGCAGATCAACAGACACCAGTACTGTGCGTTAAAGGCTATGTCTGCTGTACTGTGTTGTGGCCCTGTTGCAGATAATGTGGGACTGTCATCAGATGGCTATTTGTACAAATGGCTGGATAACATTTTGGACTCTCTGGATAAAAAGGTTCACCAGTTGGGCTGTGAAGCAGTCACGTTGCTGCTGGAGCTGAACCCTGATCAGAGCAGCCTGATGTACTGGGCCGTGGACCGATGCTACACCGGCTCCCGGAGAGTAGCAGCCGGCTGCTTCAAAGCCATTGCCAACGTGTTCCAGAACAGGGATTACCAGTGTGACACAGTGATGCTCCTAAACCTGATACTGTTTAAAGCAGCTGACTCTTCTAGAAGTATCTATGAAGTTGCTATGCAACTCTTGCAGATCCTGGAGCCGAAGATGTTTCGCTATGCTCACAAACTGGAGGTTCAGAGAACGGATGGAGTCCTCAGTCAGCTGTCCCCGCTGCCACATCTCTATTCTGTGTCTTATTACCAGTTGTCTGAGGAGCTAGCCCGAGCATATCCAGAGCTCACTCTCGCCATATTCTCAGAGATCAGCCAGCGGATCCAGACGGCACACCCCGCGGGGCGGCAGGCCATGCTTCATTACCTACTGCCCTGGATGAACAACATCGAGCTGGTCGACCTCAAACCTCTGCCTTCAGGGAGGCGGCAGGATGAAGATGAAGATGACTCCTTGAAAGACCGAGAGCTCATGGTGACCAGCAGGCGCTGGCTGAGAGGAGAAGGCTGGGGCTCCCCACAGGCCACCGCCATGGTGTTGAACAATCTCATGTACATGACAGCGAAGTACGGCGATGAGCTGGCCTGGTCGGAGGTGGAGAATGTGTGGACCACCCTTGCAGACGGCTGGCCAAAGAACCTGAAAATTATTTTGCACTTTCTGATCAGCATCTGCGGAGTGAATAGCGAACCAAGCCTCTTGCCTTACGTGAAGAAAGTCATCGTCTACCTGGGTCGAGACAAAACCATGCAGCTGCTGGAAGAGCTGGTGAGCGAGCTGCAGCTCACGGACCCCGTCAGTTCCGGCGTCACGCACATGGATAACCCGCCCTACTACCGCATCACTTCCAGCTGTAAAATCCCTTCCGTCACCTCAGGAACTACTTCCAGTAGCAATACAATGGTCGCTCCCACAGATGGCAACCCTGACAGTAAGGCCCTAAAGGAGAACTTTGAAGAGAGCTATGTGCACCTGGACATCTACGGGGGACTGAACAGTCATTTAAACCGGCAACACCACAGACTGGAGTCCCGATACAGTAGCAGCTCCGGAGGGTCTTACGAAGAGGAGAAAAGTGACTCAATGCCACTTTATTCTAATTGGCGCCTGAAAGTGATGGAGCACAACCAAGGAGAGCCACTGCCCTTCCCGCCAGCTGGAGGCTGCTGGTCGCCACTGGTGGATTACGTGCCTGAAACAGCATCTCCTGGATTGCCTCTACACAGGTGTAACATAGCCGTGATCCTGCTGACAGATCTGATCATTGACCACAGCGTGAAGGTGGAATGGGGAAGTTACCTCCACCTGCTGCTTCATGCAATTTTTATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATAGCCGTGATCCTGCTG
59.89
AAACTGGTGGCTGATGGGAG
59.96
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGACCACAGCGTGAAGGT
59.89
AAACTGGTGGCTGATGGGAG
59.96
148
PCR template sequence:
AACATAGCCGTGATCCTGCTGACAGATCTGATCATTGACCACAGCGTGAAGGTGGAATGGGGAAGTTACCTCCACCTGCTGCTTCATGCAATTTTTATAGACTACTTTGCTGTATTGCCCTAGCTCTGTAGATCTGCAGACATTAGCAGATTGGAACTCTTCTCCCATCAGCCACCAGTTTGATGTGATCAGCCCCTCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
31
Interacting miRNAs (#binding sites)
mmu-miR-101a-3p(2), mmu-miR-210-3p(1), mmu-miR-20b-5p(1), mmu-miR-134-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-106a-5p(1), mmu-miR-20a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-31-5p(1), mmu-miR-19b-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-19a-3p(1), mmu-miR-298-5p(1), mmu-miR-196a-5p(1), mmu-miR-24-3p(1), mmu-miR-503-5p(1), mmu-miR-182-5p(1), mmu-miR-301b-3p(1), mmu-miR-382-5p(1), mmu-miR-541-5p(1), mmu-miR-425-5p(1), mmu-miR-297a-5p(1), mmu-miR-301a-3p(1), mmu-miR-383-5p(1), mmu-miR-673-5p(1), mmu-miR-670-5p(1), mmu-miR-1904(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAATTTTTATAGACTACTTTGCTGT
siRNA sets
AAGTAGTCTATAAAAATTG, AAAGTAGTCTATAAAAATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRYL_2632_ORF_1
1.0453
0.325653697
0.999999812
2436
ORF sequence:
ATGTTCGCATACACCAGGCTGCAGCTCTTGTCCCCCCAGGTGGATATAAATAGCCCCATCAATGCTAAGAAAGTAAATGCCACCACGAGCAGCGACTCGTACATTGGCCTGTGGAGAAACTACCTGGTCCTCTGCTGCAGCGCAGCCACCTCCCCGTCCCCATCAGCACCCGCAGGCTCCGTGAGATGCTCTCCTCCCGAGACGCTGGCGTCCACCCCTGACAGTGGCTACAGCATCGATTCCAAGATCGTTGGCATCCCGTCCCCTTCTTCCCTGTTTAAGCACATAGTTCCAATGATGCGCTCTGAGAGCATGGAGATCACAGAGTCCCTCGTTCTAGGCCTCGGCAGGACCAACCCGGGAGTCTTTAGAGAATTAATAGAGGAGTTGCATCCCATCATTAAAGAAGCACTTGAAAGAAGGCCAGAGAATATGAAACGGCGCAGGCGTCGAGACATTTTACGAGTACAGCTGGTACGGATATTTGAGCTGTTGGCGGATGCTGGTGTCATTAGCCACAGTGCAAGCGGCGGCCTTGATAGCGAAACTCACTTCCTCAACAACACTCTCTTGGAGTACGTGGACTTAACGCGACAACTTCTGGAAGCAGAAAACGAGAAGGACTCCGACACCCTGAAGGATATCCGATGCCATTTCAGTGCCTTAGTGGCGAATATCATTCAGAATGTTCCGGTGCACCAGAGGAGAAGCATCTTTCCCCAGCAGAGCCTGCGTCACAGCCTGTTCATGTTGTTCAGCCACTGGGCGGGCCCCTTTAGCATCATGTTCACGCCCCTGGACAGATACAGTGACAGGAATATGCAGATCAACAGACACCAGTACTGTGCGTTAAAGGCTATGTCTGCTGTACTGTGTTGTGGCCCTGTTGCAGATAATGTGGGACTGTCATCAGATGGCTATTTGTACAAATGGCTGGATAACATTTTGGACTCTCTGGATAAAAAGGTTCACCAGTTGGGCTGTGAAGCAGTCACGTTGCTGCTGGAGCTGAACCCTGATCAGAGCAGCCTGATGTACTGGGCCGTGGACCGATGCTACACCGGCTCCCGGAGAGTAGCAGCCGGCTGCTTCAAAGCCATTGCCAACGTGTTCCAGAACAGGGATTACCAGTGTGACACAGTGATGCTCCTAAACCTGATACTGTTTAAAGCAGCTGACTCTTCTAGAAGTATCTATGAAGTTGCTATGCAACTCTTGCAGATCCTGGAGCCGAAGATGTTTCGCTATGCTCACAAACTGGAGGTTCAGAGAACGGATGGAGTCCTCAGTCAGCTGTCCCCGCTGCCACATCTCTATTCTGTGTCTTATTACCAGTTGTCTGAGGAGCTAGCCCGAGCATATCCAGAGCTCACTCTCGCCATATTCTCAGAGATCAGCCAGCGGATCCAGACGGCACACCCCGCGGGGCGGCAGGCCATGCTTCATTACCTACTGCCCTGGATGAACAACATCGAGCTGGTCGACCTCAAACCTCTGCCTTCAGGGAGGCGGCAGGATGAAGATGAAGATGACTCCTTGAAAGACCGAGAGCTCATGGTGACCAGCAGGCGCTGGCTGAGAGGAGAAGGCTGGGGCTCCCCACAGGCCACCGCCATGGTGTTGAACAATCTCATGTACATGACAGCGAAGTACGGCGATGAGCTGGCCTGGTCGGAGGTGGAGAATGTGTGGACCACCCTTGCAGACGGCTGGCCAAAGAACCTGAAAATTATTTTGCACTTTCTGATCAGCATCTGCGGAGTGAATAGCGAACCAAGCCTCTTGCCTTACGTGAAGAAAGTCATCGTCTACCTGGGTCGAGACAAAACCATGCAGCTGCTGGAAGAGCTGGTGAGCGAGCTGCAGCTCACGGACCCCGTCAGTTCCGGCGTCACGCACATGGATAACCCGCCCTACTACCGCATCACTTCCAGCTGTAAAATCCCTTCCGTCACCTCAGGAACTACTTCCAGTAGCAATACAATGGTCGCTCCCACAGATGGCAACCCTGACAGTAAGGCCCTAAAGGAGAACTTTGAAGAGAGCTATGTGCACCTGGACATCTACGGGGGACTGAACAGTCATTTAAACCGGCAACACCACAGACTGGAGTCCCGATACAGTAGCAGCTCCGGAGGGTCTTACGAAGAGGAGAAAAGTGACTCAATGCCACTTTATTCTAATTGGCGCCTGAAAGTGATGGAGCACAACCAAGGAGAGCCACTGCCCTTCCCGCCAGCTGGAGGCTGCTGGTCGCCACTGGTGGATTACGTGCCTGAAACAGCATCTCCTGGATTGCCTCTACACAGGTGTAACATAGCCGTGATCCTGCTGACAGATCTGATCATTGACCACAGCGTGAAGGTGGAATGGGGAAGTTACCTCCACCTGCTGCTTCATGCAATTTTTATAGACTACTTTGCTGTATTGCCCTAG
Peptide sequence:
MFAYTRLQLLSPQVDINSPINAKKVNATTSSDSYIGLWRNYLVLCCSAATSPSPSAPAGSVRCSPPETLASTPDSGYSIDSKIVGIPSPSSLFKHIVPMMRSESMEITESLVLGLGRTNPGVFRELIEELHPIIKEALERRPENMKRRRRRDILRVQLVRIFELLADAGVISHSASGGLDSETHFLNNTLLEYVDLTRQLLEAENEKDSDTLKDIRCHFSALVANIIQNVPVHQRRSIFPQQSLRHSLFMLFSHWAGPFSIMFTPLDRYSDRNMQINRHQYCALKAMSAVLCCGPVADNVGLSSDGYLYKWLDNILDSLDKKVHQLGCEAVTLLLELNPDQSSLMYWAVDRCYTGSRRVAAGCFKAIANVFQNRDYQCDTVMLLNLILFKAADSSRSIYEVAMQLLQILEPKMFRYAHKLEVQRTDGVLSQLSPLPHLYSVSYYQLSEELARAYPELTLAIFSEISQRIQTAHPAGRQAMLHYLLPWMNNIELVDLKPLPSGRRQDEDEDDSLKDRELMVTSRRWLRGEGWGSPQATAMVLNNLMYMTAKYGDELAWSEVENVWTTLADGWPKNLKIILHFLISICGVNSEPSLLPYVKKVIVYLGRDKTMQLLEELVSELQLTDPVSSGVTHMDNPPYYRITSSCKIPSVTSGTTSSSNTMVAPTDGNPDSKALKENFEESYVHLDIYGGLNSHLNRQHHRLESRYSSSSGGSYEEEKSDSMPLYSNWRLKVMEHNQGEPLPFPPAGGCWSPLVDYVPETASPGLPLHRCNIAVILLTDLIIDHSVKVEWGSYLHLLLHAIFIDYFAVLP
IRESfinder result
IRESfinder score
0.459719128
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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