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mmu_circFryl_1076
Basic information of circular RNA
PanCircBase ID:
mmu_circFryl_1076
mm39_Chr. Position ID:
chr5|73179844|73198936|-
Gene name:
Fryl
Transcript:
ENSMUST00000202697.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
20,19,18,17,16,15,14
Exon/intron offsets:
0,1386,4814,10256,11094,12444,18844
Genomic length:
19092
Exon/intron sizes in nt:
19,11,11,69,99,15,02,00,000
Flanking exon/introns:
chr5:73179640-73179844|chr5:73198936-73204862
Splice length:
1076
Other database IDs:
CIRCpedia: MMU_CIRCpedia_28260, circAtlas: mmu-Fryl_0062
Human Ortholog IDs:
Mature spliced Sequence:
GGTACAAGTTCAGTATTAAAGGAGGAACACGTTACAGCCTTTGAAGACGAAGGGTCATACGTTATTCCAGACCAACAGGACCCGCTAGTATGTCGAGGGATTCTTGATTTAGAGGAAACTGACATGCCAGAGCCTCTGGCCCCTGAAAGCTACCCCGAGTCCATCTGTGAAGAGGATGTCACCTTGGCTTTGAAAGAGCTGGATGAACGGTGTGAGGAGGAAGAGGCTGACTTCTCAGGGTTGTCCAGTCAAGAGGAAGATGAACAAGACGGCTTTCCAGAAGTGCAAACCTCGCCTCTCCCGACCCCCTTTCTGTCTGCCATAATAGCAGCTTTCCAGCCAATGGCTTACGAGGACGAGGAAGAAGCGTGGCGTTGCCATGTCAATCAGACGCTGTCGGACACAGACGGGTCCTGTGCGGTGTTTACTTTCCACGTGTTTTCAAGACTGTTTCAGACAATTCAAAGGAAGTTTGGAGAGATAACGAAGGAGGCTGTGGGCTTTCTTGGTGAGAGTCTACAGCGCATTGGTACCAAGTTTAAAAGCTCATTGGAAGTGATGATGGCCTGTTCTGAATGCCCCACTGTCTTTGTGGATGCCGAAACGCTGATGGCGTGTGGCTTGCTGGAAACACTCAAGTTTGGCGTCTTGGAGCTGCAGGAACATCTGGATACTTACACCACCAAGAGAGAAGCAGCCGAGCAGTGGCTAGACAACTGCAAGAGGACATTCGGTGCCAACGAAGACATATACAGGATGAACACCAACGCGCATGAGCTGGAGCTCTGCAGAAGGTTGTACCGGCTGCACTTTCAGCTGCTGCTTCTGTTCCAGGCCTACTGCAAACTCATCAACCAAGTAAATACCATCAAGAATGAAGCAGAGGTCATCAACATGTCAGAAGAACTTGCCCAGCTGGAAGGCATCCTTAAGGAAGCTGAGGCCGCTTCGGAAAATGAAGAAATTGATATCTCCAAAGCGGCCCAGACCACGATAGAAACTGCCATTCACTCTCTGATCGAAACCTTGAAGAACAAGGAATTTGTATCAGCTGTGGCACAAGTCAAAGCTTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCCAGACCACGATAGAAA
59.46
GGGTCCTGTTGGTCTGGAAT
59.3
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCCAGACCACGATAGAAA
59.46
ACCCTTCGTCTTCAAAGGCT
59.23
151
PCR template sequence:
AAGCGGCCCAGACCACGATAGAAACTGCCATTCACTCTCTGATCGAAACCTTGAAGAACAAGGAATTTGTATCAGCTGTGGCACAAGTCAAAGCTTTCAGGGTACAAGTTCAGTATTAAAGGAGGAACACGTTACAGCCTTTGAAGACGAAGGGTCATACGTTATTCCAGACCAACAGGACCCGCTAGTATGTCGAGGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
23
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-223-3p(1), mmu-miR-30e-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-18a-5p(1), mmu-miR-132-3p(1), mmu-miR-125b-5p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-351-5p(1), mmu-miR-125a-5p(1), mmu-miR-212-3p(1), mmu-miR-383-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
TCAAAGCTTTCAGGGTACAAGTTCAG
siRNA sets
GAACTTGTACCCTGAAAGC, AACTTGTACCCTGAAAGCT, TGTACCCTGAAAGCTTTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRYL_1076_ORF_1
1.0635
0.341010538
0.993896904
972
ORF sequence:
ATGCCAGAGCCTCTGGCCCCTGAAAGCTACCCCGAGTCCATCTGTGAAGAGGATGTCACCTTGGCTTTGAAAGAGCTGGATGAACGGTGTGAGGAGGAAGAGGCTGACTTCTCAGGGTTGTCCAGTCAAGAGGAAGATGAACAAGACGGCTTTCCAGAAGTGCAAACCTCGCCTCTCCCGACCCCCTTTCTGTCTGCCATAATAGCAGCTTTCCAGCCAATGGCTTACGAGGACGAGGAAGAAGCGTGGCGTTGCCATGTCAATCAGACGCTGTCGGACACAGACGGGTCCTGTGCGGTGTTTACTTTCCACGTGTTTTCAAGACTGTTTCAGACAATTCAAAGGAAGTTTGGAGAGATAACGAAGGAGGCTGTGGGCTTTCTTGGTGAGAGTCTACAGCGCATTGGTACCAAGTTTAAAAGCTCATTGGAAGTGATGATGGCCTGTTCTGAATGCCCCACTGTCTTTGTGGATGCCGAAACGCTGATGGCGTGTGGCTTGCTGGAAACACTCAAGTTTGGCGTCTTGGAGCTGCAGGAACATCTGGATACTTACACCACCAAGAGAGAAGCAGCCGAGCAGTGGCTAGACAACTGCAAGAGGACATTCGGTGCCAACGAAGACATATACAGGATGAACACCAACGCGCATGAGCTGGAGCTCTGCAGAAGGTTGTACCGGCTGCACTTTCAGCTGCTGCTTCTGTTCCAGGCCTACTGCAAACTCATCAACCAAGTAAATACCATCAAGAATGAAGCAGAGGTCATCAACATGTCAGAAGAACTTGCCCAGCTGGAAGGCATCCTTAAGGAAGCTGAGGCCGCTTCGGAAAATGAAGAAATTGATATCTCCAAAGCGGCCCAGACCACGATAGAAACTGCCATTCACTCTCTGATCGAAACCTTGAAGAACAAGGAATTTGTATCAGCTGTGGCACAAGTCAAAGCTTTCAGGGTACAAGTTCAGTATTAA
Peptide sequence:
MPEPLAPESYPESICEEDVTLALKELDERCEEEEADFSGLSSQEEDEQDGFPEVQTSPLPTPFLSAIIAAFQPMAYEDEEEAWRCHVNQTLSDTDGSCAVFTFHVFSRLFQTIQRKFGEITKEAVGFLGESLQRIGTKFKSSLEVMMACSECPTVFVDAETLMACGLLETLKFGVLELQEHLDTYTTKREAAEQWLDNCKRTFGANEDIYRMNTNAHELELCRRLYRLHFQLLLLFQAYCKLINQVNTIKNEAEVINMSEELAQLEGILKEAEAASENEEIDISKAAQTTIETAIHSLIETLKNKEFVSAVAQVKAFRVQVQY
IRESfinder result
IRESfinder score
0.343237595
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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