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mmu_circFry_699
Basic information of circular RNA
PanCircBase ID:
mmu_circFry_699
mm39_Chr. Position ID:
chr5|150324257|150328886|+
Gene name:
Fry
Transcript:
ENSMUST00000087204.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
27,28,29,30,31
Exon/intron offsets:
0,807,2649,4150,4457
Genomic length:
4629
Exon/intron sizes in nt:
16,11,11,15,51,00,172
Flanking exon/introns:
chr5:150323169-150324257|chr5:150328886-150336648
Splice length:
699
Other database IDs:
CIRCpedia: MMU_CIRCpedia_220418, circAtlas: mmu-Fry_0013
Human Ortholog IDs:
Mature spliced Sequence:
TTCATCACAGAAGATTTCTCTTCCCCCAGCAAAGCCTACGGCACCATCTCTTCATCTTGTTCAGCCAGTGGGCTGGCCCCTTCAGCATCATGTTTACACCTCTGGATCGGTACAGTGACCGAAACCACCAGATAACGAGATACCAGTACTGTGCTCTAAAGGCAATGTCGGCAGTGCTGTGCTGTGGCCCTGTATTTGACAATGTCGGCCTCTCTCCGGACGGCTATCTGTATAAGTGGCTCGACAATATTCTGGCTTGTCAAGATTTACGAGTTCATCAGCTTGGCTGTGAGGTCGTCATGTTGCTACTGGAACTTAATCCTGACCAGATAAACCTTTTCAATTGGGCCATCGACCGATGTTACACAGGATCCTACCAGCTTGCTTCCGGCTGCTTCAAAGCCATAGCGACTGTGTGCGGAAACAGGAACTATCCCTTCGACATAGTGACACTGCTGAACCTTGTTCTCTTCAAGGCCTCTGACACCAACAGAGAGATTTACGAAGTCTCCATGCAGCTCATGCAGATCCTCGAAGCAAAGCTTTTTGTGCACTCCAAAAAGGTTGCGGAACAAAGACCTGGCAGTATTCTATACGGAACACATGGACCGCTGCCACCTCTCTACAGTGTGTCGCTAGCCCTTCTGTCGTGTGAGCTGGCCCGGATGTACCCCGAGCTCACTCTTCCCCTCTTCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTACAGTGTGTCGCTAGCCC
60.18
AACATGATGCTGAAGGGGCC
60.69
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGTCGCTAGCCCTTCTGTC
60.46
AACATGATGCTGAAGGGGCC
60.69
164
PCR template sequence:
ACACATGGACCGCTGCCACCTCTCTACAGTGTGTCGCTAGCCCTTCTGTCGTGTGAGCTGGCCCGGATGTACCCCGAGCTCACTCTTCCCCTCTTCTCAGTTCATCACAGAAGATTTCTCTTCCCCCAGCAAAGCCTACGGCACCATCTCTTCATCTTGTTCAGCCAGTGGGCTGGCCCCTTCAGCATCATGTTTACACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-30e-5p(1), mmu-miR-200c-3p(1), mmu-miR-101a-3p(1), mmu-miR-30a-5p(1), mmu-miR-1198-5p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCCTCTTCTCAGTTCATCACAGAAG
siRNA sets
TGATGAACTGAGAAGAGGG, TGTGATGAACTGAGAAGAG, GTGATGAACTGAGAAGAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRY_699_ORF_1
1.0333
0.211041146
0.99903998
1308
ORF sequence:
ATGTTTACACCTCTGGATCGGTACAGTGACCGAAACCACCAGATAACGAGATACCAGTACTGTGCTCTAAAGGCAATGTCGGCAGTGCTGTGCTGTGGCCCTGTATTTGACAATGTCGGCCTCTCTCCGGACGGCTATCTGTATAAGTGGCTCGACAATATTCTGGCTTGTCAAGATTTACGAGTTCATCAGCTTGGCTGTGAGGTCGTCATGTTGCTACTGGAACTTAATCCTGACCAGATAAACCTTTTCAATTGGGCCATCGACCGATGTTACACAGGATCCTACCAGCTTGCTTCCGGCTGCTTCAAAGCCATAGCGACTGTGTGCGGAAACAGGAACTATCCCTTCGACATAGTGACACTGCTGAACCTTGTTCTCTTCAAGGCCTCTGACACCAACAGAGAGATTTACGAAGTCTCCATGCAGCTCATGCAGATCCTCGAAGCAAAGCTTTTTGTGCACTCCAAAAAGGTTGCGGAACAAAGACCTGGCAGTATTCTATACGGAACACATGGACCGCTGCCACCTCTCTACAGTGTGTCGCTAGCCCTTCTGTCGTGTGAGCTGGCCCGGATGTACCCCGAGCTCACTCTTCCCCTCTTCTCAGTTCATCACAGAAGATTTCTCTTCCCCCAGCAAAGCCTACGGCACCATCTCTTCATCTTGTTCAGCCAGTGGGCTGGCCCCTTCAGCATCATGTTTACACCTCTGGATCGGTACAGTGACCGAAACCACCAGATAACGAGATACCAGTACTGTGCTCTAAAGGCAATGTCGGCAGTGCTGTGCTGTGGCCCTGTATTTGACAATGTCGGCCTCTCTCCGGACGGCTATCTGTATAAGTGGCTCGACAATATTCTGGCTTGTCAAGATTTACGAGTTCATCAGCTTGGCTGTGAGGTCGTCATGTTGCTACTGGAACTTAATCCTGACCAGATAAACCTTTTCAATTGGGCCATCGACCGATGTTACACAGGATCCTACCAGCTTGCTTCCGGCTGCTTCAAAGCCATAGCGACTGTGTGCGGAAACAGGAACTATCCCTTCGACATAGTGACACTGCTGAACCTTGTTCTCTTCAAGGCCTCTGACACCAACAGAGAGATTTACGAAGTCTCCATGCAGCTCATGCAGATCCTCGAAGCAAAGCTTTTTGTGCACTCCAAAAAGGTTGCGGAACAAAGACCTGGCAGTATTCTATACGGAACACATGGACCGCTGCCACCTCTCTACAGTGTGTCGCTAGCCCTTCTGTCGTGTGAGCTGGCCCGGATGTACCCCGAGCTCACTCTTCCCCTCTTCTCA
Peptide sequence:
MFTPLDRYSDRNHQITRYQYCALKAMSAVLCCGPVFDNVGLSPDGYLYKWLDNILACQDLRVHQLGCEVVMLLLELNPDQINLFNWAIDRCYTGSYQLASGCFKAIATVCGNRNYPFDIVTLLNLVLFKASDTNREIYEVSMQLMQILEAKLFVHSKKVAEQRPGSILYGTHGPLPPLYSVSLALLSCELARMYPELTLPLFSVHHRRFLFPQQSLRHHLFILFSQWAGPFSIMFTPLDRYSDRNHQITRYQYCALKAMSAVLCCGPVFDNVGLSPDGYLYKWLDNILACQDLRVHQLGCEVVMLLLELNPDQINLFNWAIDRCYTGSYQLASGCFKAIATVCGNRNYPFDIVTLLNLVLFKASDTNREIYEVSMQLMQILEAKLFVHSKKVAEQRPGSILYGTHGPLPPLYSVSLALLSCELARMYPELTLPLFS
IRESfinder result
IRESfinder score
0.576006892
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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