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mmu_circFry_690
Basic information of circular RNA
PanCircBase ID:
mmu_circFry_690
mm39_Chr. Position ID:
chr5|150393141|150405041|+
Gene name:
Fry
Transcript:
ENSMUST00000087204.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
54,55,56,57,58
Exon/intron offsets:
0,1744,8249,9096,11816
Genomic length:
11900
Exon/intron sizes in nt:
167,190,150,99,84
Flanking exon/introns:
chr5:150390411-150393141|chr5:150405041-150414542
Splice length:
690
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTGAGGCAGTCCCCGAAGAGGAGGACACCACCGTGCACGAAGATGATCTCTCAAGCTCCATCAATGAGCTCCCAGCAGCGTTTGAATGCAGCGACAGCTTCAGCCTGGATATGACGGAGGCGGAGGAGAAAGGAAATCGGGGCCTGGACCAGTACACTCTCGCCAGCTTCGGAGAAGGTGACAGGGGTGTGTCTCCCCCTCCCTCGCCCTTCTTCTCGGCCATCCTTGCTGCCTTTCAGCCCGCCGCCTGTGATGACGCCGAGGAAGCCTGGCGAAGCCACATTAACCAGCTCATGTGTGACTCAGACGGCTCCTGTGCTGTGTACACATTTCATGTGTTCTCCTCTCTGTTTAAGAATATTCAAAAGAGGTTCTGCTTCCTAACCTGTGATGCAGCCAGTTACCTTGGAGATAACCTCCGGGGAATCGGGTCCAAATTTGTCAGCTCTTCCCAGATGCTCACCTCCTGTTCTGAATGCCCTACGCTGTTCGTAGATGCAGAGACTCTCCTTTCCTGTGGGCTCCTGGACAAGCTCAAGTTCAGTGTGCTGGAACTGCAAGAATATCTGGACACGTACAACAACAGGAAAGAGGCCACACTCTCGTGGCTTGCCAACTGTAAGGCAACGTTTGCAGGTGGCTCGAGAGATGGAGTGATTACCTGCCAACCCGGGGACTCGGAAGAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCTCGAGAGATGGAGTGA
60.03
CATTCAAACGCTGCTGGGAG
59.83
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGCTCGAGAGATGGAGTG
59.9
CATTCAAACGCTGCTGGGAG
59.83
142
PCR template sequence:
AGAGGCCACACTCTCGTGGCTTGCCAACTGTAAGGCAACGTTTGCAGGTGGCTCGAGAGATGGAGTGATTACCTGCCAACCCGGGGACTCGGAAGAAAAGGCTGAGGCAGTCCCCGAAGAGGAGGACACCACCGTGCACGAAGATGATCTCTCAAGCTCCATCAATGAGCTCCCAGCAGCGTTTGAATGCAGCGACAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-181a-5p(1), mmu-miR-203-3p(1), mmu-miR-339-5p(1), mmu-miR-511-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCGGAAGAAAAGGCTGAGGCAGTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRY_690_ORF_1
0.981
0.308885254
0.999060622
1269
ORF sequence:
ATGACGGAGGCGGAGGAGAAAGGAAATCGGGGCCTGGACCAGTACACTCTCGCCAGCTTCGGAGAAGGTGACAGGGGTGTGTCTCCCCCTCCCTCGCCCTTCTTCTCGGCCATCCTTGCTGCCTTTCAGCCCGCCGCCTGTGATGACGCCGAGGAAGCCTGGCGAAGCCACATTAACCAGCTCATGTGTGACTCAGACGGCTCCTGTGCTGTGTACACATTTCATGTGTTCTCCTCTCTGTTTAAGAATATTCAAAAGAGGTTCTGCTTCCTAACCTGTGATGCAGCCAGTTACCTTGGAGATAACCTCCGGGGAATCGGGTCCAAATTTGTCAGCTCTTCCCAGATGCTCACCTCCTGTTCTGAATGCCCTACGCTGTTCGTAGATGCAGAGACTCTCCTTTCCTGTGGGCTCCTGGACAAGCTCAAGTTCAGTGTGCTGGAACTGCAAGAATATCTGGACACGTACAACAACAGGAAAGAGGCCACACTCTCGTGGCTTGCCAACTGTAAGGCAACGTTTGCAGGTGGCTCGAGAGATGGAGTGATTACCTGCCAACCCGGGGACTCGGAAGAAAAGGCTGAGGCAGTCCCCGAAGAGGAGGACACCACCGTGCACGAAGATGATCTCTCAAGCTCCATCAATGAGCTCCCAGCAGCGTTTGAATGCAGCGACAGCTTCAGCCTGGATATGACGGAGGCGGAGGAGAAAGGAAATCGGGGCCTGGACCAGTACACTCTCGCCAGCTTCGGAGAAGGTGACAGGGGTGTGTCTCCCCCTCCCTCGCCCTTCTTCTCGGCCATCCTTGCTGCCTTTCAGCCCGCCGCCTGTGATGACGCCGAGGAAGCCTGGCGAAGCCACATTAACCAGCTCATGTGTGACTCAGACGGCTCCTGTGCTGTGTACACATTTCATGTGTTCTCCTCTCTGTTTAAGAATATTCAAAAGAGGTTCTGCTTCCTAACCTGTGATGCAGCCAGTTACCTTGGAGATAACCTCCGGGGAATCGGGTCCAAATTTGTCAGCTCTTCCCAGATGCTCACCTCCTGTTCTGAATGCCCTACGCTGTTCGTAGATGCAGAGACTCTCCTTTCCTGTGGGCTCCTGGACAAGCTCAAGTTCAGTGTGCTGGAACTGCAAGAATATCTGGACACGTACAACAACAGGAAAGAGGCCACACTCTCGTGGCTTGCCAACTGTAAGGCAACGTTTGCAGGTGGCTCGAGAGATGGAGTGATTACCTGCCAACCCGGGGACTCGGAAGAAAAG
Peptide sequence:
MTEAEEKGNRGLDQYTLASFGEGDRGVSPPPSPFFSAILAAFQPAACDDAEEAWRSHINQLMCDSDGSCAVYTFHVFSSLFKNIQKRFCFLTCDAASYLGDNLRGIGSKFVSSSQMLTSCSECPTLFVDAETLLSCGLLDKLKFSVLELQEYLDTYNNRKEATLSWLANCKATFAGGSRDGVITCQPGDSEEKAEAVPEEEDTTVHEDDLSSSINELPAAFECSDSFSLDMTEAEEKGNRGLDQYTLASFGEGDRGVSPPPSPFFSAILAAFQPAACDDAEEAWRSHINQLMCDSDGSCAVYTFHVFSSLFKNIQKRFCFLTCDAASYLGDNLRGIGSKFVSSSQMLTSCSECPTLFVDAETLLSCGLLDKLKFSVLELQEYLDTYNNRKEATLSWLANCKATFAGGSRDGVITCQPGDSEEK
IRESfinder result
IRESfinder score
0.487199006
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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