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mmu_circFry_654
Basic information of circular RNA
PanCircBase ID:
mmu_circFry_654
mm39_Chr. Position ID:
chr5|150362950|150374685|+
Gene name:
Fry
Transcript:
ENSMUST00000087204.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
46,47,48,49
Exon/intron offsets:
0,6288,9934,11573
Genomic length:
11735
Exon/intron sizes in nt:
1,71,14,11,80,162
Flanking exon/introns:
chr5:150362273-150362950|chr5:150374685-150377964
Splice length:
654
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGCTTGGAAGAAAAAAACCCTAAACTTTCCAACCTGGCTCACGTCATGACCCTTTACAAAACACACAGCTACACAAGGGACTGCGCCACATGGGTCAACGTGGTCTGTCGCTACCTTCATGAAGCCTATGCCGACATCACCTTGAACATGGTCACCTACCTGGCAGAGTTGCTGGAGAAAGGCCTCCCCAGCATGCAGCAGCCGCTCCTGCAGGTCATCTACAGTCTACTCAGCTACATGGACCTCTCTGTCGTTCCTGTCAAGCAGTTTAACATGGAAGTCCTGAAGACCATTGAAAAATACGTACAGAGCATTCACTGGAGGGAAGCGTTGAATATCTTGAAGCTGGTTGTCTCTCGATCGGCCAGCCTGGTATTGCCTTCCTACCAACACAGCGACCTCTCCAAGATAGAACTACACCGGGTGTGGACCAGCGCCTCAAAGGAACTGCCGGGCAAGACCCTGGACTTCCACTTCGACATCTCAGAGACTCCAATCATTGGGAGACGGTACGATGAACTCCAGAATTCCTCTGGACGGGATGGGAAGCCTAGGGCCATGGCCGTCACCCGGAGTGCATCCTCTACCTCCTCCGGCTCCAACTCCAATGTCCTTGTTCCTGTGAGCTGGAAAAGACCCCAGTATTCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCCAACTCCAATGTCCT
59.67
GCAGTCCCTTGTGTAGCTGT
59.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCATCCTCTACCTCCTCCG
60.47
GCAGTCCCTTGTGTAGCTGT
59.96
163
PCR template sequence:
TAGGGCCATGGCCGTCACCCGGAGTGCATCCTCTACCTCCTCCGGCTCCAACTCCAATGTCCTTGTTCCTGTGAGCTGGAAAAGACCCCAGTATTCTCAGGTTTGCTTGGAAGAAAAAAACCCTAAACTTTCCAACCTGGCTCACGTCATGACCCTTTACAAAACACACAGCTACACAAGGGACTGCGCCACATGGGTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-328-3p(1), mmu-let-7d-5p(1), mmu-miR-431-5p(1), mmu-miR-541-5p(1), mmu-miR-98-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAGTATTCTCAGGTTTGCTTGGAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRY_654_ORF_1
1.2372
0.337066063
0.999445987
1260
ORF sequence:
ATGACCCTTTACAAAACACACAGCTACACAAGGGACTGCGCCACATGGGTCAACGTGGTCTGTCGCTACCTTCATGAAGCCTATGCCGACATCACCTTGAACATGGTCACCTACCTGGCAGAGTTGCTGGAGAAAGGCCTCCCCAGCATGCAGCAGCCGCTCCTGCAGGTCATCTACAGTCTACTCAGCTACATGGACCTCTCTGTCGTTCCTGTCAAGCAGTTTAACATGGAAGTCCTGAAGACCATTGAAAAATACGTACAGAGCATTCACTGGAGGGAAGCGTTGAATATCTTGAAGCTGGTTGTCTCTCGATCGGCCAGCCTGGTATTGCCTTCCTACCAACACAGCGACCTCTCCAAGATAGAACTACACCGGGTGTGGACCAGCGCCTCAAAGGAACTGCCGGGCAAGACCCTGGACTTCCACTTCGACATCTCAGAGACTCCAATCATTGGGAGACGGTACGATGAACTCCAGAATTCCTCTGGACGGGATGGGAAGCCTAGGGCCATGGCCGTCACCCGGAGTGCATCCTCTACCTCCTCCGGCTCCAACTCCAATGTCCTTGTTCCTGTGAGCTGGAAAAGACCCCAGTATTCTCAGGTTTGCTTGGAAGAAAAAAACCCTAAACTTTCCAACCTGGCTCACGTCATGACCCTTTACAAAACACACAGCTACACAAGGGACTGCGCCACATGGGTCAACGTGGTCTGTCGCTACCTTCATGAAGCCTATGCCGACATCACCTTGAACATGGTCACCTACCTGGCAGAGTTGCTGGAGAAAGGCCTCCCCAGCATGCAGCAGCCGCTCCTGCAGGTCATCTACAGTCTACTCAGCTACATGGACCTCTCTGTCGTTCCTGTCAAGCAGTTTAACATGGAAGTCCTGAAGACCATTGAAAAATACGTACAGAGCATTCACTGGAGGGAAGCGTTGAATATCTTGAAGCTGGTTGTCTCTCGATCGGCCAGCCTGGTATTGCCTTCCTACCAACACAGCGACCTCTCCAAGATAGAACTACACCGGGTGTGGACCAGCGCCTCAAAGGAACTGCCGGGCAAGACCCTGGACTTCCACTTCGACATCTCAGAGACTCCAATCATTGGGAGACGGTACGATGAACTCCAGAATTCCTCTGGACGGGATGGGAAGCCTAGGGCCATGGCCGTCACCCGGAGTGCATCCTCTACCTCCTCCGGCTCCAACTCCAATGTCCTTGTTCCTGTGAGCTGGAAAAGACCCCAGTATTCTCAG
Peptide sequence:
MTLYKTHSYTRDCATWVNVVCRYLHEAYADITLNMVTYLAELLEKGLPSMQQPLLQVIYSLLSYMDLSVVPVKQFNMEVLKTIEKYVQSIHWREALNILKLVVSRSASLVLPSYQHSDLSKIELHRVWTSASKELPGKTLDFHFDISETPIIGRRYDELQNSSGRDGKPRAMAVTRSASSTSSGSNSNVLVPVSWKRPQYSQVCLEEKNPKLSNLAHVMTLYKTHSYTRDCATWVNVVCRYLHEAYADITLNMVTYLAELLEKGLPSMQQPLLQVIYSLLSYMDLSVVPVKQFNMEVLKTIEKYVQSIHWREALNILKLVVSRSASLVLPSYQHSDLSKIELHRVWTSASKELPGKTLDFHFDISETPIIGRRYDELQNSSGRDGKPRAMAVTRSASSTSSGSNSNVLVPVSWKRPQYSQ
IRESfinder result
IRESfinder score
0.558721708
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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