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mmu_circFrrs1_449
Basic information of circular RNA
PanCircBase ID:
mmu_circFrrs1_449
mm39_Chr. Position ID:
chr3|116671882|116675501|+
Gene name:
Frrs1
Transcript:
ENSMUST00000040260.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,2533,3524
Genomic length:
3619
Exon/intron sizes in nt:
217,137,95
Flanking exon/introns:
chr3:116659926-116671882|chr3:116675501-116676697
Splice length:
449
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGACGTGTCCTCACTTGGGAATGGCGGCTCCCCAGATCACCCTCAGTGTCCTTGTGATTGCCTTGCTCACTTGCTCTGTGACTGCTTATCCCAATGGCAAAGTACCAATGTCCTGTGGTGGGATGATCCCCCAACATAACCACAGCCCACAGTCTGAGCCTATTCACCAAATCACAGTGAGTCAGACGACGTTCAAACCTGGAGACCAGATCAAAGTTACTTTATCAGGACCACCATTTAGAGGCTTTCTTTTGGAAGCTCGTGATGCTGAGAATTTGAGCGGCCCTCCTATTGGCTCCTTCACTCTGATTGACAGCGAAGAGTCACAACTTCTGACCTGTACCGATGTACAGGGATTAGCTGTGAGTCATACAAGGTCATCCAAGAAGACAGAAATTAAAGTCTACTGGGATGCTCCAAGCCCTGCCCCAGATCACATACGATTTCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTACTGGGATGCTCCAAGCC
60.18
TGCCATTGGGATAAGCAGTCA
59.72
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTACTGGGATGCTCCAAGCC
60.18
GCCATTGGGATAAGCAGTCAC
59.32
144
PCR template sequence:
TACAGGGATTAGCTGTGAGTCATACAAGGTCATCCAAGAAGACAGAAATTAAAGTCTACTGGGATGCTCCAAGCCCTGCCCCAGATCACATACGATTTCTGTGACGTGTCCTCACTTGGGAATGGCGGCTCCCCAGATCACCCTCAGTGTCCTTGTGATTGCCTTGCTCACTTGCTCTGTGACTGCTTATCCCAATGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(1), mmu-miR-673-5p(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACATACGATTTCTGTGACGTGTCCTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRRS1_449_ORF_1
0.8905
0.123966934
0.504049714
432
ORF sequence:
ATGGCGGCTCCCCAGATCACCCTCAGTGTCCTTGTGATTGCCTTGCTCACTTGCTCTGTGACTGCTTATCCCAATGGCAAAGTACCAATGTCCTGTGGTGGGATGATCCCCCAACATAACCACAGCCCACAGTCTGAGCCTATTCACCAAATCACAGTGAGTCAGACGACGTTCAAACCTGGAGACCAGATCAAAGTTACTTTATCAGGACCACCATTTAGAGGCTTTCTTTTGGAAGCTCGTGATGCTGAGAATTTGAGCGGCCCTCCTATTGGCTCCTTCACTCTGATTGACAGCGAAGAGTCACAACTTCTGACCTGTACCGATGTACAGGGATTAGCTGTGAGTCATACAAGGTCATCCAAGAAGACAGAAATTAAAGTCTACTGGGATGCTCCAAGCCCTGCCCCAGATCACATACGATTTCTGTGA
Peptide sequence:
MAAPQITLSVLVIALLTCSVTAYPNGKVPMSCGGMIPQHNHSPQSEPIHQITVSQTTFKPGDQIKVTLSGPPFRGFLLEARDAENLSGPPIGSFTLIDSEESQLLTCTDVQGLAVSHTRSSKKTEIKVYWDAPSPAPDHIRFL
IRESfinder result
IRESfinder score
0.70935364
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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