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mmu_circFrmd5_105
Basic information of circular RNA
PanCircBase ID:
mmu_circFrmd5_105
mm39_Chr. Position ID:
chr2|121422138|121422243|-
Gene name:
Frmd5
Transcript:
ENSMUST00000110593.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
105
Exon/intron sizes in nt:
105
Flanking exon/introns:
chr2:121416952-121422138|chr2:121422243-121595511
Splice length:
105
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGAGATGCCAAAGGCCAGTACCTGTTTGACCTCCTTTGCCACCACCTGAACCTACTTGAGAAAGACTACTTTGGGATTCGTTTTGTGGACCCAGATAAGCAGCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGATAAGCAGCGGAGAG
59.97
TTCAGGTGGTGGCAAAGGAG
60.18
66
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGATAAGCAGCGGAGAG
59.97
TGGTGGCAAAGGAGGTCAAA
59.74
60
PCR template sequence:
ACTTGAGAAAGACTACTTTGGGATTCGTTTTGTGGACCCAGATAAGCAGCGGAGAGATGCCAAAGGCCAGTACCTGTTTGACCTCCTTTGCCACCACCTGAACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-26a-5p(1), mmu-miR-26b-5p(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGATAAGCAGCGGAGAGATGCCAAAG
siRNA sets
TTTGGCATCTCTCCGCTGC, TTGGCATCTCTCCGCTGCT, CTTTGGCATCTCTCCGCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.629517714
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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