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mmu_circFras1_2298
Basic information of circular RNA
PanCircBase ID:
mmu_circFras1_2298
mm39_Chr. Position ID:
chr5|96783916|96834602|+
Gene name:
Fras1
Transcript:
ENSMUST00000036019.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
21,22,23,24,25,26,27,28,29,30,31,32,33,34,35
Exon/intron offsets:
0,674,6430,9020,9770,11440,14363,18182,21088,31340,37454,41209,46226,48298,50653
Genomic length:
50686
Exon/intron sizes in nt:
153,147,147,141,141,141,271,85,327,154,188,117,115,138,33
Flanking exon/introns:
chr5:96766361-96783916|chr5:96834602-96839199
Splice length:
2298
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACTGCCATCCTTTGTGCCAGCACTGTGTGGCCAATCTCCAAGACACTGGTAGTATCTGCCTAAAGTGTCAGCATGCCCGCCACCTGCTGCTTGGAGATCACTGTGTGCCAGAATGCCCTCCTGGACACTATAAAGAGAGAGGCACTTGTAAAACATGCCATTCCTCCTGCAGAAGCTGCCAGAATGGAGGACCTTTCTCCTGCTCCTCTTGTGACACTGGTCTGGTTCTGACCCACATTGGTACCTGTAGCACCGCCTGCTTCCCTGGGCATTACCTGGATGATAACCAAGTTTGCCAGCCATGCAACAGGCATTGCAGAAGCTGTGATTCACAGGGCAGCTGCACCTCCTGCCGAGATCCAAGCAAGGTCCTGCTCTTTGGGGAATGCCAGTATGAGAGCTGCACCCCCCAGTACTACCTTGACATCGCCACGAAGACTTGCAAAGAGTGTGACTGGAGTTGCAACGCCTGTACTGGGCCTCTTCGAACAGACTGTCTGCAGTGCATGGACGGCTATGTTCTCCAGGATGGTGTGTGTGTGGAGCAGTGCTCACCACAACACTACCGGGACTCCGGCTCCTGCAAGCGCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGGGAAAGGATACTTTGCAGATCATGCCAAGCACAGATGCATAGCCTGCCCTCAGGGATGCTTGCGGTGCAGCCACAAAGATCGGTGTCACTTATGCGACCACAGTTTCTTTCTGAAGAGTGGTCTCTGCATGCCCACCTGTGTCCCTGGTTTCTCTGGCCACTCATCTAATGAAAACTGTACTGACAAAATGTACACTCCAAGTCTTCATGTGAACGGCTCCTTGACCCTTGGAATTGGTTCAATGAAGCCACTGGACTTTTCTCTCCTGAACATCCAACACCAGGATGGCAGAGTGGAAGATCTGCTGTTCCACGTTGTAAGCACACCCACCAACGGTCAGCTGCTGCTCTCAAGAAATGGCAAGGAGGTCCAGCTGGAGAAGGCTGGCCATTTTAGCTGGAAAGATGTGAATGAGAAGAAAGTGCGCTTTGTACACAGCAAAGAGAAGCTCAGGAAAGGCTACTTTTCCTTGAAAATTAGTGACCAGCAATTCTTCTCTGAGCCACAGCTGATCAACATACAGGCCTTTTCAACGCAGGCTCCGTATGTGCTGAGAAATGAAGTTCTCCACGTCAGCAAAGGAGAGCGGGCAACCATCACCACCCAGCTGCTTGACATCCGGGATGATGACAACCCGCAGGATGTGGTCGTGAATGTGCTTGATCCTCCTCTTCATGGCCAATTGCTCCAAATGCCTCCAGCCCCTGCAGCCTCTATTTATCAGTTCCATCTGGATGAGCTCTCCAGGGGCCTCCTCCTCTATGCTCACGATGGCTCAGACAGCACATCTGATATTATAGTCTTTCAGGCCAATGATGGACACTCCTTCCAAAATATACTGTTTCACGTGAAGAACATACCCAAGAATGACAGAGCTCTGCGACTTGTGACCAACTCTATGGTGTGGGTTCCGGAAGGGGGAATGCTGAAGATCACCAACCGAATCTTGAAGGCCCAAGCTCCAGGTGTCAGAGCCGACGACATCATCTACAAGATTACACACAGCCGCCCCCAGTTTGGGGAGGTGGTCCTCCTGATGAATCTGCCTGCAGACAGTCCAGCCGGTCCTGCGGAGGAAGGGCATCACCTGCCTGATGGAAGGATGGCAACCCCCATCAGTACCTTTACCCAGCAGGACATCGATGATGGCGTTGTGTGGTACAGACACTTGGGAGCCCCCACGCAGAGTGACTCCTTCCGCTTTCAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCTCCAGGAGATCATGGC
59.89
TGATCTCCAAGCAGCAGGTG
60.04
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCTTCTCATTTGCTGGT
59.89
TGATCTCCAAGCAGCAGGTG
60.04
151
PCR template sequence:
CAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGACTGCCATCCTTTGTGCCAGCACTGTGTGGCCAATCTCCAAGACACTGGTAGTATCTGCCTAAAGTGTCAGCATGCCCGCCACCTGCTGCTTGGAGATCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
28
Interacting miRNAs (#binding sites)
mmu-miR-673-5p(3), mmu-miR-122-5p(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-150-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-324-5p(1), mmu-miR-185-5p(1), mmu-miR-181b-5p(1), mmu-miR-433-5p(1), mmu-miR-351-5p(1), mmu-miR-382-3p(1), mmu-miR-22-3p(1), mmu-miR-370-3p(1), mmu-miR-342-5p(1), mmu-miR-499-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1), mmu-miR-3470b(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTTACTTCACAGACTGCCATCCTTT
siRNA sets
AAAGGATGGCAGTCTGTGA, AAGGATGGCAGTCTGTGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRAS1_2298_ORF_1
1.0294
0.2228378
1
4089
ORF sequence:
ATGGACGGCTATGTTCTCCAGGATGGTGTGTGTGTGGAGCAGTGCTCACCACAACACTACCGGGACTCCGGCTCCTGCAAGCGCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGGGAAAGGATACTTTGCAGATCATGCCAAGCACAGATGCATAGCCTGCCCTCAGGGATGCTTGCGGTGCAGCCACAAAGATCGGTGTCACTTATGCGACCACAGTTTCTTTCTGAAGAGTGGTCTCTGCATGCCCACCTGTGTCCCTGGTTTCTCTGGCCACTCATCTAATGAAAACTGTACTGACAAAATGTACACTCCAAGTCTTCATGTGAACGGCTCCTTGACCCTTGGAATTGGTTCAATGAAGCCACTGGACTTTTCTCTCCTGAACATCCAACACCAGGATGGCAGAGTGGAAGATCTGCTGTTCCACGTTGTAAGCACACCCACCAACGGTCAGCTGCTGCTCTCAAGAAATGGCAAGGAGGTCCAGCTGGAGAAGGCTGGCCATTTTAGCTGGAAAGATGTGAATGAGAAGAAAGTGCGCTTTGTACACAGCAAAGAGAAGCTCAGGAAAGGCTACTTTTCCTTGAAAATTAGTGACCAGCAATTCTTCTCTGAGCCACAGCTGATCAACATACAGGCCTTTTCAACGCAGGCTCCGTATGTGCTGAGAAATGAAGTTCTCCACGTCAGCAAAGGAGAGCGGGCAACCATCACCACCCAGCTGCTTGACATCCGGGATGATGACAACCCGCAGGATGTGGTCGTGAATGTGCTTGATCCTCCTCTTCATGGCCAATTGCTCCAAATGCCTCCAGCCCCTGCAGCCTCTATTTATCAGTTCCATCTGGATGAGCTCTCCAGGGGCCTCCTCCTCTATGCTCACGATGGCTCAGACAGCACATCTGATATTATAGTCTTTCAGGCCAATGATGGACACTCCTTCCAAAATATACTGTTTCACGTGAAGAACATACCCAAGAATGACAGAGCTCTGCGACTTGTGACCAACTCTATGGTGTGGGTTCCGGAAGGGGGAATGCTGAAGATCACCAACCGAATCTTGAAGGCCCAAGCTCCAGGTGTCAGAGCCGACGACATCATCTACAAGATTACACACAGCCGCCCCCAGTTTGGGGAGGTGGTCCTCCTGATGAATCTGCCTGCAGACAGTCCAGCCGGTCCTGCGGAGGAAGGGCATCACCTGCCTGATGGAAGGATGGCAACCCCCATCAGTACCTTTACCCAGCAGGACATCGATGATGGCGTTGTGTGGTACAGACACTTGGGAGCCCCCACGCAGAGTGACTCCTTCCGCTTTCAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGACTGCCATCCTTTGTGCCAGCACTGTGTGGCCAATCTCCAAGACACTGGTAGTATCTGCCTAAAGTGTCAGCATGCCCGCCACCTGCTGCTTGGAGATCACTGTGTGCCAGAATGCCCTCCTGGACACTATAAAGAGAGAGGCACTTGTAAAACATGCCATTCCTCCTGCAGAAGCTGCCAGAATGGAGGACCTTTCTCCTGCTCCTCTTGTGACACTGGTCTGGTTCTGACCCACATTGGTACCTGTAGCACCGCCTGCTTCCCTGGGCATTACCTGGATGATAACCAAGTTTGCCAGCCATGCAACAGGCATTGCAGAAGCTGTGATTCACAGGGCAGCTGCACCTCCTGCCGAGATCCAAGCAAGGTCCTGCTCTTTGGGGAATGCCAGTATGAGAGCTGCACCCCCCAGTACTACCTTGACATCGCCACGAAGACTTGCAAAGAGTGTGACTGGAGTTGCAACGCCTGTACTGGGCCTCTTCGAACAGACTGTCTGCAGTGCATGGACGGCTATGTTCTCCAGGATGGTGTGTGTGTGGAGCAGTGCTCACCACAACACTACCGGGACTCCGGCTCCTGCAAGCGCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGGGAAAGGATACTTTGCAGATCATGCCAAGCACAGATGCATAGCCTGCCCTCAGGGATGCTTGCGGTGCAGCCACAAAGATCGGTGTCACTTATGCGACCACAGTTTCTTTCTGAAGAGTGGTCTCTGCATGCCCACCTGTGTCCCTGGTTTCTCTGGCCACTCATCTAATGAAAACTGTACTGACAAAATGTACACTCCAAGTCTTCATGTGAACGGCTCCTTGACCCTTGGAATTGGTTCAATGAAGCCACTGGACTTTTCTCTCCTGAACATCCAACACCAGGATGGCAGAGTGGAAGATCTGCTGTTCCACGTTGTAAGCACACCCACCAACGGTCAGCTGCTGCTCTCAAGAAATGGCAAGGAGGTCCAGCTGGAGAAGGCTGGCCATTTTAGCTGGAAAGATGTGAATGAGAAGAAAGTGCGCTTTGTACACAGCAAAGAGAAGCTCAGGAAAGGCTACTTTTCCTTGAAAATTAGTGACCAGCAATTCTTCTCTGAGCCACAGCTGATCAACATACAGGCCTTTTCAACGCAGGCTCCGTATGTGCTGAGAAATGAAGTTCTCCACGTCAGCAAAGGAGAGCGGGCAACCATCACCACCCAGCTGCTTGACATCCGGGATGATGACAACCCGCAGGATGTGGTCGTGAATGTGCTTGATCCTCCTCTTCATGGCCAATTGCTCCAAATGCCTCCAGCCCCTGCAGCCTCTATTTATCAGTTCCATCTGGATGAGCTCTCCAGGGGCCTCCTCCTCTATGCTCACGATGGCTCAGACAGCACATCTGATATTATAGTCTTTCAGGCCAATGATGGACACTCCTTCCAAAATATACTGTTTCACGTGAAGAACATACCCAAGAATGACAGAGCTCTGCGACTTGTGACCAACTCTATGGTGTGGGTTCCGGAAGGGGGAATGCTGAAGATCACCAACCGAATCTTGAAGGCCCAAGCTCCAGGTGTCAGAGCCGACGACATCATCTACAAGATTACACACAGCCGCCCCCAGTTTGGGGAGGTGGTCCTCCTGATGAATCTGCCTGCAGACAGTCCAGCCGGTCCTGCGGAGGAAGGGCATCACCTGCCTGATGGAAGGATGGCAACCCCCATCAGTACCTTTACCCAGCAGGACATCGATGATGGCGTTGTGTGGTACAGACACTTGGGAGCCCCCACGCAGAGTGACTCCTTCCGCTTTCAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACA
Peptide sequence:
MDGYVLQDGVCVEQCSPQHYRDSGSCKRCDSHCVECQGPHECTRCEEPFLLFQAQCVQECGKGYFADHAKHRCIACPQGCLRCSHKDRCHLCDHSFFLKSGLCMPTCVPGFSGHSSNENCTDKMYTPSLHVNGSLTLGIGSMKPLDFSLLNIQHQDGRVEDLLFHVVSTPTNGQLLLSRNGKEVQLEKAGHFSWKDVNEKKVRFVHSKEKLRKGYFSLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHVSKGERATITTQLLDIRDDDNPQDVVVNVLDPPLHGQLLQMPPAPAASIYQFHLDELSRGLLLYAHDGSDSTSDIIVFQANDGHSFQNILFHVKNIPKNDRALRLVTNSMVWVPEGGMLKITNRILKAQAPGVRADDIIYKITHSRPQFGEVVLLMNLPADSPAGPAEEGHHLPDGRMATPISTFTQQDIDDGVVWYRHLGAPTQSDSFRFQVSSATSAQEHLESHMFNIAILPQAPEAPKLSLGTSLHMTAREDGLSVIQPQSLSFVKAESPSGKIIYNITVPLHPNQGIIEHRDRPHSPIQYFTQEDINQGQIMYRPPVAPPHLQEIMAFSFAGLPESVKFYFTDCHPLCQHCVANLQDTGSICLKCQHARHLLLGDHCVPECPPGHYKERGTCKTCHSSCRSCQNGGPFSCSSCDTGLVLTHIGTCSTACFPGHYLDDNQVCQPCNRHCRSCDSQGSCTSCRDPSKVLLFGECQYESCTPQYYLDIATKTCKECDWSCNACTGPLRTDCLQCMDGYVLQDGVCVEQCSPQHYRDSGSCKRCDSHCVECQGPHECTRCEEPFLLFQAQCVQECGKGYFADHAKHRCIACPQGCLRCSHKDRCHLCDHSFFLKSGLCMPTCVPGFSGHSSNENCTDKMYTPSLHVNGSLTLGIGSMKPLDFSLLNIQHQDGRVEDLLFHVVSTPTNGQLLLSRNGKEVQLEKAGHFSWKDVNEKKVRFVHSKEKLRKGYFSLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHVSKGERATITTQLLDIRDDDNPQDVVVNVLDPPLHGQLLQMPPAPAASIYQFHLDELSRGLLLYAHDGSDSTSDIIVFQANDGHSFQNILFHVKNIPKNDRALRLVTNSMVWVPEGGMLKITNRILKAQAPGVRADDIIYKITHSRPQFGEVVLLMNLPADSPAGPAEEGHHLPDGRMATPISTFTQQDIDDGVVWYRHLGAPTQSDSFRFQVSSATSAQEHLESHMFNIAILPQAPEAPKLSLGTSLHMTAREDGLSVIQPQSLSFVKAESPSGKIIYNITVPLHPNQGIIEHRDRPHSPIQYFTQEDINQGQIMYRPPVAPPHLQEIMAFSFAGLPESVKFYFT
IRESfinder result
IRESfinder score
0.452737889
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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