top of page
mmu_circFras1_2216
Basic information of circular RNA
PanCircBase ID:
mmu_circFras1_2216
mm39_Chr. Position ID:
chr5|96793686|96843006|+
Gene name:
Fras1
Transcript:
ENSMUST00000036019.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
25,26,27,28,29,30,31,32,33,34,35,36,37,38
Exon/intron offsets:
0,1670,4593,8412,11318,21570,27684,31439,36456,38528,40883,45513,46709,49072
Genomic length:
49320
Exon/intron sizes in nt:
1,41,14,12,71,85,32,71,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr5:96793077-96793686|chr5:96843006-96848014
Splice length:
2216
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGGGAAAGGATACTTTGCAGATCATGCCAAGCACAGATGCATAGCCTGCCCTCAGGGATGCTTGCGGTGCAGCCACAAAGATCGGTGTCACTTATGCGACCACAGTTTCTTTCTGAAGAGTGGTCTCTGCATGCCCACCTGTGTCCCTGGTTTCTCTGGCCACTCATCTAATGAAAACTGTACTGACAAAATGTACACTCCAAGTCTTCATGTGAACGGCTCCTTGACCCTTGGAATTGGTTCAATGAAGCCACTGGACTTTTCTCTCCTGAACATCCAACACCAGGATGGCAGAGTGGAAGATCTGCTGTTCCACGTTGTAAGCACACCCACCAACGGTCAGCTGCTGCTCTCAAGAAATGGCAAGGAGGTCCAGCTGGAGAAGGCTGGCCATTTTAGCTGGAAAGATGTGAATGAGAAGAAAGTGCGCTTTGTACACAGCAAAGAGAAGCTCAGGAAAGGCTACTTTTCCTTGAAAATTAGTGACCAGCAATTCTTCTCTGAGCCACAGCTGATCAACATACAGGCCTTTTCAACGCAGGCTCCGTATGTGCTGAGAAATGAAGTTCTCCACGTCAGCAAAGGAGAGCGGGCAACCATCACCACCCAGCTGCTTGACATCCGGGATGATGACAACCCGCAGGATGTGGTCGTGAATGTGCTTGATCCTCCTCTTCATGGCCAATTGCTCCAAATGCCTCCAGCCCCTGCAGCCTCTATTTATCAGTTCCATCTGGATGAGCTCTCCAGGGGCCTCCTCCTCTATGCTCACGATGGCTCAGACAGCACATCTGATATTATAGTCTTTCAGGCCAATGATGGACACTCCTTCCAAAATATACTGTTTCACGTGAAGAACATACCCAAGAATGACAGAGCTCTGCGACTTGTGACCAACTCTATGGTGTGGGTTCCGGAAGGGGGAATGCTGAAGATCACCAACCGAATCTTGAAGGCCCAAGCTCCAGGTGTCAGAGCCGACGACATCATCTACAAGATTACACACAGCCGCCCCCAGTTTGGGGAGGTGGTCCTCCTGATGAATCTGCCTGCAGACAGTCCAGCCGGTCCTGCGGAGGAAGGGCATCACCTGCCTGATGGAAGGATGGCAACCCCCATCAGTACCTTTACCCAGCAGGACATCGATGATGGCGTTGTGTGGTACAGACACTTGGGAGCCCCCACGCAGAGTGACTCCTTCCGCTTTCAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGTTTCTGATGGGCAGCACACGAGTCCAGAGATGGCCCTCACCATTCACTTGCTGCACTCTGATCTGCAACCACCAGCATTTCAGGTCAAGGCTCCCCTGCTGGAAGTTAGCCCTGGAGGCCGGACCTCTTTAGGCCTGCAGTTGTTAGTGAGAGATGCTCAGGTGGTGCCAGAAGAGCTCTTCTTCCAGCTTCAGAAGTCTCCTCAGCATGGCATGCTCGTGAAGTACACGGCAAAGTCCTCGGTGACCATGGCTGCAGGTGACACTTTTACATATGATGAAGTCGAGAGAAACGTTCTGCAGTACGTACATGATGGGTCCTCTGCGTGGGAGGACAGCCTGGAGATCTCAGTCACAGATGGCCTGACAGTGACAACATCAGAAGTAAAAGTGGAGGTGTCCCCATCAGAGAACCGAGGGCCCAGGCTGGCCCCCGGCTCCTCCCTGAGCATGACGGTTGCTAGTCAACACACGGCCATAATCACCAGGTCACACCTTGCCTATGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCCTGAGCATGACGGTTG
60.74
CACTGGGCTTGGAAGAGGAG
60.04
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCCTGAGCATGACGGTTG
60.74
GACACACTGGGCTTGGAAGA
59.89
156
PCR template sequence:
AGAGAACCGAGGGCCCAGGCTGGCCCCCGGCTCCTCCCTGAGCATGACGGTTGCTAGTCAACACACGGCCATAATCACCAGGTCACACCTTGCCTATGTGGCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
29
Interacting miRNAs (#binding sites)
mmu-miR-122-5p(2), mmu-miR-134-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-150-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-324-5p(1), mmu-miR-138-5p(1), mmu-miR-185-5p(1), mmu-miR-181b-5p(1), mmu-miR-434-5p(1), mmu-miR-433-5p(1), mmu-miR-351-5p(1), mmu-miR-382-3p(1), mmu-miR-127-5p(1), mmu-miR-370-3p(1), mmu-miR-499-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1), mmu-miR-3470b(1), mmu-miR-670-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTTGCCTATGTGGCTGTGACAGCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRAS1_2216_ORF_1
1.231
0.273188873
0.99999853
2130
ORF sequence:
ATGCCCACCTGTGTCCCTGGTTTCTCTGGCCACTCATCTAATGAAAACTGTACTGACAAAATGTACACTCCAAGTCTTCATGTGAACGGCTCCTTGACCCTTGGAATTGGTTCAATGAAGCCACTGGACTTTTCTCTCCTGAACATCCAACACCAGGATGGCAGAGTGGAAGATCTGCTGTTCCACGTTGTAAGCACACCCACCAACGGTCAGCTGCTGCTCTCAAGAAATGGCAAGGAGGTCCAGCTGGAGAAGGCTGGCCATTTTAGCTGGAAAGATGTGAATGAGAAGAAAGTGCGCTTTGTACACAGCAAAGAGAAGCTCAGGAAAGGCTACTTTTCCTTGAAAATTAGTGACCAGCAATTCTTCTCTGAGCCACAGCTGATCAACATACAGGCCTTTTCAACGCAGGCTCCGTATGTGCTGAGAAATGAAGTTCTCCACGTCAGCAAAGGAGAGCGGGCAACCATCACCACCCAGCTGCTTGACATCCGGGATGATGACAACCCGCAGGATGTGGTCGTGAATGTGCTTGATCCTCCTCTTCATGGCCAATTGCTCCAAATGCCTCCAGCCCCTGCAGCCTCTATTTATCAGTTCCATCTGGATGAGCTCTCCAGGGGCCTCCTCCTCTATGCTCACGATGGCTCAGACAGCACATCTGATATTATAGTCTTTCAGGCCAATGATGGACACTCCTTCCAAAATATACTGTTTCACGTGAAGAACATACCCAAGAATGACAGAGCTCTGCGACTTGTGACCAACTCTATGGTGTGGGTTCCGGAAGGGGGAATGCTGAAGATCACCAACCGAATCTTGAAGGCCCAAGCTCCAGGTGTCAGAGCCGACGACATCATCTACAAGATTACACACAGCCGCCCCCAGTTTGGGGAGGTGGTCCTCCTGATGAATCTGCCTGCAGACAGTCCAGCCGGTCCTGCGGAGGAAGGGCATCACCTGCCTGATGGAAGGATGGCAACCCCCATCAGTACCTTTACCCAGCAGGACATCGATGATGGCGTTGTGTGGTACAGACACTTGGGAGCCCCCACGCAGAGTGACTCCTTCCGCTTTCAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGTTTCTGATGGGCAGCACACGAGTCCAGAGATGGCCCTCACCATTCACTTGCTGCACTCTGATCTGCAACCACCAGCATTTCAGGTCAAGGCTCCCCTGCTGGAAGTTAGCCCTGGAGGCCGGACCTCTTTAGGCCTGCAGTTGTTAGTGAGAGATGCTCAGGTGGTGCCAGAAGAGCTCTTCTTCCAGCTTCAGAAGTCTCCTCAGCATGGCATGCTCGTGAAGTACACGGCAAAGTCCTCGGTGACCATGGCTGCAGGTGACACTTTTACATATGATGAAGTCGAGAGAAACGTTCTGCAGTACGTACATGATGGGTCCTCTGCGTGGGAGGACAGCCTGGAGATCTCAGTCACAGATGGCCTGACAGTGACAACATCAGAAGTAAAAGTGGAGGTGTCCCCATCAGAGAACCGAGGGCCCAGGCTGGCCCCCGGCTCCTCCCTGAGCATGACGGTTGCTAGTCAACACACGGCCATAATCACCAGGTCACACCTTGCCTATGTGGCTGTGACAGCCACTGTGTTGAGTGCCAGGGTCCCCATGAGTGCACACGCTGTGAAGAGCCCTTTCTCCTCTTCCAAGCCCAGTGTGTCCAAGAATGTGGGAAAGGATACTTTGCAGATCATGCCAAGCACAGATGCATAG
Peptide sequence:
MPTCVPGFSGHSSNENCTDKMYTPSLHVNGSLTLGIGSMKPLDFSLLNIQHQDGRVEDLLFHVVSTPTNGQLLLSRNGKEVQLEKAGHFSWKDVNEKKVRFVHSKEKLRKGYFSLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHVSKGERATITTQLLDIRDDDNPQDVVVNVLDPPLHGQLLQMPPAPAASIYQFHLDELSRGLLLYAHDGSDSTSDIIVFQANDGHSFQNILFHVKNIPKNDRALRLVTNSMVWVPEGGMLKITNRILKAQAPGVRADDIIYKITHSRPQFGEVVLLMNLPADSPAGPAEEGHHLPDGRMATPISTFTQQDIDDGVVWYRHLGAPTQSDSFRFQVSSATSAQEHLESHMFNIAILPQAPEAPKLSLGTSLHMTAREDGLSVIQPQSLSFVKAESPSGKIIYNITVPLHPNQGIIEHRDRPHSPIQYFTQEDINQGQIMYRPPVAPPHLQEIMAFSFAGLPESVKFYFTVSDGQHTSPEMALTIHLLHSDLQPPAFQVKAPLLEVSPGGRTSLGLQLLVRDAQVVPEELFFQLQKSPQHGMLVKYTAKSSVTMAAGDTFTYDEVERNVLQYVHDGSSAWEDSLEISVTDGLTVTTSEVKVEVSPSENRGPRLAPGSSLSMTVASQHTAIITRSHLAYVAVTATVLSARVPMSAHAVKSPFSSSKPSVSKNVGKDTLQIMPSTDA
IRESfinder result
IRESfinder score
0.435821829
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page