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mmu_circFras1_2155
Basic information of circular RNA
PanCircBase ID:
mmu_circFras1_2155
mm39_Chr. Position ID:
chr5|96825125|96860424|+
Gene name:
Fras1
Transcript:
ENSMUST00000036019.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
32,33,34,35,36,37,38,39,40,41,42,43,44,45
Exon/intron offsets:
0,5017,7089,9444,14074,15270,17633,22889,23233,24874,30038,31355,32598,35110
Genomic length:
35299
Exon/intron sizes in nt:
11,71,15,13,83,31,32,10,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr5:96821558-96825125|chr5:96860424-96861836
Splice length:
2155
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGTTTCTGATGGGCAGCACACGAGTCCAGAGATGGCCCTCACCATTCACTTGCTGCACTCTGATCTGCAACCACCAGCATTTCAGGTCAAGGCTCCCCTGCTGGAAGTTAGCCCTGGAGGCCGGACCTCTTTAGGCCTGCAGTTGTTAGTGAGAGATGCTCAGGTGGTGCCAGAAGAGCTCTTCTTCCAGCTTCAGAAGTCTCCTCAGCATGGCATGCTCGTGAAGTACACGGCAAAGTCCTCGGTGACCATGGCTGCAGGTGACACTTTTACATATGATGAAGTCGAGAGAAACGTTCTGCAGTACGTACATGATGGGTCCTCTGCGTGGGAGGACAGCCTGGAGATCTCAGTCACAGATGGCCTGACAGTGACAACATCAGAAGTAAAAGTGGAGGTGTCCCCATCAGAGAACCGAGGGCCCAGGCTGGCCCCCGGCTCCTCCCTGAGCATGACGGTTGCTAGTCAACACACGGCCATAATCACCAGGTCACACCTTGCCTATGTGGATGATTCTTCCTCTGACCCAGAGATCTGGATTCGGTTAAGTTCTCTGCCACTGTATGGCGTACTCTTCAGAAGCTCAGGGCCTGACATGGACGAGCTCTCGGGGGATTCCAACTTCACCATGGAGGACATCAACAAGAAGAACATTAGGTACTCAGCTGTGTTTGAGACGGACGGGCATTCGGTTACAGATGGCTTCCACTTCTCTGTCTCAGACATGGACGGCAACCATGTGGACAACCAGGTCTTTACCATCACGGTCACTCCTGCCGAGAACCCTCCACACATCATTGCCTTTGCTGACCTTATCACGGTTGATGAGGGAGGGAGAGCACCACTCTCTCTCCACCACTTCTTTGCGACTGAAGATCAGGACAACCTTCAGGACGACGCTGTCATTAAACTAAGCGCTCTGCCCAAGTATGGCTGCATCGAGAACACAGGAACAGGAGATCGCTTTGGCCCAGGAGCCAACAGTGAACTGGAGGCGTCATTCCCTATTCAAGATGTCCTAGAAAACTACATTTATTACTTTCAGAGCGTTCATGAAAGCATCGAGCCGACCCACGATGTGTTTAGTTTTTATGTGAGTGACGGAAGTGGTCGCTCTGAAATCCACAGCATCAATATCACCATTGAGAGGAAGAACGATGAGCCTCCCAGGATGACCTTGCGGCCCCTGGGCGTACGGCTGAGCTCGGGAGTGGCGATAAGCAATTCTTCCTTGAGTCTACAAGATCTGGACACCCCAGATAACGAGCTGATCTTTGTATTGATGAAAAAGCCTGACCATGGTCATCTACTCCGGAGGTCTACTGCCTCTGACCCTCTAGAAAATGGGACAGTCTTAGACCAAGGCTCCTCCTTCACCTATCAGGATGTCCTAGCTGGGCTGGTTGGCTACTTACCTGGTGATATCTATATGGCAGTCGATGAGTTCCGGTTCTCTCTCACGGATGGCCTCCATGTAGACACAGGAAGAATGGAGATCTATATAGAACTGCCATCAACCAACATTCCCCACTTGGCAATAAACCGAGGACTTCAGCTTTCAGCAGGGTCCGTAGCACGCATCACAGAGCAGCACTTGAAAGCAACAGACACTGACTCTGAGGCCGGCCAGGTTGTATACATCATGAAGGAAGACCCTGGTGCAGGGCGTCTGCTGATGGCTAAGGCTGACAACCTAGAGCAAATCTCTGTTAGAGGCCCCATCCGGAGCTTCACCCAGGCAGACGTTAGCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGATGGCTAAGGCTGACA
60.11
AGGCGCCTGAGGTAAGATTG
59.82
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGATGGCTAAGGCTGACA
60.11
CAGGCGCCTGAGGTAAGATT
59.82
159
PCR template sequence:
CCTGGTGCAGGGCGTCTGCTGATGGCTAAGGCTGACAACCTAGAGCAAATCTCTGTTAGAGGCCCCATCCGGAGCTTCACCCAGGCAGACGTTAGCCAAGGTGTCTAGTGCCACCAGTGCCCAGGAACACCTGGAAAGTCACATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-127-5p(2), mmu-miR-335-3p(2), mmu-miR-540-3p(2), mmu-miR-134-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-138-5p(1), mmu-miR-185-5p(1), mmu-miR-136-5p(1), mmu-miR-434-5p(1), mmu-miR-433-5p(1), mmu-miR-676-3p(1), mmu-miR-370-3p(1), mmu-miR-697(1), mmu-miR-490-3p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACGTTAGCCAAGGTGTCTAGTGCCA
siRNA sets
TGGCACTAGACACCTTGGC, TAGACACCTTGGCTAACGT, CTAGACACCTTGGCTAACG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRAS1_2155_ORF_1
1.149
0.288168401
0.999998286
2121
ORF sequence:
ATGTTCAACATTGCAATCTTACCTCAGGCGCCTGAGGCACCTAAACTCTCCTTGGGGACATCTCTCCATATGACCGCTCGTGAAGATGGCCTAAGTGTTATTCAGCCCCAGTCCTTATCCTTCGTGAAGGCAGAGAGTCCCAGTGGAAAGATTATATACAACATCACCGTACCTCTGCATCCAAATCAAGGTATCATTGAACACAGAGACCGGCCTCACTCTCCCATCCAGTATTTCACTCAGGAAGATATAAACCAAGGACAGATCATGTATCGCCCTCCTGTGGCACCTCCCCACCTCCAGGAGATCATGGCCTTCTCATTTGCTGGTCTCCCAGAATCAGTGAAATTTTACTTCACAGTTTCTGATGGGCAGCACACGAGTCCAGAGATGGCCCTCACCATTCACTTGCTGCACTCTGATCTGCAACCACCAGCATTTCAGGTCAAGGCTCCCCTGCTGGAAGTTAGCCCTGGAGGCCGGACCTCTTTAGGCCTGCAGTTGTTAGTGAGAGATGCTCAGGTGGTGCCAGAAGAGCTCTTCTTCCAGCTTCAGAAGTCTCCTCAGCATGGCATGCTCGTGAAGTACACGGCAAAGTCCTCGGTGACCATGGCTGCAGGTGACACTTTTACATATGATGAAGTCGAGAGAAACGTTCTGCAGTACGTACATGATGGGTCCTCTGCGTGGGAGGACAGCCTGGAGATCTCAGTCACAGATGGCCTGACAGTGACAACATCAGAAGTAAAAGTGGAGGTGTCCCCATCAGAGAACCGAGGGCCCAGGCTGGCCCCCGGCTCCTCCCTGAGCATGACGGTTGCTAGTCAACACACGGCCATAATCACCAGGTCACACCTTGCCTATGTGGATGATTCTTCCTCTGACCCAGAGATCTGGATTCGGTTAAGTTCTCTGCCACTGTATGGCGTACTCTTCAGAAGCTCAGGGCCTGACATGGACGAGCTCTCGGGGGATTCCAACTTCACCATGGAGGACATCAACAAGAAGAACATTAGGTACTCAGCTGTGTTTGAGACGGACGGGCATTCGGTTACAGATGGCTTCCACTTCTCTGTCTCAGACATGGACGGCAACCATGTGGACAACCAGGTCTTTACCATCACGGTCACTCCTGCCGAGAACCCTCCACACATCATTGCCTTTGCTGACCTTATCACGGTTGATGAGGGAGGGAGAGCACCACTCTCTCTCCACCACTTCTTTGCGACTGAAGATCAGGACAACCTTCAGGACGACGCTGTCATTAAACTAAGCGCTCTGCCCAAGTATGGCTGCATCGAGAACACAGGAACAGGAGATCGCTTTGGCCCAGGAGCCAACAGTGAACTGGAGGCGTCATTCCCTATTCAAGATGTCCTAGAAAACTACATTTATTACTTTCAGAGCGTTCATGAAAGCATCGAGCCGACCCACGATGTGTTTAGTTTTTATGTGAGTGACGGAAGTGGTCGCTCTGAAATCCACAGCATCAATATCACCATTGAGAGGAAGAACGATGAGCCTCCCAGGATGACCTTGCGGCCCCTGGGCGTACGGCTGAGCTCGGGAGTGGCGATAAGCAATTCTTCCTTGAGTCTACAAGATCTGGACACCCCAGATAACGAGCTGATCTTTGTATTGATGAAAAAGCCTGACCATGGTCATCTACTCCGGAGGTCTACTGCCTCTGACCCTCTAGAAAATGGGACAGTCTTAGACCAAGGCTCCTCCTTCACCTATCAGGATGTCCTAGCTGGGCTGGTTGGCTACTTACCTGGTGATATCTATATGGCAGTCGATGAGTTCCGGTTCTCTCTCACGGATGGCCTCCATGTAGACACAGGAAGAATGGAGATCTATATAGAACTGCCATCAACCAACATTCCCCACTTGGCAATAAACCGAGGACTTCAGCTTTCAGCAGGGTCCGTAGCACGCATCACAGAGCAGCACTTGAAAGCAACAGACACTGACTCTGAGGCCGGCCAGGTTGTATACATCATGAAGGAAGACCCTGGTGCAGGGCGTCTGCTGATGGCTAAGGCTGACAACCTAGAGCAAATCTCTGTTAGAGGCCCCATCCGGAGCTTCACCCAGGCAGACGTTAGCCAAGGTGTCTAG
Peptide sequence:
MFNIAILPQAPEAPKLSLGTSLHMTAREDGLSVIQPQSLSFVKAESPSGKIIYNITVPLHPNQGIIEHRDRPHSPIQYFTQEDINQGQIMYRPPVAPPHLQEIMAFSFAGLPESVKFYFTVSDGQHTSPEMALTIHLLHSDLQPPAFQVKAPLLEVSPGGRTSLGLQLLVRDAQVVPEELFFQLQKSPQHGMLVKYTAKSSVTMAAGDTFTYDEVERNVLQYVHDGSSAWEDSLEISVTDGLTVTTSEVKVEVSPSENRGPRLAPGSSLSMTVASQHTAIITRSHLAYVDDSSSDPEIWIRLSSLPLYGVLFRSSGPDMDELSGDSNFTMEDINKKNIRYSAVFETDGHSVTDGFHFSVSDMDGNHVDNQVFTITVTPAENPPHIIAFADLITVDEGGRAPLSLHHFFATEDQDNLQDDAVIKLSALPKYGCIENTGTGDRFGPGANSELEASFPIQDVLENYIYYFQSVHESIEPTHDVFSFYVSDGSGRSEIHSINITIERKNDEPPRMTLRPLGVRLSSGVAISNSSLSLQDLDTPDNELIFVLMKKPDHGHLLRRSTASDPLENGTVLDQGSSFTYQDVLAGLVGYLPGDIYMAVDEFRFSLTDGLHVDTGRMEIYIELPSTNIPHLAINRGLQLSAGSVARITEQHLKATDTDSEAGQVVYIMKEDPGAGRLLMAKADNLEQISVRGPIRSFTQADVSQGV
IRESfinder result
IRESfinder score
0.488710963
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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