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mmu_circFra10ac1_292
Basic information of circular RNA
PanCircBase ID:
mmu_circFra10ac1_292
mm39_Chr. Position ID:
chr19|38195710|38204355|-
Gene name:
Fra10ac1
Transcript:
ENSMUST00000067167.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,6098,7111,8568
Genomic length:
8645
Exon/intron sizes in nt:
46,85,84,77
Flanking exon/introns:
chr19:38193257-38195710|chr19:38204355-38207905
Splice length:
292
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TATCAAAGGCACACCAAGTTTGTAAACGACTATATTTTGTACTATGGTGGCAAAAGAGAGGACTTTAAGCGGTTGGGGGAGAATGATAAGACAGATCTGGATGTTATACGAGAAAATCATAGATTCCTTTGGAATGAGGAGGATGAAGCAGATATGACTTGGGAGAAGAGACTTGCAAAGAAATACTATGATAAATTATTCAAGGAATACTGCATAGCTGATCTCAGTAGATACAAAGAAAATAAGTTTGGATTTAGGTGGCGAATAGAAAAAGAAGTAATTTCAGGAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGCATAGCTGATCTCAGT
56.04
TCCCCCAACCGCTTAAAGTC
59.96
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGCATAGCTGATCTCAGT
56.04
CCCCAACCGCTTAAAGTCCT
59.96
162
PCR template sequence:
AAATTATTCAAGGAATACTGCATAGCTGATCTCAGTAGATACAAAGAAAATAAGTTTGGATTTAGGTGGCGAATAGAAAAAGAAGTAATTTCAGGAAAAGTATCAAAGGCACACCAAGTTTGTAAACGACTATATTTTGTACTATGGTGGCAAAAGAGAGGACTTTAAGCGGTTGGGGGAGAATGATAAGACAGATCTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTCAGGAAAAGTATCAAAGGCACA
siRNA sets
GTGCCTTTGATACTTTTCC, TGCCTTTGATACTTTTCCT, TGTGCCTTTGATACTTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFRA10AC1_292_ORF_1
0.4988
-0.223238891
0.023018924
207
ORF sequence:
ATGACTTGGGAGAAGAGACTTGCAAAGAAATACTATGATAAATTATTCAAGGAATACTGCATAGCTGATCTCAGTAGATACAAAGAAAATAAGTTTGGATTTAGGTGGCGAATAGAAAAAGAAGTAATTTCAGGAAAAGTATCAAAGGCACACCAAGTTTGTAAACGACTATATTTTGTACTATGGTGGCAAAAGAGAGGACTTTAA
Peptide sequence:
MTWEKRLAKKYYDKLFKEYCIADLSRYKENKFGFRWRIEKEVISGKVSKAHQVCKRLYFVLWWQKRGL
IRESfinder result
IRESfinder score
0.473193937
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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