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mmu_circFmnl2_984
Basic information of circular RNA
PanCircBase ID:
mmu_circFmnl2_984
mm39_Chr. Position ID:
chr2|53004866|53013585|+
Gene name:
Fmnl2
Transcript:
ENSMUST00000155586.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
17,18,19,20,21,22,23
Exon/intron offsets:
0,1985,3332,3509,5105,7633,8617
Genomic length:
8719
Exon/intron sizes in nt:
20,32,35,69,81,13,01,60,000
Flanking exon/introns:
chr2:53004248-53004866|chr2:53013585-53016024
Splice length:
984
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GACTTAAATGTGGACGAATTTGAAGAAATATTCAAGACAAAAGCCCAAGGTCCTGCCATTGATCTTTCTTCAAGCAAGCAGAAAATAACACAGAAGGCATCAAGTAAAGTGACATTATTAGAAGCAAATCGAGCCAAAAATCTTGCCATAACTTTAAGGAAAGCTGGGAAGTCTGCGGATGAGATATGTAAAGCTATTCATGTGTTTGACTTGAAGACGCTACCGGTTGACTTTGTTGAGTGTTTGATGAGGTTCTTGCCAACTGAGAATGAGGTCAAAGTGCTTCGGCTGTATGAGCGAGAGAGGAAGCCCTTGGAGAACTTGTCAGACGAGGACCGTTTCATGATGCAGTTCAGTAAGATTGAGCGGCTGCTGCAGAAGATGACCATCATGGCCTTCATTGGGAACTTCACAGAGAGCATTCAGATGCTGACTCCTCAACTTCACGCAATTATAGCAGCATCCGTCTCTATCAAGTCATCTCAAAAGCTCAAGAAGATTCTGGAGATCATCTTGGCCCTTGGAAACTATATGAATAGCAGCAAACGAGGAGCAGTCTATGGATTTAAACTTCAGAGTTTAGATCTGCTCTTAGATACAAAGTCGACAGACCGAAAGCAAACACTGTTGCACTATATATCAAATGTGGTAAAAGAAAAATATCAACAAGTTACTCTGTTTTATAATGAGCTTCATTACGTGGAAAAAGCTGCTGCAGTCTCCCTGGAGAACGTTCTGCTGGATGTGAAGGAGCTGCAGAGGGGGATGGATTTAACCAAGAGAGAGTATACCATGCATGACCACAACACCCTGCTGAAAGAGTTCCTCCTTCACAACGAGGGCAAGCTGAAGAAACTGCAGGAGGACGCGAAGATTGCTCAGGATGCCTTTGATGATGTGGTGAAATATTTTGGCGAAAATCCCAAGACAACTCCACCCTCCGTGTTCTTCCCTGTCTTTGTTCGATTTGTGAAAGCCTACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCTTTGATGATGTGGTGA
57.42
ATCAATGGCAGGACCTTGGG
60.03
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCTTTGATGATGTGGTGAA
58.13
ATCAATGGCAGGACCTTGGG
60.03
163
PCR template sequence:
TGCCTTTGATGATGTGGTGAAATATTTTGGCGAAAATCCCAAGACAACTCCACCCTCCGTGTTCTTCCCTGTCTTTGTTCGATTTGTGAAAGCCTACAAGGACTTAAATGTGGACGAATTTGAAGAAATATTCAAGACAAAAGCCCAAGGTCCTGCCATTGATCTTTCTTCAAGCAAGCAGAAAATAACACAGAAGGCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-205-5p(1), mmu-miR-217-5p(1), mmu-miR-127-5p(1), mmu-miR-483-5p(1), mmu-miR-301b-3p(1), mmu-miR-301a-3p(1), mmu-miR-532-5p(1), mmu-miR-3065-5p(1), mmu-miR-1904(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAAGCCTACAAGGACTTAAATGTGG
siRNA sets
ACATTTAAGTCCTTGTAGG, CATTTAAGTCCTTGTAGGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFMNL2_984_ORF_1
1.1176
0.313773418
0.999972427
1722
ORF sequence:
ATGAGGTTCTTGCCAACTGAGAATGAGGTCAAAGTGCTTCGGCTGTATGAGCGAGAGAGGAAGCCCTTGGAGAACTTGTCAGACGAGGACCGTTTCATGATGCAGTTCAGTAAGATTGAGCGGCTGCTGCAGAAGATGACCATCATGGCCTTCATTGGGAACTTCACAGAGAGCATTCAGATGCTGACTCCTCAACTTCACGCAATTATAGCAGCATCCGTCTCTATCAAGTCATCTCAAAAGCTCAAGAAGATTCTGGAGATCATCTTGGCCCTTGGAAACTATATGAATAGCAGCAAACGAGGAGCAGTCTATGGATTTAAACTTCAGAGTTTAGATCTGCTCTTAGATACAAAGTCGACAGACCGAAAGCAAACACTGTTGCACTATATATCAAATGTGGTAAAAGAAAAATATCAACAAGTTACTCTGTTTTATAATGAGCTTCATTACGTGGAAAAAGCTGCTGCAGTCTCCCTGGAGAACGTTCTGCTGGATGTGAAGGAGCTGCAGAGGGGGATGGATTTAACCAAGAGAGAGTATACCATGCATGACCACAACACCCTGCTGAAAGAGTTCCTCCTTCACAACGAGGGCAAGCTGAAGAAACTGCAGGAGGACGCGAAGATTGCTCAGGATGCCTTTGATGATGTGGTGAAATATTTTGGCGAAAATCCCAAGACAACTCCACCCTCCGTGTTCTTCCCTGTCTTTGTTCGATTTGTGAAAGCCTACAAGGACTTAAATGTGGACGAATTTGAAGAAATATTCAAGACAAAAGCCCAAGGTCCTGCCATTGATCTTTCTTCAAGCAAGCAGAAAATAACACAGAAGGCATCAAGTAAAGTGACATTATTAGAAGCAAATCGAGCCAAAAATCTTGCCATAACTTTAAGGAAAGCTGGGAAGTCTGCGGATGAGATATGTAAAGCTATTCATGTGTTTGACTTGAAGACGCTACCGGTTGACTTTGTTGAGTGTTTGATGAGGTTCTTGCCAACTGAGAATGAGGTCAAAGTGCTTCGGCTGTATGAGCGAGAGAGGAAGCCCTTGGAGAACTTGTCAGACGAGGACCGTTTCATGATGCAGTTCAGTAAGATTGAGCGGCTGCTGCAGAAGATGACCATCATGGCCTTCATTGGGAACTTCACAGAGAGCATTCAGATGCTGACTCCTCAACTTCACGCAATTATAGCAGCATCCGTCTCTATCAAGTCATCTCAAAAGCTCAAGAAGATTCTGGAGATCATCTTGGCCCTTGGAAACTATATGAATAGCAGCAAACGAGGAGCAGTCTATGGATTTAAACTTCAGAGTTTAGATCTGCTCTTAGATACAAAGTCGACAGACCGAAAGCAAACACTGTTGCACTATATATCAAATGTGGTAAAAGAAAAATATCAACAAGTTACTCTGTTTTATAATGAGCTTCATTACGTGGAAAAAGCTGCTGCAGTCTCCCTGGAGAACGTTCTGCTGGATGTGAAGGAGCTGCAGAGGGGGATGGATTTAACCAAGAGAGAGTATACCATGCATGACCACAACACCCTGCTGAAAGAGTTCCTCCTTCACAACGAGGGCAAGCTGAAGAAACTGCAGGAGGACGCGAAGATTGCTCAGGATGCCTTTGATGATGTGGTGAAATATTTTGGCGAAAATCCCAAGACAACTCCACCCTCCGTGTTCTTCCCTGTCTTTGTTCGATTTGTGAAAGCCTACAAG
Peptide sequence:
MRFLPTENEVKVLRLYERERKPLENLSDEDRFMMQFSKIERLLQKMTIMAFIGNFTESIQMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYQQVTLFYNELHYVEKAAAVSLENVLLDVKELQRGMDLTKREYTMHDHNTLLKEFLLHNEGKLKKLQEDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKAYKDLNVDEFEEIFKTKAQGPAIDLSSSKQKITQKASSKVTLLEANRAKNLAITLRKAGKSADEICKAIHVFDLKTLPVDFVECLMRFLPTENEVKVLRLYERERKPLENLSDEDRFMMQFSKIERLLQKMTIMAFIGNFTESIQMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYQQVTLFYNELHYVEKAAAVSLENVLLDVKELQRGMDLTKREYTMHDHNTLLKEFLLHNEGKLKKLQEDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKAYK
IRESfinder result
IRESfinder score
0.591981032
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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