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mmu_circFmn2_845_1
Basic information of circular RNA
PanCircBase ID:
mmu_circFmn2_845_1
mm39_Chr. Position ID:
chr1|174474671|174571872|+
Gene name:
Fmn2
Transcript:
ENSMUST00000030039.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,19537,47081,47326,48480,51663,52450,72739,97149
Genomic length:
97201
Exon/intron sizes in nt:
88,62,92,130,147,60,121,93,52
Flanking exon/introns:
chr1:174440217-174474671|chr1:174571872-174618823
Splice length:
845
Other database IDs:
circAtlas: mmu-Fmn2_0014
circBase: hsa_circ_0007019, circAtlas: hsa-FMN2_0004, CIRCpedia: HSA_CIRCpedia_30877
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTCAAGTTGCTTAGCAACAAAAGGTCACAAGCAGTAGGAATTCTAATGTCTAGTCTGCATTTAGATATGAAAGACATACAACATGCTGTTGTGAACTTGGACAACTCTGTGGTGGACCTGGAGACCCTTCAAGCTCTCTATGAGAATAGAGCACAGTCAGATGAACTGGAAAAAATTGAAAAGCACAGTCGATCTTCCAAAGACAAGGAAAACGCCAAGTCTCTCGACAAACCTGAACAGTTCCTGTATGAGCTGTCGCTAATCCCCAACTTCTCCGAGCGGGTCTTCTGTATCCTGTTTCAGTCCACATTTTCAGAGAGCATTTGCTCAATTCGTCGCAAGTTAGAATTGCTACAAAAACTGTGTGAGACCTTAAAAAATGGACCAGGGGTCATGCAAGTCCTGGGTTTAGTTCTAGCCTTTGGGAACTACATGAATGCTGGGAACAAGACCCGAGGACAGGCAGATGGCTTTGGACTAGACATTTTGCCCAAGCTGAAGGATGTCAAAAGCAGCGACAACAGCAGAAGCCTTTTGTCATATATTGTTTCATATTATCTTCGAAATTTTGATGAGGATGCTGGCAAAGAGCAGTGTGTCTTCCCGCTGGCAGAACCCCAGGAGCTTTTCCAGGCCTCACAGATGAAGTTTGAAGACTTCCAGAAAGACCTCAGAAAACTAAAGAAAGACCTGAAAGCCTGTGAGGCGGAAGCCGGTAAAGTGTACCAGGTGTCCTCTGCGGAGCATATGCAGCCTTTCAAGGAAAACATGGAGCAGTTTATCAGTCAAGCTAAAATTGACCAAGAGTCACAGGAGGCTGCCCTGACAGAGACTCATAAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCATATGCAGCCTTTCAAGGA
58.97
TCCTACTGCTTGTGACCTTTTGT
60.12
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCATATGCAGCCTTTCAAGG
58.97
TCCTACTGCTTGTGACCTTTTGT
60.12
142
PCR template sequence:
AGCATATGCAGCCTTTCAAGGAAAACATGGAGCAGTTTATCAGTCAAGCTAAAATTGACCAAGAGTCACAGGAGGCTGCCCTGACAGAGACTCATAAATGGTTGTCAAGTTGCTTAGCAACAAAAGGTCACAAGCAGTAGGAATTCTAATGTCTAGTCTGCATTTAGATATGAAAGACATACAACATGCTGTTGTGAACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-31-5p(1), mmu-miR-298-5p(1), mmu-miR-7a-5p(1), mmu-miR-758-3p(1), mmu-miR-297a-5p(1), mmu-miR-690(1), mmu-miR-342-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGACTCATAAATGGTTGTCAAGTTGC
siRNA sets
ACTTGACAACCATTTATGA, TGACAACCATTTATGAGTC, CTTGACAACCATTTATGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFMN2_845_1_ORF_1
1.1548
0.260244156
0.982603789
846
ORF sequence:
ATGGTTGTCAAGTTGCTTAGCAACAAAAGGTCACAAGCAGTAGGAATTCTAATGTCTAGTCTGCATTTAGATATGAAAGACATACAACATGCTGTTGTGAACTTGGACAACTCTGTGGTGGACCTGGAGACCCTTCAAGCTCTCTATGAGAATAGAGCACAGTCAGATGAACTGGAAAAAATTGAAAAGCACAGTCGATCTTCCAAAGACAAGGAAAACGCCAAGTCTCTCGACAAACCTGAACAGTTCCTGTATGAGCTGTCGCTAATCCCCAACTTCTCCGAGCGGGTCTTCTGTATCCTGTTTCAGTCCACATTTTCAGAGAGCATTTGCTCAATTCGTCGCAAGTTAGAATTGCTACAAAAACTGTGTGAGACCTTAAAAAATGGACCAGGGGTCATGCAAGTCCTGGGTTTAGTTCTAGCCTTTGGGAACTACATGAATGCTGGGAACAAGACCCGAGGACAGGCAGATGGCTTTGGACTAGACATTTTGCCCAAGCTGAAGGATGTCAAAAGCAGCGACAACAGCAGAAGCCTTTTGTCATATATTGTTTCATATTATCTTCGAAATTTTGATGAGGATGCTGGCAAAGAGCAGTGTGTCTTCCCGCTGGCAGAACCCCAGGAGCTTTTCCAGGCCTCACAGATGAAGTTTGAAGACTTCCAGAAAGACCTCAGAAAACTAAAGAAAGACCTGAAAGCCTGTGAGGCGGAAGCCGGTAAAGTGTACCAGGTGTCCTCTGCGGAGCATATGCAGCCTTTCAAGGAAAACATGGAGCAGTTTATCAGTCAAGCTAAAATTGACCAAGAGTCACAGGAGGCTGCCCTGACAGAGACTCATAAA
Peptide sequence:
MVVKLLSNKRSQAVGILMSSLHLDMKDIQHAVVNLDNSVVDLETLQALYENRAQSDELEKIEKHSRSSKDKENAKSLDKPEQFLYELSLIPNFSERVFCILFQSTFSESICSIRRKLELLQKLCETLKNGPGVMQVLGLVLAFGNYMNAGNKTRGQADGFGLDILPKLKDVKSSDNSRSLLSYIVSYYLRNFDEDAGKEQCVFPLAEPQELFQASQMKFEDFQKDLRKLKKDLKACEAEAGKVYQVSSAEHMQPFKENMEQFISQAKIDQESQEAALTETHK
IRESfinder result
IRESfinder score
0.54167008
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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