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mmu_circFkbp15_1759
Basic information of circular RNA
PanCircBase ID:
mmu_circFkbp15_1759
mm39_Chr. Position ID:
chr4|62232490|62258933|-
Gene name:
Fkbp15
Transcript:
ENSMUST00000084527.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5
Exon/intron offsets:
0,6705,7929,8943,9858,11805,13559,15094,17937,19429,19867,22147,22803,24232,25980,26368
Genomic length:
26443
Exon/intron sizes in nt:
172,103,95,108,110,115,107,103,108,58,140,147,69,150,99,75
Flanking exon/introns:
chr4:62230631-62232490|chr4:62258933-62263744
Splice length:
1759
Other database IDs:
circAtlas: hsa-FKBP15_0041, CIRCpedia: HSA_CIRCpedia_62373
Human Ortholog IDs:
Mature spliced Sequence:
TATAGGATTCTTCTTTACATCAGTCAGCAGCAGCCAGTGACTGTTGCTACAATTCATCTGAACTTTGAGCTAATGGTTCGGCCCAATAACTACAGCACCTTTTATGATGACCAGAGACAAAACTGGTCTATCATGTTTGAGTCAGAGAAGGCCGCTGTGTCATTCAATAAACAGGTGTGTGTGGCCAAGTGCAATAGCATCTCTTCCTTGGATGCAGTGCTGTGTCAGGACCTGGTTGCAGCAGAGGGCCCTGCTGTGGAAACTGGAGATTCTTTAGAAGTAGCCTATACGGGCTGGCTCCTTCAGAATCATGTGCTGGGCCAGGTTTTTGATTCTACTGCTAACAAAGATAAGCCGCTTCGCCTAAAATTAGGATCAGGAAAAGTTGTCAAGGGCTTAGAGGACGGGCTACTGGGCATGAAGAAAGGAGGAAAGCGCTTAATTATCACTCCTTCAGCCTGCGCTGCTGGCTCTGAAGGAGTAATAGGCTGGACTCAGCCAACAGACTCCATCCTGGTGTTTGAGGTAGAAGTTAGGCGGGTGAAGTTTGCCAGAGATTCTGGCTCTGATGGCCACAGTGTGAGCTCCCGGGATTCTGCAGCCCCTTCCCCCATCCCTGCTTCTGACAGCCTCTCTGCTGATCCTGTTGTGACACCCCTGCCATTGCCTCTCAAACCTGGGGAACCGGGTCTTCGTTCCAAATCTAACTCTCTCAGCGAACAGCTAACTGTAAATTCAAATCCTGATACGGTCAAGGCCAAGCTGATCTCGAGGATGGCTAAAATGGGCCAGCCCATGCTACCCATCCTTCCGCCACAGCTGGACTCCAATGACTCAGAAACCGAAGATGCAACTGTTCTGCGAGGAGCTGGGCAGTCCCTTGTGACACCATCTATCCAGCCTTCTCTTCAGCCAGCTCATCCGGTATTACCACAGATGGCCTCACAGGCACCTCAGCCATCTGGTTCTGGGCTCCAGACACCCTCTGCTGCCTTGATGCAAGCTGTGTCCCTTGATTCTCACTCGGCTGTATCTGGAAATGCACAGAACTTCCAGCCCTATGCAGGTGTGCAAGCCTATGCATATCCCCAGACACCCTCAGTTACTTCCCAGCTGCAGCCTGTTCGGCCCTTGTACCCAGCACCACTGTCCCAGGCTCCCCACTTTCAAGGATCAGGAGACATGATGTCCTTTCTCATGACTGAAGCCCGGCAACACAACACTGAAATTCGAATGGCAGTCAACAAAGTGGCTGATAAAATGGATCACCTCATGACTAAGGTGGAGGAGTTACAGAAGCATAGCTCTGGCAACTCCATGCTTCTTCCTAGCATGTCGGTCACAATGGAAACAAGCATGATTATGAGCAACATCCAGCGCATCATCCAGGAAAATGAGAGACTGAAGCAGGAGCTCCTTGAGAAAAGCAGTCGGATAGAAGAGCAGAATGACAAGATTAGTGACCTCATTGAACGCAACCAGAGATATGTTGAGCAGAGTAACCTGATGATGGAGAAGAGGAACAACTCGCTTCAAACAGCCACAGAAAACACACAGGCAAGAATATTGCATGCTGAACAAGAGAAGGCCAAGGTAACAGAAGAGTTAGCAGCAGCTACTGCACAGGTGTCTCACCTGCAGCTGAAAATGACGGCTCACCAGAAGAAGGAGACAGAATTGCAGTTGCAGCTGACAGACAACTTGAAGGAGACAGATCTTCTCAGGGGCCACGTCACCAGGCTCCAGGCTGACCTCTCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGATCTTCTCAGGGGCCAC
59.82
TGCTGTAGTTATTGGGCCGA
59.09
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGATCTTCTCAGGGGCCA
60.25
TGCTGTAGTTATTGGGCCGA
59.09
146
PCR template sequence:
CAGAAGAAGGAGACAGAATTGCAGTTGCAGCTGACAGACAACTTGAAGGAGACAGATCTTCTCAGGGGCCACGTCACCAGGCTCCAGGCTGACCTCTCGGTATAGGATTCTTCTTTACATCAGTCAGCAGCAGCCAGTGACTGTTGCTACAATTCATCTGAACTTTGAGCTAATGGTTCGGCCCAATAACTACAGCACCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-92a-3p(1), mmu-miR-18a-5p(1), mmu-miR-298-5p(1), mmu-miR-217-5p(1), mmu-miR-709(1), mmu-miR-25-3p(1), mmu-miR-351-5p(1), mmu-miR-363-3p(1), mmu-miR-382-5p(1), mmu-miR-362-5p(1), mmu-miR-490-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGACCTCTCGGTATAGGATTCTTC
siRNA sets
AAGAATCCTATACCGAGAG, AGAATCCTATACCGAGAGG, GAAGAATCCTATACCGAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFKBP15_1759_ORF_1
1.0534
0.256826355
0.999949511
1692
ORF sequence:
ATGGTTCGGCCCAATAACTACAGCACCTTTTATGATGACCAGAGACAAAACTGGTCTATCATGTTTGAGTCAGAGAAGGCCGCTGTGTCATTCAATAAACAGGTGTGTGTGGCCAAGTGCAATAGCATCTCTTCCTTGGATGCAGTGCTGTGTCAGGACCTGGTTGCAGCAGAGGGCCCTGCTGTGGAAACTGGAGATTCTTTAGAAGTAGCCTATACGGGCTGGCTCCTTCAGAATCATGTGCTGGGCCAGGTTTTTGATTCTACTGCTAACAAAGATAAGCCGCTTCGCCTAAAATTAGGATCAGGAAAAGTTGTCAAGGGCTTAGAGGACGGGCTACTGGGCATGAAGAAAGGAGGAAAGCGCTTAATTATCACTCCTTCAGCCTGCGCTGCTGGCTCTGAAGGAGTAATAGGCTGGACTCAGCCAACAGACTCCATCCTGGTGTTTGAGGTAGAAGTTAGGCGGGTGAAGTTTGCCAGAGATTCTGGCTCTGATGGCCACAGTGTGAGCTCCCGGGATTCTGCAGCCCCTTCCCCCATCCCTGCTTCTGACAGCCTCTCTGCTGATCCTGTTGTGACACCCCTGCCATTGCCTCTCAAACCTGGGGAACCGGGTCTTCGTTCCAAATCTAACTCTCTCAGCGAACAGCTAACTGTAAATTCAAATCCTGATACGGTCAAGGCCAAGCTGATCTCGAGGATGGCTAAAATGGGCCAGCCCATGCTACCCATCCTTCCGCCACAGCTGGACTCCAATGACTCAGAAACCGAAGATGCAACTGTTCTGCGAGGAGCTGGGCAGTCCCTTGTGACACCATCTATCCAGCCTTCTCTTCAGCCAGCTCATCCGGTATTACCACAGATGGCCTCACAGGCACCTCAGCCATCTGGTTCTGGGCTCCAGACACCCTCTGCTGCCTTGATGCAAGCTGTGTCCCTTGATTCTCACTCGGCTGTATCTGGAAATGCACAGAACTTCCAGCCCTATGCAGGTGTGCAAGCCTATGCATATCCCCAGACACCCTCAGTTACTTCCCAGCTGCAGCCTGTTCGGCCCTTGTACCCAGCACCACTGTCCCAGGCTCCCCACTTTCAAGGATCAGGAGACATGATGTCCTTTCTCATGACTGAAGCCCGGCAACACAACACTGAAATTCGAATGGCAGTCAACAAAGTGGCTGATAAAATGGATCACCTCATGACTAAGGTGGAGGAGTTACAGAAGCATAGCTCTGGCAACTCCATGCTTCTTCCTAGCATGTCGGTCACAATGGAAACAAGCATGATTATGAGCAACATCCAGCGCATCATCCAGGAAAATGAGAGACTGAAGCAGGAGCTCCTTGAGAAAAGCAGTCGGATAGAAGAGCAGAATGACAAGATTAGTGACCTCATTGAACGCAACCAGAGATATGTTGAGCAGAGTAACCTGATGATGGAGAAGAGGAACAACTCGCTTCAAACAGCCACAGAAAACACACAGGCAAGAATATTGCATGCTGAACAAGAGAAGGCCAAGGTAACAGAAGAGTTAGCAGCAGCTACTGCACAGGTGTCTCACCTGCAGCTGAAAATGACGGCTCACCAGAAGAAGGAGACAGAATTGCAGTTGCAGCTGACAGACAACTTGAAGGAGACAGATCTTCTCAGGGGCCACGTCACCAGGCTCCAGGCTGACCTCTCGGTATAG
Peptide sequence:
MVRPNNYSTFYDDQRQNWSIMFESEKAAVSFNKQVCVAKCNSISSLDAVLCQDLVAAEGPAVETGDSLEVAYTGWLLQNHVLGQVFDSTANKDKPLRLKLGSGKVVKGLEDGLLGMKKGGKRLIITPSACAAGSEGVIGWTQPTDSILVFEVEVRRVKFARDSGSDGHSVSSRDSAAPSPIPASDSLSADPVVTPLPLPLKPGEPGLRSKSNSLSEQLTVNSNPDTVKAKLISRMAKMGQPMLPILPPQLDSNDSETEDATVLRGAGQSLVTPSIQPSLQPAHPVLPQMASQAPQPSGSGLQTPSAALMQAVSLDSHSAVSGNAQNFQPYAGVQAYAYPQTPSVTSQLQPVRPLYPAPLSQAPHFQGSGDMMSFLMTEARQHNTEIRMAVNKVADKMDHLMTKVEELQKHSSGNSMLLPSMSVTMETSMIMSNIQRIIQENERLKQELLEKSSRIEEQNDKISDLIERNQRYVEQSNLMMEKRNNSLQTATENTQARILHAEQEKAKVTEELAAATAQVSHLQLKMTAHQKKETELQLQLTDNLKETDLLRGHVTRLQADLSV
IRESfinder result
IRESfinder score
0.458740399
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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