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mmu_circFer1l6_737
Basic information of circular RNA
PanCircBase ID:
mmu_circFer1l6_737
mm39_Chr. Position ID:
chr15|58513670|58520643|+
Gene name:
Fer1l6
Transcript:
ENSMUST00000161028.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
35,36,37,38,39
Exon/intron offsets:
0,1579,2040,5094,6809
Genomic length:
6973
Exon/intron sizes in nt:
143,89,99,242,164
Flanking exon/introns:
chr15:58512098-58513670|chr15:58520643-58532601
Splice length:
737
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGGAGATGGTGGAGTCTTATGAACACTTGGCCCTCAAGGTGTTACACTCTTGGGAGGACATCCCTGAGGCTGGGTGGAGGCTGGTTCCTGAGCACATAGAAACAAGGCCACTCTACCACAAGGATAAACCAGGAATGGAGCAGGGCCGCCTACAGATGTGGGTGGATATGTTTCCCAAAGACACACCTCAGCTTGGACCTCCAGTTGACATTTCACCAAGGAAACCCAAAGGATACGAGCTGAGGGTGACCATCTGGAACACTGAAGATGTTATTTTAGAAGATGAAAATATCTTCACGGGTCAAAAATCAAGCGATATTTATGTCAAAGGGTGGATCAAGGGCCTGGAGGACGACAGGCAGGAGACAGATGTGCACTACAACTCCCTGACTGGAGAAGGCAACTTCAACTGGCGCTTCCTGTTTCCATTCCAGTACCTGCCCGCTGAAAAGCAGATGGTCATCAGCAAGCGGGAGAACATCTTCTCCCTGGAGAAGATGGAATGTAAGACCCCGGCCGTGCTGGTGCTGCAGGTCTGGGATTTTGAAAGACTGTCCTCAGATGACTTCCTGGGCTCCCTGGAAATGAACCTTAACAGCTTCCCTCGGGCAGCCAAGTCCGCGAAAGCCTGCGATCTGTCCAAGTTTGAAAATGCAGTCCAGGAGAACAAGATCTCCATATTCCAGCAAAAGCGGGTGCGTGGCTGGTGGCCTTTTGCAAAGAGCAAAGAGCTCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCATATTCCAGCAAAAGCGGG
59.93
TATGTGCTCAGGAACCAGCC
59.75
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATTCCAGCAAAAGCGGGTG
58.83
TATGTGCTCAGGAACCAGCC
59.75
157
PCR template sequence:
GTCCAAGTTTGAAAATGCAGTCCAGGAGAACAAGATCTCCATATTCCAGCAAAAGCGGGTGCGTGGCTGGTGGCCTTTTGCAAAGAGCAAAGAGCTCACGAGGAGATGGTGGAGTCTTATGAACACTTGGCCCTCAAGGTGTTACACTCTTGGGAGGACATCCCTGAGGCTGGGTGGAGGCTGGTTCCTGAGCACATAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-203-3p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAAGAGCTCACGAGGAGATGGTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFER1L6_737_ORF_1
1.0337
0.29425108
0.979052774
831
ORF sequence:
ATGGTGGAGTCTTATGAACACTTGGCCCTCAAGGTGTTACACTCTTGGGAGGACATCCCTGAGGCTGGGTGGAGGCTGGTTCCTGAGCACATAGAAACAAGGCCACTCTACCACAAGGATAAACCAGGAATGGAGCAGGGCCGCCTACAGATGTGGGTGGATATGTTTCCCAAAGACACACCTCAGCTTGGACCTCCAGTTGACATTTCACCAAGGAAACCCAAAGGATACGAGCTGAGGGTGACCATCTGGAACACTGAAGATGTTATTTTAGAAGATGAAAATATCTTCACGGGTCAAAAATCAAGCGATATTTATGTCAAAGGGTGGATCAAGGGCCTGGAGGACGACAGGCAGGAGACAGATGTGCACTACAACTCCCTGACTGGAGAAGGCAACTTCAACTGGCGCTTCCTGTTTCCATTCCAGTACCTGCCCGCTGAAAAGCAGATGGTCATCAGCAAGCGGGAGAACATCTTCTCCCTGGAGAAGATGGAATGTAAGACCCCGGCCGTGCTGGTGCTGCAGGTCTGGGATTTTGAAAGACTGTCCTCAGATGACTTCCTGGGCTCCCTGGAAATGAACCTTAACAGCTTCCCTCGGGCAGCCAAGTCCGCGAAAGCCTGCGATCTGTCCAAGTTTGAAAATGCAGTCCAGGAGAACAAGATCTCCATATTCCAGCAAAAGCGGGTGCGTGGCTGGTGGCCTTTTGCAAAGAGCAAAGAGCTCACGAGGAGATGGTGGAGTCTTATGAACACTTGGCCCTCAAGGTGTTACACTCTTGGGAGGACATCCCTGAGGCTGGGTGGAGGCTGGTTCCTGAGCACATAG
Peptide sequence:
MVESYEHLALKVLHSWEDIPEAGWRLVPEHIETRPLYHKDKPGMEQGRLQMWVDMFPKDTPQLGPPVDISPRKPKGYELRVTIWNTEDVILEDENIFTGQKSSDIYVKGWIKGLEDDRQETDVHYNSLTGEGNFNWRFLFPFQYLPAEKQMVISKRENIFSLEKMECKTPAVLVLQVWDFERLSSDDFLGSLEMNLNSFPRAAKSAKACDLSKFENAVQENKISIFQQKRVRGWWPFAKSKELTRRWWSLMNTWPSRCYTLGRTSLRLGGGWFLST
IRESfinder result
IRESfinder score
0.468843534
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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