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mmu_circFbxw7_1933
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxw7_1933
mm39_Chr. Position ID:
chr3|84810807|84883691|+
Gene name:
Fbxw7
Transcript:
ENSMUST00000107679.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
4,5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,51374,55003,61747,63939,65664,67295,68874,70902,72673
Genomic length:
72884
Exon/intron sizes in nt:
579,83,142,135,124,137,114,182,226,211
Flanking exon/introns:
chr3:84771507-84810807|chr3:84883691-84884579
Splice length:
1933
Other database IDs:
circAtlas: mmu-Fbxw7_0004
Human Ortholog IDs:
Mature spliced Sequence:
AATGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCTTTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTTCCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACATCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAAACCCTAGGCCTGGAGACCAGAACGATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAAATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAAGAGGAAGAGGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACATACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCATTCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCACCCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGATGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAGCGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAAATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATACACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTAGTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACCGGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGTACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGATATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATGGCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCTACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACCAGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTGTCTCTGGGAATGCAGA
58.74
CCCACAGAGAGCAGTTCCTG
60.04
172
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTTGTCTCTGGGAATGCAGA
59.36
CCCACAGAGAGCAGTTCCTG
60.04
173
PCR template sequence:
GGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGAATGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCTTTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-134-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-30c-5p(1), mmu-miR-143-3p(1), mmu-miR-339-5p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-301b-3p(1), mmu-miR-382-5p(1), mmu-miR-666-5p(1), mmu-miR-335-3p(1), mmu-miR-193b-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAACTTTGCAAGAATGTGAAAACCT
siRNA sets
TTTTCACATTCTTGCAAAG, AGGTTTTCACATTCTTGCA, TTTCACATTCTTGCAAAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBXW7_1933_ORF_1
0.8353
0.134515442
0.999985859
1995
ORF sequence:
ATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGAATGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCTTTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTTCCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACATCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAAACCCTAGGCCTGGAGACCAGAACGATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAAATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAAGAGGAAGAGGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACATACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCATTCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCACCCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGATGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAGCGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAAATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATACACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTAGTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACCGGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGTACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGATATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATGGCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCTACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACCAGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAA
Peptide sequence:
MQILQLRSGISKQDSVYKLCKNVKTFASSDSLAKVQEVASWLLEMNQELLSVGSKRRRTGGSLRGNASSSQVDEGQMNRVVEEDPQQQARHQEEEHTARNGELVGANPRPGDQNDTQQGQVEENNNRFISVDEDSSGNQEEQEEDEEHAGEQEEEEEEEEEEEEMDQESDDFDPSDDSSREDEHTHNSNVTNCSSVSDLPAHQLSSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSDYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKIIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQ
IRESfinder result
IRESfinder score
0.478582815
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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