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mmu_circFbxo3_766
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxo3_766
mm39_Chr. Position ID:
chr2|103876002|103885371|+
Gene name:
Fbxo3
Transcript:
ENSMUST00000028603.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
5,6,7,8,9,10
Exon/intron offsets:
0,2276,4613,5476,7707,9178
Genomic length:
9369
Exon/intron sizes in nt:
2,05,46,85,12,31,16,190
Flanking exon/introns:
chr2:103873363-103876002|chr2:103885371-103890226
Splice length:
766
Other database IDs:
circAtlas: mmu-Fbxo3_0009
Human Ortholog IDs:
Mature spliced Sequence:
GTTGCTGGGAAGTATGGCACTGTCCAATCACTACCGCTCTGAAGATCTACTAGATGTTGATACTGCTGCAGGAGGATTTCAGCAGAGACAGGGACTGAAATACTGTCTCCCTTTAACCTTTTGCATACACACCGGCTTGAGTCAGTACATAGCTGTGGAGGCTGCAGAGGGGCGGAATAAGAACGAAGTGTTCTACCAATGTCCAGACCAAATGGCTCGGAATCCTGCTGCTATTGACATGTTTATCATAGGTGCTACTTTTACCGACTGGTTTACATCCTATGTCAACAATGTTGTATCAGGTGGTTTCCCCATCATCAGAGACCAGATTTTCAGATATATTCATGATCCAGAATGTGTGGCAACAACTGGAGATATTACAGTTTCAGTGTCCACATCGTTTCTGCCAGAACTCAGCTCTGTGCATCCGCCACACTATTTCTTCACATACCGAATCAGGATTGAAATGTCAAGGGATGCTCTTCCTGAGAAGGCCTGTCAGTTGGACAGTCGCTACTGGAGGATAACAAACGCTAAAGGGGATGTGGAAGAAGTCCAGGGACCTGGAGTAGTCGGTGAATTTCCAATTATTAGCCCAGGTCGGATATATGAATACACAAGCTGTACCACATTTTCTACAACATCAGGCTACATGGAAGGGTATTACACCTTCCATTTTCTGTACTTTAAGGACAAGGTCTTTAATGTTGCCATCCCCCGATTCCACATGGCATGTCCAACATTCAGGGTGTCTATAGCTCGATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AATGTTGCCATCCCCCGATT
60.03
TCAGTCCCTGTCTCTGCTGA
59.89
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGTTGCCATCCCCCGATTC
60.47
TCAGTCCCTGTCTCTGCTGA
59.89
160
PCR template sequence:
CACCTTCCATTTTCTGTACTTTAAGGACAAGGTCTTTAATGTTGCCATCCCCCGATTCCACATGGCATGTCCAACATTCAGGGTGTCTATAGCTCGATTGGTTGCTGGGAAGTATGGCACTGTCCAATCACTACCGCTCTGAAGATCTACTAGATGTTGATACTGCTGCAGGAGGATTTCAGCAGAGACAGGGACTGAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(2), mmu-miR-339-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TATAGCTCGATTGGTTGCTGGGAAGT
siRNA sets
TTCCCAGCAACCAATCGAG, ACTTCCCAGCAACCAATCG, CTTCCCAGCAACCAATCGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBXO3_766_ORF_1
0.6183
0.093327761
0.879682406
795
ORF sequence:
ATGGCACTGTCCAATCACTACCGCTCTGAAGATCTACTAGATGTTGATACTGCTGCAGGAGGATTTCAGCAGAGACAGGGACTGAAATACTGTCTCCCTTTAACCTTTTGCATACACACCGGCTTGAGTCAGTACATAGCTGTGGAGGCTGCAGAGGGGCGGAATAAGAACGAAGTGTTCTACCAATGTCCAGACCAAATGGCTCGGAATCCTGCTGCTATTGACATGTTTATCATAGGTGCTACTTTTACCGACTGGTTTACATCCTATGTCAACAATGTTGTATCAGGTGGTTTCCCCATCATCAGAGACCAGATTTTCAGATATATTCATGATCCAGAATGTGTGGCAACAACTGGAGATATTACAGTTTCAGTGTCCACATCGTTTCTGCCAGAACTCAGCTCTGTGCATCCGCCACACTATTTCTTCACATACCGAATCAGGATTGAAATGTCAAGGGATGCTCTTCCTGAGAAGGCCTGTCAGTTGGACAGTCGCTACTGGAGGATAACAAACGCTAAAGGGGATGTGGAAGAAGTCCAGGGACCTGGAGTAGTCGGTGAATTTCCAATTATTAGCCCAGGTCGGATATATGAATACACAAGCTGTACCACATTTTCTACAACATCAGGCTACATGGAAGGGTATTACACCTTCCATTTTCTGTACTTTAAGGACAAGGTCTTTAATGTTGCCATCCCCCGATTCCACATGGCATGTCCAACATTCAGGGTGTCTATAGCTCGATTGGTTGCTGGGAAGTATGGCACTGTCCAATCACTACCGCTCTGA
Peptide sequence:
MALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAVEAAEGRNKNEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVNNVVSGGFPIIRDQIFRYIHDPECVATTGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSRDALPEKACQLDSRYWRITNAKGDVEEVQGPGVVGEFPIISPGRIYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKVFNVAIPRFHMACPTFRVSIARLVAGKYGTVQSLPL
IRESfinder result
IRESfinder score
0.56515591
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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