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mmu_circFbxo34_355
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxo34_355
mm39_Chr. Position ID:
chr14|47744721|47746686|+
Gene name:
Fbxo34
Transcript:
ENSMUST00000228019.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,1792
Genomic length:
1965
Exon/intron sizes in nt:
1,82,173
Flanking exon/introns:
chr14:47742589-47744721|chr14:47746686-47763661
Splice length:
355
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGACTGGTCAGAAAGGATCTTACTTCCAGGAATACTTGGTTGTCCTCCCAGGATACTATCCCCAGATGTCTCCCAACGACAGACTTGAGCAGTGCTGCCAAAGGCATGCCACTTCGATTTTCTAGCTGCCCTAAGTACTTGTGCCTGAGATCTGAGTGAGCCCAGTGGGAAGTAGCCTCCGGCTATGCATATGGAGAATGAAAATACTAGTGCACTGACTAGAAGTGTGGGTTGATGATCTGCTGAGGCACATGGATGCAGTGAGAACATTAATGAGCTGAAGGAGAGTAGGTCCCAAACTGCTTGAGTGCCATGGGACATCTTTAAAATGCAGAATATTCTTGAAAACATTATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCCCAAACTGCTTGAGTGC
59.69
GCTCAAGTCTGTCGTTGGGA
59.97
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCCCAAACTGCTTGAGTGC
59.69
GTCGTTGGGAGACATCTGGG
60.11
144
PCR template sequence:
ATGCAGTGAGAACATTAATGAGCTGAAGGAGAGTAGGTCCCAAACTGCTTGAGTGCCATGGGACATCTTTAAAATGCAGAATATTCTTGAAAACATTATGTGACTGGTCAGAAAGGATCTTACTTCCAGGAATACTTGGTTGTCCTCCCAGGATACTATCCCCAGATGTCTCCCAACGACAGACTTGAGCAGTGCTGCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-26a-5p(1), mmu-miR-148a-3p(1), mmu-miR-301b-3p(1), mmu-miR-107-3p(1), mmu-miR-301a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGAAAACATTATGTGACTGGTCAGAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.58983097
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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