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mmu_circFbxo21_353
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxo21_353
mm39_Chr. Position ID:
chr5|118115922|118124285|+
Gene name:
Fbxo21
Transcript:
ENSMUST00000202447.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,1883,8241
Genomic length:
8363
Exon/intron sizes in nt:
1,36,95,122
Flanking exon/introns:
chr5:118115104-118115922|chr5:118124285-118126842
Splice length:
353
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGCCCTCCCTGATGAAGCACTACAGCCCCACCGACTACGTCAATTGGTTAGAGGAATATAAAGTTCGGCAGAAAGCTGGGTTAGAAGCCCGGAAGATTGTAGCTTCCTTCTCCAAGCGGTTCTTTTCAGAGCACGTCCCCTGTAATGGCTTCAGTGACATTGAAAACCTTGAGGGCCCAGAGATCTTTTTTGAGGATGAGCTGGTGTGTATCCTAAACATGGAAGGAAGGAAAGCTTTGACCTGGAAATACTACGCAAAGAAAATTCTCTACTACCTGCGGCAGCAGAAAATCCTCAATAACCTCAAGGCGTTCCTGCAGCAGCCCGACGATTACGAGTCCTATCTCGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAACCTCAAGGCGTTCCTGC
60.32
TCTAACCCAGCTTTCTGCCG
60.04
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTACTACCTGCGGCAGC
59.93
CTAACCCAGCTTTCTGCCGA
60.04
171
PCR template sequence:
TACGCAAAGAAAATTCTCTACTACCTGCGGCAGCAGAAAATCCTCAATAACCTCAAGGCGTTCCTGCAGCAGCCCGACGATTACGAGTCCTATCTCGAAGGTGGCCCTCCCTGATGAAGCACTACAGCCCCACCGACTACGTCAATTGGTTAGAGGAATATAAAGTTCGGCAGAAAGCTGGGTTAGAAGCCCGGAAGATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1), mmu-miR-325-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TCCTATCTCGAAGGTGGCCCTCCCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBXO21_353_ORF_1
1.1418
0.368759094
0.71626257
354
ORF sequence:
ATGAAGCACTACAGCCCCACCGACTACGTCAATTGGTTAGAGGAATATAAAGTTCGGCAGAAAGCTGGGTTAGAAGCCCGGAAGATTGTAGCTTCCTTCTCCAAGCGGTTCTTTTCAGAGCACGTCCCCTGTAATGGCTTCAGTGACATTGAAAACCTTGAGGGCCCAGAGATCTTTTTTGAGGATGAGCTGGTGTGTATCCTAAACATGGAAGGAAGGAAAGCTTTGACCTGGAAATACTACGCAAAGAAAATTCTCTACTACCTGCGGCAGCAGAAAATCCTCAATAACCTCAAGGCGTTCCTGCAGCAGCCCGACGATTACGAGTCCTATCTCGAAGGTGGCCCTCCCTGA
Peptide sequence:
MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGGPP
IRESfinder result
IRESfinder score
0.585244752
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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