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mmu_circFbxl17_316
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxl17_316
mm39_Chr. Position ID:
chr17|63532019|63692047|-
Gene name:
Fbxl17
Transcript:
ENSMUST00000024761.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,131723,159920
Genomic length:
160028
Exon/intron sizes in nt:
7,71,31,108
Flanking exon/introns:
chr17:63387558-63532019|chr17:63692047-63778402
Splice length:
316
Other database IDs:
CIRCpedia: MMU_CIRCpedia_25517, circAtlas: mmu-Fbxl17_0011
CIRCpedia: HSA_CIRCpedia_49758
Human Ortholog IDs:
Mature spliced Sequence:
GTGACAGATCAGTCAGTGAAGGCATTTGCTGAGCACTGTCCTGAACTCCAGTATGTTGGCTTCATGGGTTGTTCAGTTACTTCCAAAGGAGTCATTCACTTAACCAAGCTAAGGAACCTGTCCAGCTTAGACCTCCGTCACATCACTGAACTGGATAATGAAACCGTGATGGAAATCGTCAAGAGGTGCAAAAATCTCAGCTCTCTGAATCTCTGTCTGAACTGGATCATAAACGACAGGTGTGTGGAGGTCATTGCAAAGGAAGGACAGAACCTGAAAGAGCTGTACCTGGTGTCCTGTAAGATCACTGATTATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAACGACAGGTGTGTGGAGG
60.18
ACTGAACAACCCATGAAGCCA
60.13
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTGTGTGGAGGTCATTGC
60.25
ACTGAACAACCCATGAAGCCA
60.13
156
PCR template sequence:
CTGAACTGGATCATAAACGACAGGTGTGTGGAGGTCATTGCAAAGGAAGGACAGAACCTGAAAGAGCTGTACCTGGTGTCCTGTAAGATCACTGATTATGGTGACAGATCAGTCAGTGAAGGCATTTGCTGAGCACTGTCCTGAACTCCAGTATGTTGGCTTCATGGGTTGTTCAGTTACTTCCAAAGGAGTCATTCACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-129-5p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCACTGATTATGGTGACAGATCAGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBXL17_316_ORF_1
1.1001
0.238517923
0.514469823
318
ORF sequence:
ATGGTGACAGATCAGTCAGTGAAGGCATTTGCTGAGCACTGTCCTGAACTCCAGTATGTTGGCTTCATGGGTTGTTCAGTTACTTCCAAAGGAGTCATTCACTTAACCAAGCTAAGGAACCTGTCCAGCTTAGACCTCCGTCACATCACTGAACTGGATAATGAAACCGTGATGGAAATCGTCAAGAGGTGCAAAAATCTCAGCTCTCTGAATCTCTGTCTGAACTGGATCATAAACGACAGGTGTGTGGAGGTCATTGCAAAGGAAGGACAGAACCTGAAAGAGCTGTACCTGGTGTCCTGTAAGATCACTGATTAT
Peptide sequence:
MVTDQSVKAFAEHCPELQYVGFMGCSVTSKGVIHLTKLRNLSSLDLRHITELDNETVMEIVKRCKNLSSLNLCLNWIINDRCVEVIAKEGQNLKELYLVSCKITDY
IRESfinder result
IRESfinder score
0.709927965
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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